Enhanced photoacoustic response with plasmonic nanoparticle-templated microbubbles

Dove, Jacob D.; Murray, Todd W.; Borden, Mark A.

doi:10.1039/c3sm51690c

Cited by 48 publications

(55 citation statements)

References 39 publications

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“…We do note that a small amount of bubble growth was observed at later times (several seconds) most likely due to gas efflux and influx of the core [26,27]. We have previously shown that nanoparticle-loaded microbubble oscillations can be induced by a pulsed laser source, and that the photoacoustic response from such microbubble populations exceeds that from free nanoparticles at the same nanoparticle concentration [22,28]. The microbubbles produced post vaporization remain optically active, indicating that the nanoparticles are not purged during the explosive vaporization of the droplet core.…”

Section: Resultsmentioning

confidence: 99%

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Engineering optically triggered droplets for photoacoustic imaging and therapy

Dove

Mountford

Murray

et al. 2014

Biomed. Opt. Express

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Liquid perfluorocarbon (PFC) droplets incorporating optical absorbers can be vaporized through photothermal heating using a pulsed laser source. Here, we report on the effect of droplet core material on the optical fluence required to produce droplet vaporization. We fabricate gold nanoparticle templated microbubbles filled with various PFC gases (C 3 F 8 , C 4 F 10, and C 5 F 12 ) and apply pressure to condense them into droplets. The core material is found to have a strong effect on the threshold optical fluence, with lower boiling point droplets allowing for vaporization at lower laser fluence. The impact of droplet size on vaporization threshold is discussed, as well as a proposed mechanism for the relatively broad distribution of vaporization thresholds observed within a droplet population with the same core material. We propose that the control of optical vaporization threshold enabled by engineering the droplet core may find application in contrast enhanced photoacoustic imaging and therapy.

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Section: Resultsmentioning

confidence: 99%

“…Nanoparticles were attached to the microbubble shell following a previously described avidin-biotin conjugation scheme [22]. First, the bubbles were sized to estimate the number of binding sites.…”

Section: Microbubble Fabricationmentioning

confidence: 99%

Engineering optically triggered droplets for photoacoustic imaging and therapy

Dove

Mountford

Murray

et al. 2014

Biomed. Opt. Express

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“…The expansion of the gas core then occurs through thermal effects. 124,148 Thus, heat rather than light is generating or driving the bubbles, which in turn generates or scatters the acoustic waves for imaging purposes.…”

Section: Optical Excitationmentioning

confidence: 99%

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

et al. 2016

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Besides their use as contrast agents for ultrasound imaging, microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes. V C 2016 AIP Publishing LLC.

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“…The best choice for the wavelength will therefore also depend on the stage of development of the considered agent. Practically, if the bubble are equipped with the right fluorophores, the use of light can have a direct impact on the bubbles; the bubble expansion occurs through thermal effects 112,127 . Thus, heat rather than light is generating or driving the bubbles, which in turn generates or scatters the acoustic waves for imaging purposes.…”

Section: Excitation Of the Microbubbles Optical Excitationmentioning

confidence: 99%

“…However this technology is inherently limited by the amount of energy required to vaporize the surrounding liquid. To overcome this limitation, stabilized microbubbles enhanced with an absorbing material are proposed 112,127,302,317 . Thus, provided they can be functionalized to increase their optical absorption characteristics, the ability of such microbubbles to produce strong acoustic signals can similarly be exploited in PA imaging.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound contrast agents: bubbles drops and particles

Lajoinie¹

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The research described in this thesis is part of the research program NanoNextNL, a micro and nanotechnology consortium of the Government of the Netherlands and 130 partners. This thesis was carried out at the Physics of Fluids group of the Faculty of Science and Technology of the University of Twente. Cover design:As a representation of a crucial goal of this work, the cover depicts a confocal microscopy image showing cells selectively porated by loaded microbubbles upon ultrasound exposure. The porated cells take up a blue fluorescent probe. By Ine Lentacker, Ine De Cock and Guillaume Lajoinie. Guide through the thesisIn this thesis, we investigate different types of innovative agents, designed for biomedical use in a context of molecular imaging. In Chapter 1, we review the agents that arouse the interest of the scientific community, most of them at the research stage of development, together with the experimental methods that can be used to study their interaction with cells. Such in vitro investigations are necessary to test on short scales the impact of the various agents on biological structures.We separate the agents in three main categories. The first group consists of stabilized microbubbles, usually by means of phospholipids that fill up the interfacial area between the gas core and the surrounding liquid (water). Such bubbles are clinically used for 40 years as contrast agents for ultrasound owing to their unique resonance behavior. When irradiated with an ultrasound pulse, a bubble experiences volumetric oscillations, that reemit an ultrasound wave back to the transducer. This process makes them unchallenged ultrasound scatterers. The role of these stable microbubbles in new biomedical research directions is discussed in Chapters 2 to 9.Phospholipid coatings were developed in the last decade to do much more than just prevent bubble dissolution. They are now a crucial tool to enrich the microbubbles with specific markers or drug payloads. In Chapter 2, we investigate the behavior of this coating when the microbubbles are subjected to an ultrasound field. We show that the coating is shed away from the microbubble, which is of major interest for molecular imaging combined with drug delivery using microbubbles. We pursue the idea in Chapter 3 where we investigate how a bubble payload, here liposomes labeled with a fluorescent dye, is released under ultrasound exposure. In Chapter 4, we link these direct observations of shedding of the previous Chapters to the subsequent dissolution of the microbubbles, no longer protected by a phospholipid shell. This dissolution, visible in the ultrasound scatter spectrum can be used to remotely quantify the effect of the microbubbles. In support of these observations, we dedicate the next Chapter (Chapter 5) to physically understand the mechanisms underlying the behavior of the phospholipid coating. The application of these findings in practice requires the production a sufficiently high production rate and with great control of such microbubbles. In Chapter 6, we th...

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Enhanced photoacoustic response with plasmonic nanoparticle-templated microbubbles

Cited by 48 publications

References 39 publications

Engineering optically triggered droplets for photoacoustic imaging and therapy

Engineering optically triggered droplets for photoacoustic imaging and therapy

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

Ultrasound contrast agents: bubbles drops and particles

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