Enhanced phenol degradation in coking wastewater by immobilized laccase on magnetic mesoporous silica nanoparticles in a magnetically stabilized fluidized bed

Wang, Rui; Hu, Yi-Ru; Chen, Guo; Huang, Wei; Liu, Chun-Zhao

doi:10.1016/j.biortech.2012.01.184

Cited by 101 publications

(44 citation statements)

References 28 publications

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“…This lower reaction rate was related to the potential aggregation of the nanoparticles which could reduce substrate accessibility. Wang et al [45] studied phenol degradation by immobilized laccase on magnetic silica nanoparticles, and similar results were observed at pH 7. The rate of phenol conversion for laccase immobilized onto FeO-2206W smNPs (2.01 µM h −1 ) is almost two times higher than that for fsNPs.…”

Section: Biotransformation Of Phenol By Laccase Immobilized Onto Fsnpmentioning

confidence: 49%

“…The immobilized laccase retained 95%, 97%, and 100% of its initial activity at pH 5, 6, and 7, respectively, after incubation at room temperature for 24 h. The reusability of the nanobiocatalyst was assessed in consecutive cycles of 24 h. It was observed that phenol transformation was higher than 60% and was maintained constant after four cycles (Figure 2). In other reports, phenol was almost entirely biotransformed in consecutive cycles with laccase immobilized on magnetic mesoporous silica nanoparticles with a dose of enzyme 18 times higher [45]. The immobilized laccase retained 97% of its initial activity after the consecutive batch treatments of phenol with magnetic separation.…”

Section: Sequential Batch Biotransformation Of Phenol By Laccase Immomentioning

confidence: 99%

“…Due to the remarkable effect of pH on phenol conversion reported in previous works [45,47], the biotransformation of phenol was assessed at different pH levels (5, 6, and 7). The conversion efficiencies and rates are shown in Table 3.…”

Section: Sequential Batch Biotransformation Of Phenol By Laccase Immomentioning

confidence: 99%

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Formulation of Laccase Nanobiocatalysts Based on Ionic and Covalent Interactions for the Enhanced Oxidation of Phenolic Compounds

et al. 2017

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Featured Application: Development and potential use of nanobiocatalysts for the removal of phenolic compounds as well as other related xenobiotics present in industrial wastewaters. Abstract:Oxidative biocatalysis by laccase arises as a promising alternative in the development of advanced oxidation processes for the removal of xenobiotics. The aim of this work is to develop various types of nanobiocatalysts based on laccase immobilized on different superparamagnetic and non-magnetic nanoparticles to improve the stability of the biocatalysts. Several techniques of enzyme immobilization were evaluated based on ionic exchange and covalent bonding. The highest yields of laccase immobilization were achieved for the covalent laccase nanoconjugates of silica-coated magnetic nanoparticles (2.66 U mg −1 NPs), formed by the covalent attachment of the enzyme between the aldehyde groups of the glutaraldehyde-functionalized nanoparticle and the amino groups of the enzyme. Moreover, its application in the biotransformation of phenol as a model recalcitrant compound was tested at different pH and successfully achieved at pH 6 for 24 h. A sequential batch operation was carried out, with complete recovery of the nanobiocatalyst and minimal deactivation of the enzyme after four cycles of phenol oxidation. The major drawback associated with the use of the nanoparticles relies on the energy consumption required for their production and the use of chemicals, that account for a major contribution in the normalized index of 5.28 × 10 −3 . The reduction of cyclohexane (used in the synthesis of silica-coated magnetic nanoparticles) led to a significant lower index (3.62 × 10 −3 ); however, the immobilization was negatively affected, which discouraged this alternative.

