Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation

Putalun, Waraporn; Udomsin, Orapin; Yusakul, Gorawit; Juengwatanatrakul, Thaweesak; Sakamoto, Seiichi; Tanaka, Hiroyuki

doi:10.1007/s10529-010-0202-3

Cited by 59 publications

(25 citation statements)

References 14 publications

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“…Some species, mainly Drosera rotundifolia L., D. intermedia Hayne and D. anglica Huds., are commonly used in traditional medicine as the therapy of respiratory tract (Paper et al, 2005;Fukushima et al, 2009). Recently, several bioactive compounds from sundew leaves and roots including flavonoids and quinones have been found (Hook, 2001;Marczak et al, 2005;Putalun et al, 2010). These compounds can be considered as very important metabolites (Milella et al, 2011;Padula et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Rejthar¹,

Viehmannová

Čepková³

et al. 2014

Emir. J. Food Agric

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This study was aimed to optimize in vitro propagation of Drosera intermedia for commercial and conservation purposes. The effect of concentration of MS nutrients (⅛ MS, ¼ MS, ½ MS and, MS), various pH (3.7-7.7), sucrose concentration (10-40 g l -1 ) and cytokinins (0.1-3 mg l -1 ), namely BA (N6-benzyladenine), kinetin and zeatin were evaluated. After 60 days of shoot cultivation, growth and developmental characteristics (plant height, number of shoots per explant, diameter of rosette, number of roots per explant, length of roots) were recorded. No significant differences were found for various levels of pH and sucrose. On the contrary, plant height was negatively influenced by an increase of nutrients in the medium. The plants on 1/8 MS medium were significantly taller, and displayed higher proliferation capacity compared with those cultivated on full-strength MS medium. Shoot multiplication and growth was suppressed by supplementation of BA and kinetin, regardless of concentration used. Zeatin at the lowest concentration (0.1 mg l -1 ) provided the best results for shoot proliferation of all 26 treatments and can be recommended for micropropagation of D. intermedia.

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Section: Introductionmentioning

confidence: 99%

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Rejthar¹,

Viehmannová

Čepková³

et al. 2014

Emir. J. Food Agric

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“…The addition of a part of the pathogen cellular wall induces a defensive response and, as a result, the naphthoquinone synthesis occurs (Juniper et al 1989). Putalun et al (2010) studied the influence of methyl jasmonate, yeast extract and chitosan. In their study, the yeast extract was found to enhance the production of plumbagin in the roots of D. burmanii by 3.5-fold when compared to the control plants.…”

Section: Elicitation Of Secondary Metabolitesmentioning

confidence: 99%

In vitro cultures of carnivorous plants from the Drosera and Dionaea genus for the production of biologically active secondary metabolites

Banasiuk¹,

Kawiak²,

Królicka³

2012

bta

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Carnivorous plants belong to endangered species. Due to agricultural development, natural populations of these plants are diminishing. The herbal and ornamental value of these species has also led to their over-collection.The Drosera genus is a natural source of pharmacologically important compounds (e.g. naphthoquinones, flavonoids, anthocyanins, phenolic compounds) used as substrates in the production of pharmaceuticals. Droserae herba has been in use as an expectorant, diuretic and antispasmodic agent. In recent years, the bacteriostatic and antitumour activity of Drosera extracts has been reported. Carnivorous plants have become an important ornamental element in botanical garden collections. This fact, as well as the low propagation rate in their natural environment, is the reason for the in vitro propagation of carnivorous plants. From a single plant cultivated in vitro many genetically identical clonal lines can be obtained through vegetative propagation. This technique allows for the increase in the propagation rate of valuable plant material. Additionally, the use of biotic and abiotic elicitors increases the synthesis of pharmacologically active compounds (bactericidal, bacteriostatic activity and cytotoxic activity). Elicitors play a significant role in the production of secondary metabolites. They induce defense responses in plants, which leads to the accumulation of secondary metabolites. In some cases, compounds not synthesized normally by plants in their natural environment are produced upon elicitation. Elicitors induce the biosynthesis of enzymes which take part in the production of secondary metabolites.

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“…Moreover, high regeneration potential in in vitro conditions has been shown for several members of Droseraceae (Bobák et al 1989(Bobák et al , 1995Š amaj et al 1999;Kawiak et al 2003;Jayaram and Prasad 2008;Grevenstuk et al 2010;Rejthar et al 2014). Since protocols are available for production of valuable metabolites from in vitro cultures, sundew is attractive and promising for biotechnology (Putalun et al 2010). Previously, Król-icka et al (2010) have succeeded in increasing naphtoquinone production in Agrobacterium rhizogenes induced teratomas of in vitro grown Drosera capensis.…”

Section: Introductionmentioning

confidence: 99%

Transformation of sundew: pitfalls and promises

Blehová

Švubová

Lukačová

et al. 2014

Plant Cell Tiss Organ Cult

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Functional studies and exploitation of pharmacological potential of sundews (Droseraceae) require efficient and reliable transformation protocol. However, the routine applicability of available procedures appears limited and transformation of sundew remains a difficult task.Here we report on in-depth evaluation of the previously published method and bring some modifications. We suggest addition of glutathione in cultivation media and protection from light for suppression of necrosis. Further we show that sundew leaf extracts exert antimicrobial activity, therefore we recommend selection of tolerant Agrobacterium strain. At the same time we conclude that A. rhizogenes is not suitable for sundew transformation since produces only albino plants. To support regeneration of transformed shoots we propose to use GFP as a vital marker for early identification and removal of escapes and chimeras. The modified protocol we provide enables a routine and reliable selection of stably transformed sundew plants.

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Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation

Cited by 59 publications

References 14 publications

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

In vitro cultures of carnivorous plants from the Drosera and Dionaea genus for the production of biologically active secondary metabolites

Transformation of sundew: pitfalls and promises

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