Enhanced peroxidase production by suspension culture of carrot compact callus aggregates

Xu, J. F.; Sun, Yan; Su, Zhiguo

doi:10.1016/s0168-1656(98)00114-x

Cited by 9 publications

(3 citation statements)

References 11 publications

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“…Cell divisions occurred frequently at the periphery, which is responsible for biomass increase (Xu et al 1998b). Georget et al (2000) found that type II aggregates in a banana suspension culture, which correspond to the 2-5 mm aggregates in our present study, were not only compact in appearance but also composed mainly of embryogenic cells.…”

Section: General Considerationssupporting

confidence: 64%

Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa

Zhao

Huang

et al. 2005

Biotechnol Lett

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Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with the sizes of compact callus aggregates (CCA) and cellular viability. In the red cell line, the sizes of CCA were predominantly of 2-4 mm diameter and accounted for 64% biomass. This line had a sustained cell viability over 10 successive sub-cultures.

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Section: General Considerationssupporting

confidence: 64%

Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa

Zhao

Huang

et al. 2005

Biotechnol Lett

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“…It has been demonstrated previously that peroxidase activity varied greatly in cell cultures of different plants: high peroxidase activities were demonstrated for both callus and suspension cultures of Raphanus sativus (Moreno et al 1989), while only trace peroxidase activity was detected in dispersed cell cultures of Daucus carota (Xu et al 1998). It was also shown that peroxidase activity was significantly higher in organogenic and embryogenic callus cultures compared with undifferentiated callus lines of Panax ginseng and Citrus sinensis (Bonfill et al 2003;Kochba et al 1977).…”

Section: Discussionmentioning

confidence: 90%

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

et al. 2012

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Here, seven new class III peroxidase genes of Rubia cordifolia L., RcPrx01-RcPrx07, were isolated and characterized. Expression of the Prx genes was studied in R. cordifolia aerial organs as well as in cells transformed with the rolB and rolC genes of Agrobacterium rhizogenes and cells transformed with the wild-type A. rhizogenes A4 strain. In rolC- and rolB-transformed cells, the rol genes were expressed under the control of the 35S promoter, whereas in A. rhizogenes A4-transformed cells the rol genes were expressed under the control of their native promoters. All studied peroxidase genes were greatly upregulated in rolB-overexpressing cells. In contrast, overexpression of the rolC gene and expression of the rol genes under the control of their native promoters had little effect on the abundance of peroxidase transcripts. In accordance with this observation, peroxidase activity was substantially increased in rolB cells and was slightly affected in other transformed cells. Our results indicate that rolB strictly affects the regulation of a set of seven R. cordifolia class III peroxidases.

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“…But, their activities were, at least, one-seventh of the basil crude extract (106.93 AU mg − 1 ) regardless of the employed electron-donors. Interestingly, the callus of carrot (D. carota) had already been examined as a candidate source of plant POX (Xu et al 1998). But the reported POX activity of its crude extract (54.0 AU/g of FW) is about one-third of the basil callus extract (Table 2).…”

Section: Puri Cation Of Obpoxmentioning

confidence: 99%

A potent peroxidase from solid cell culture of Ocimum basilicum with high sensitivity for blood glucose determination

Mohammadnejad

Asl

Rasoulian

et al. 2021

Plant Cell Tiss Organ Cult

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Objectives Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast period of 2020 -2025. Nonetheless, the large-scale POX preparation still relies on the extraction from agricultural products, while there is an accumulative driving force toward employing biotechnological processes with agricultural hassle free identity. In pursuit of this trend; Results, A novel heme peroxidase was puri ed to homogeneity (MW of 40 kD) from the callus culture of Ocimum basilicum L. in darkness on Murashige-Skoog medium supplemented by 2,4dichlorophenoxyacetic acid (10 -6 M) and kinetin (10 -5 M). The highest activity of the puri ed peroxidase (ObPOX) was observed in Tris-base buffer at pH 7.5 and 80 °C. ObPOX showed high stability over pH(s) 5 to 7.5 and temperatures of 15 to 60 °C. ObPOX speci c activity was 1245.142 AU mg -1 in the presence of phenol, 4 times higher than that of HRP. ObPOX showed moderate a nity for guaiacol (K m = 21.5 mM), but obtained an exceptionally high speci city constant (k cat /K m = 66743.7 s -1 M -1 ) for GASA (4-[(4-Hydroxy-3-methoxyphenyl) azo]-benzenesulfonic acid), the introduced substrate for determination of blood sugar. Applying ObPOX instead of HRP in glucose measurements of the real samples improved the regression constant of the correlation diagram between the tests and the lab results from 0.958 to 0.981.Conclusion Physicochemical properties of ObPOX as well as the growth rate of basil callus (5.04 g L -1 per day) and the yield of ObPOX production (35 mg per 100 g dry biomass per subculture) designates O. basilicum cell culture for large-scale production of a robust peroxidase.

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Enhanced peroxidase production by suspension culture of carrot compact callus aggregates

Cited by 9 publications

References 11 publications

Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa

Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

A potent peroxidase from solid cell culture of Ocimum basilicum with high sensitivity for blood glucose determination

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