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Section: Biotransformation Of Phenol By Laccase Immobilized Onto Fsnpmentioning

confidence: 49%

Section: Sequential Batch Biotransformation Of Phenol By Laccase Immomentioning

confidence: 99%

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Formulation of Laccase Nanobiocatalysts Based on Ionic and Covalent Interactions for the Enhanced Oxidation of Phenolic Compounds

et al. 2017

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“…Laccases immobilized onto silica nanoparticles have been used to remove bisphenol from secondary effluent in a municipal wastewater treatment plant [96] as well as phenol from coking wastewater [97]. There are many studies on the synthesis and comparison of SENs with free enzymes; however, SEN applications for wastewater treatment are still in the rational design phase [98,99].…”

Section: Biocatalysts In Effluent Treatmentmentioning

confidence: 99%

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

et al. 2017

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Abstract:The establishment of a bioeconomy era requires not only a change of production pattern, but also a deep modernization of the production processes through the implementation of novel methodologies in current industrial units, where waste materials and byproducts can be utilized as starting materials in the production of commodities such as biofuels and other high added value chemicals. The utilization of renewable raw resources and residues from the agro-industries, and their exploitation through various uses and applications through technologies, particularly solid-state fermentation (SSF), are the main focus of this review. The advocacy for biocatalysis in green chemistry and the environmental benefits of bioproduction are very clear, although this kind of industrial process is still an exception and not the rule. Potential and industrial products, such as biocatalysts, animal feed, fermentation medium, biofuels (biodiesel, lignocelulose ethanol, CH 4 , and H 2 ), pharmaceuticals and chemicals are dealt with in this paper. The focus is the utilization of renewable resources and the important role of enzymatic process to support a sustainable green chemical industry.

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“…Intimate contact of aerobes with anaerobes may reduce the accumulation of toxic intermediates as the aerobes would achieve an in situ removal of these anaerobic metabolites [12,13] . A simultaneous anaerobic/aerobic treatment methodology using granular sludge was recently described (example for the granule in the EGSB reactor) [14][15][16][17] . Faculative bacteria have been shown to be predominant in the peripheral layer while the methanogens are preferentially biodegradation of the recalcitrant xenobiotic compounds but also in the simultaneous removal of toxic organic compounds and NH 3 -N in the coking wastewater.…”

Section: Introductionmentioning

confidence: 99%

Granulation for Coking Wastewater Treatment in a Coupled Anaerobic-Aerobic Reactor

Chen¹,

Bing-nan

2018

E3S Web Conf.

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Abstract：A coupled anaerobic-aerobic granular bio-film reactor was employed with two operation stages: StageⅠ, granular sludge was formed from digestion sludge using brewery wastewater, and Stage Ⅱ, granular sludge was acclimatized using coking wastewater. Two oxygenation methods (i.e. A and B) were employed to acclimatize the granules. For method A, dissolved O2 was supplied through a continuous oxygenation way of 800-15000mlmin -1 . And for method B, dissolved O2 was supplied of 800-15000mlmin -1 18-12 times at 20-60min intervals, 1h each time. The experimental results showed that granules could quickly form in 10d in the EGSB reactor seeded with digestion sludge and little loose granules lack of nutrition, and it was the key factor for granules forming to add little loose granules. It took only about 6 months for granules acclimation using coking wastewater. Both oxygenation methods could run well when acclimatizing the granules. However, method A could have comparatively high and stable operation effect. The actual coking wastewater had distinct inhibition effect on the granules, but the supplement of some oxygen could promote the recovery of SMA, and NaHCO3 supplement could also weaken the inhibition effect of the CWW. Method A had more strongly activity recovery ability than method B.

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Enhanced phenol degradation in coking wastewater by immobilized laccase on magnetic mesoporous silica nanoparticles in a magnetically stabilized fluidized bed

Cited by 101 publications

References 28 publications

Formulation of Laccase Nanobiocatalysts Based on Ionic and Covalent Interactions for the Enhanced Oxidation of Phenolic Compounds

Formulation of Laccase Nanobiocatalysts Based on Ionic and Covalent Interactions for the Enhanced Oxidation of Phenolic Compounds

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Granulation for Coking Wastewater Treatment in a Coupled Anaerobic-Aerobic Reactor

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