Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase

Yokoo, Toshinori; Matsumoto, Ken’ichiro; Ooba, Takashi; Morimoto, Kenjiro; Taguchi, Seiichi

doi:10.1080/09168451.2014.1002448

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Point mutations Q47L and T173S in PhaB have previously been shown to increase activity and poly-3-HB accumulation in Corynebacterium glutamicum ( Matsumoto et al, 2013 ) and in tobacco ( Yokoo et al, 2015 ). Both point mutations were introduced separately into PhaB (plasmids p_ phaB _L and p_ phaB _S) and a double mutant (plasmid p_ phaB _LS) was produced.…”

Section: Resultsmentioning

confidence: 99%

“…In one mutant a glutamine at position 47 was changed to leucine (Q47L) and in the other mutant a threonine at position 173 was changed to serine (T173S). Both mutated enzymes showed increased activity and poly-3-hydroxybutyrate accumulation when heterologously expressed in Corynebacterium glutamicum ( Matsumoto et al, 2013 ) and in tobacco ( Yokoo et al, 2015 ). It was hypothesised that introduction of these point mutations in phaB and expression in C. saccharoperbutylacetonicum should analogously lead to higher metabolic flux toward ( R )-1,3-BDO.…”

Section: Discussionmentioning

confidence: 99%

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Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol

Grosse-Honebrink

Little

Bean

et al. 2021

Front. Bioeng. Biotechnol.

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Chirally pure (R)-1,3-butanediol ((R)-1,3-BDO) is a valuable intermediate for the production of fragrances, pheromones, insecticides and antibiotics. Biotechnological production results in superior enantiomeric excess over chemical production and is therefore the preferred production route. In this study (R)-1,3-BDO was produced in the industrially important whole cell biocatalyst Clostridium saccharoperbutylacetonicum through expression of the enantio-specific phaB gene from Cupriavidus necator. The heterologous pathway was optimised in three ways: at the transcriptional level choosing strongly expressed promoters and comparing plasmid borne with chromosomal gene expression, at the translational level by optimising the codon usage of the gene to fit the inherent codon adaptation index of C. saccharoperbutylacetonicum, and at the enzyme level by introducing point mutations which led to increased enzymatic activity. The resulting whole cell catalyst produced up to 20 mM (1.8 g/l) (R)-1,3-BDO in non-optimised batch fermentation which is a promising starting position for economical production of this chiral chemical.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%