Enhanced osteoblast functions and bactericidal effect of Ca and Ag dual-ion implanted surface layers on nanograined titanium alloys

Huang, Run; Han, Yong; Lu, Shemin

doi:10.1039/c4tb00124a

Cited by 26 publications

(13 citation statements)

References 69 publications

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“…The majority of the Ag nanoparticles are embedded in the interior of nano-TiO 2 beneath the surface, although some of them can still migrate into the surface. As a consequence, Ag ions cannot be released quickly from the Ag-embedded TiO 2 and the released amount is smaller than 10 ppb , which is less than the minimum bactericidal concentration of 100 ppb . Prolonged Ag + leaching renders the release-killing ability of the Ag-implanted surface while limited Ag + release contributes to a contact-killing as illustrated in Figure b.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection

Wang

Shi

et al. 2016

ACS Appl. Mater. Interfaces

100

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Periprosthetic joint infection (PJI) is one of the formidable and recalcitrant complications after orthopedic surgery, and inhibiting biofilm formation on the implant surface is considered crucial to prophylaxis of PJI. However, it has recently been demonstrated that free-floating biofilm-like aggregates in the local body fluid and bacterial colonization on the implant and peri-implant tissues can coexist and are involved in the pathogenesis of PJI. An effective surface with both contact-killing and release-killing antimicrobial capabilities can potentially abate these concerns and minimize PJI caused by adherent/planktonic bacteria. Herein, Ag nanoparticles (NPs) are embedded in titania (TiO2) nanotubes by anodic oxidation and plasma immersion ion implantation (PIII) to form a contact-killing surface. Vancomycin is then incorporated into the nanotubes by vacuum extraction and lyophilization to produce the release-killing effect. A novel clinical PJI model system involving both in vitro and in vivo use of methicillin-resistant Staphylococcus aureus (MRSA) ST239 is established to systematically evaluate the antibacterial properties of the hybrid surface against planktonic and sessile bacteria. The vancomycin-loaded and Ag-implanted TiO2 nanotubular surface exhibits excellent antimicrobial and antibiofilm effects against planktonic/adherent bacteria without appreciable silver ion release. The fibroblasts/bacteria cocultures reveal that the surface can help fibroblasts to combat bacteria. We first utilize the nanoarchitecture of implant surface as a bridge between the inorganic bactericide (Ag NPs) and organic antibacterial agent (vancomycin) to achieve total victory in the battle of PJI. The combination of contact-killing and release-killing together with cell-assisting function also provides a novel and effective strategy to mitigate bacterial infection and biofilm formation on biomaterials and has large potential in orthopedic applications.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection

Wang

Shi

et al. 2016

ACS Appl. Mater. Interfaces

100

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“…Ag-PIII has also been combined with other elemental implantation, including magnesium, zinc, and calcium, for additional antibacterial and osteogenic efficacy. [93, 96, 97] …”

Section: Challenges and Potential Solutions For Bacterial Infectiomentioning

confidence: 99%

Multifunctional coatings to simultaneously promote osseointegration and prevent infection of orthopaedic implants

et al. 2016

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The two leading causes of failure for joint arthroplasty prostheses are aseptic loosening and periprosthetic joint infection. With the number of primary and revision joint replacement surgeries on the rise, strategies to mitigate these failure modes have become increasingly important. Much of the recent work in this field has focused on the design of coatings either to prevent infection while ignoring bone mineralization or vice versa, to promote osseointegration while ignoring microbial susceptibility. However, both coating functions are required to achieve long-term success of the implant; therefore, these two modalities must be evaluated in parallel during the development of new orthopaedic coating strategies. In this review, we discuss recent progress and future directions for the design of multifunctional orthopaedic coatings that can inhibit microbial cells while still promoting osseointegration.

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“…The incidence of biomaterial-associated infection (BAI) in orthopaedics has been reported to be between 2% and 5% in recent years 5 . Accordingly, there is an urgent need to improve the surface performance of Ti-based implants to hinder the formation of pathogen biofilms and promote tissue bio-integration with tissue 6 7 .…”

mentioning

confidence: 99%

Silver-nanoparticles-modified biomaterial surface resistant to staphylococcus: new insight into the antimicrobial action of silver

Wang

Guo

et al. 2016

Sci Rep

100

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Titanium implants are widely used clinically, but postoperative implant infection remains a potential severe complication. The purpose of this study was to investigate the antibacterial activity of nano-silver(Ag)-functionalized Ti surfaces against epidemic Staphylococcus from the perspective of the regulation of biofilm-related genes and based on a bacteria-cell co-culture study. To achieve this goal, two representative epidemic Staphylococcus strains, Staphylococcus epidermidis (S. epidermidis, RP62A) and Staphylococcus aureus (S. aureus, USA 300), were used, and it was found that an Ag-nanoparticle-modified Ti surface could regulate the expression levels of biofilm-related genes (icaA and icaR for S. epidermidis; fnbA and fnbB for S. aureus) to inhibit bacterial adhesion and biofilm formation. Moreover, a novel bacteria-fibroblast co-culture study revealed that the incorporation of Ag nanoparticles on such a surface can help mammalian cells to survive, adhere and spread more successfully than Staphylococcus. Therefore, the modified surface was demonstrated to possess a good anti-infective capability against both sessile bacteria and planktonic bacteria through synergy between the effects of Ag nanoparticles and ion release. This work provides new insight into the antimicrobial action and mechanism of Ag-nanoparticle-functionalized Ti surfaces with bacteria-killing and cell-assisting capabilities and paves the way towards better satisfying the clinical needs.

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Enhanced osteoblast functions and bactericidal effect of Ca and Ag dual-ion implanted surface layers on nanograined titanium alloys

Cited by 26 publications

References 69 publications

Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection

Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection

Multifunctional coatings to simultaneously promote osseointegration and prevent infection of orthopaedic implants

Silver-nanoparticles-modified biomaterial surface resistant to staphylococcus: new insight into the antimicrobial action of silver

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