Enhanced nonenzymatic glycation of eye lens proteins in experimental diabetes mellitus: An approach for the study of protein alterations as mediators of normal aging phenomena

Sensi, M.; Pricci, Flavia; Pugliese, Chiara; Rossi, Maria Grazia; Celi, Francesco S.; Cristina, A.; Mokano, S.; Andreani, D; Mario, U. Di

doi:10.1016/s0167-4943(05)80034-2

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Although most proteins in living systems turn over with sufficient rapidity to avoid significant accumulation of AGEs, some, such as lens crystallins, nerve myelin, and skin collagen, are long-lived, and AGEs accumulate in these proteins over a lifetime. 17,18 Because glycation is initiated by free-reducing sugars, diabetic subjects have an accelerated accumulation of AGEs in tissues, [19][20][21][22][23][24][25][26][27] which is postulated to be a key mechanism for the ocular, vascular, and other complications of diabetes. [28][29][30][31] Use of lens autofluorescence to screen for diabetes was studied by Koefoed Theil et al in 14 patients with newly diagnosed (<6 months) type 2 diabetes and 11 age-matched control subjects.…”

Section: Discussionmentioning

confidence: 99%

Measurement of Lens Autofluorescence Can Distinguish Subjects With Diabetes From Those Without

Cahn

Burd

Ignotz

et al. 2014

J Diabetes Sci Technol

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Lens autofluorescence is increased in patients with diabetes mellitus, but clinical application has been limited by the lack of an instrument suitable for routine clinical use. We investigate possible uses of a new scanning confocal biomicroscope (1) to identify subjects with undiagnosed type 2 diabetes and (2) as a marker for the progression of diabetes. One hundred seventyeight subjects self-reported as normal and 53 subjects physician-diagnosed with diabetes or prediabetes were recruited. Measurements were collected using a ClearPath DS-120 Lens Fluorescence Biomicroscope calibrated with standards traceable to National Institute of Standards and Technology (NIST). Fluorescence intensities were corrected for age by subtracting the value expected from a regression of intensity versus age for normal subjects. This "fluorescence deviation" showed progressively higher values for normal, prediabetes, type 2 diabetes, and type 1 diabetes and a high degree of predictability of diabetes diagnosis. A receiver operating characteristics curve was used to determine sensitivity and specificity for prediction of diabetes type 2. At a fluorescence deviation of 2500, a sensitivity of 67% at 94% specificity was observed detection of type 2 diabetes. The progressively higher fluorescence deviations are consistent with the physiological mechanisms of accumulation of fluorescent advanced glycation end products as the subject ages. The sensitivity and specificity performance of the lens autofluorescence test for type 2 diabetes is comparable to the performance of glucose threshold tests. The statistically significant difference between fluorescence deviations of normal and type 2 diabetes supports the feasibility of lens autofluorescence to screen subjects for undiagnosed type 2 diabetes. Ophthalmic practices are points of care at which there may be a public health benefit for screening patients for undiagnosed diabetes.

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Section: Discussionmentioning

confidence: 99%

Measurement of Lens Autofluorescence Can Distinguish Subjects With Diabetes From Those Without

Cahn

Burd

Ignotz

et al. 2014

J Diabetes Sci Technol

View full text Add to dashboard Cite

show abstract

“…[8][9][10][11][12][13] Because glycation is initiated by free reducing sugars, diabetes subjects have an accelerated accumulation of AGEs. [14][15][16][17][18][19][20][21][22] Thus lens autofluorescence, as a noninvasive measure of AGE accumulation, can be regarded as a measure of cumulative tissue damage due to elevated sugar in plasma and interstitial fluids.…”

Section: Introductionmentioning

confidence: 99%