Enhanced pre-pubertal nutrition upregulates mitochondrial function in testes and sperm of post-pubertal Holstein bulls

Johnson, Chinju; Dance, Alysha; Kovalchuk, Igor; Kastelic, John P.; Thundathil, Jacob C.

doi:10.1038/s41598-020-59067-3

Cited by 12 publications

(5 citation statements)

References 49 publications

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“…Similar to cows, paternal metabolic status significantly influences semen quality. Enhanced pre-pubertal nutrition elevates the percentage of progressively motile and upregulates mitochondrial function in sperm of post-pubertal dairy bulls ( Johnson et al, 2020 ). In contrast, low planes of nutrition of young Holstein-Friesian bulls resulted in the retarded onset of puberty ( Byrne et al, 2018 ).…”

Section: The Known Non-genetic Parental Influences In Dairy Cattlementioning

confidence: 99%

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Sirard

2020

Front. Genet.

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The bovine represents an important agriculture species and dairy breeds have experienced intense genetic selection over the last decades. The selection of breeders focused initially on milk production, but now includes feed efficiency, health, and fertility, although these traits show lower heritability. The non-genetic paternal and maternal effects on the next generation represent a new research topic that is part of epigenetics. The evidence for embryo programming from both parents is increasing. Both oocytes and spermatozoa carry methylation marks, histones modifications, small RNAs, and chromatin state variations. These epigenetic modifications may remain active in the early zygote and influence the embryonic period and beyond. In this paper, we review parental non-genetic effects retained in gametes on early embryo development of dairy cows, with emphasis on parental age (around puberty), the metabolism of the mother at the time of conception and in vitro culture (IVC) conditions. In our recent findings, transcriptomic signatures and DNA methylation patterns of blastocysts and gametes originating from various parental and IVC conditions revealed surprisingly similar results. Embryos from all these experiments displayed a metabolic signature that could be described as an “economy” mode where protein synthesis is reduced, mitochondria are considered less functional. In the absence of any significant phenotype, these results indicated a possible similar adaptation of the embryo to younger parental age, post-partum metabolic status and IVC conditions mediated by epigenetic factors.

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Section: The Known Non-genetic Parental Influences In Dairy Cattlementioning

confidence: 99%

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Sirard

2020

Front. Genet.

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“…In line with these observations, we observed that the sperm motility was significantly lower in low-fertile bulls compared to high-fertile bulls. Oxidative phosphorylation is an important pathway of energy production in mammalian spermatozoa (61)(62)(63)(64)(65). We observed that 24 genes associated with the oxidative phosphorylation pathway were downregulated in low-fertile bull spermatozoa.…”

Section: Discussionmentioning

confidence: 83%

Transcriptomic Profiling of Buffalo Spermatozoa Reveals Dysregulation of Functionally Relevant mRNAs in Low-Fertile Bulls

et al. 2021

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Although, it is known that spermatozoa harbor a variety of RNAs that may influence embryonic development, little is understood about sperm transcriptomic differences in relation to fertility, especially in buffaloes. In the present study, we compared the differences in sperm functional attributes and transcriptomic profile between high- and low-fertile buffalo bulls. Sperm membrane and acrosomal integrity were lower (P < 0.05), while protamine deficiency and lipid peroxidation were higher (P < 0.05) in low- compared to high-fertile bulls. Transcriptomic analysis using mRNA microarray technology detected a total of 51,282 transcripts in buffalo spermatozoa, of which 4,050 transcripts were differentially expressed, and 709 transcripts were found to be significantly dysregulated (P < 0.05 and fold change >1) between high- and low-fertile bulls. Majority of the dysregulated transcripts were related to binding activity, transcription, translation, and metabolic processes with primary localization in the cell nucleus, nucleoplasm, and in cytosol. Pathways related to MAPK signaling, ribosome pathway, and oxidative phosphorylation were dysregulated in low-fertile bull spermatozoa. Using bioinformatics analysis, we observed that several genes related to sperm functional attributes were significantly downregulated in low-fertile bull spermatozoa. Validation of the results of microarray analysis was carried out using real-time qPCR expression analysis of selected genes (YBX1, ORAI3, and TFAP2C). The relative expression of these genes followed the same trend in both the techniques. Collectively, this is the first study to report the transcriptomic profile of buffalo spermatozoa and to demonstrate the dysregulation of functionally relevant transcripts in low-fertile bull spermatozoa. The results of the present study open up new avenues for understanding the etiology for poor fertility in buffalo bulls and to identify fertility biomarkers.

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“…Furthermore, a series of studies have shown that sperm motility and sperm progressive motility signify the function of mitochondria and energy status, which have been associated with fertility. 25,26 Studies have also shown that the VCL reflects the high-energy state of sperm and hyperactivated motility, which are essential for successful fertilization. 27,28 However, to a large extent, the short-term and cumulative effects of a HFD on these sperm parameters are controversial.…”

Section: Discussionmentioning

confidence: 99%

The time-dependent adverse effects of a high-fat diet on sperm parameters

Qi¹,

Guan²,

Zhang³

et al. 2023

Adv Clin Exp Med

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Background. Studies indicate a relationship between a high-fat diet (HFD) and sperm quality. However, the time-dependent adverse effects of a HFD on sperm parameters and the underlying mechanisms remain unclear.Objectives. The present study was designed to determine the effects of a HFD on sperm quality at various time points in order to assess whether a HFD causes cumulative damage to sperm. Materials and methods.Male C57BL/6 mice were fed a normal diet (the ND group) or a HFD (the HFD group) for 16, 30 or 42 weeks (n = 6 for each group). Body weight, lipid profile, sperm parameters, testicular morphology, and testicular oxidative stress levels were evaluated alongside the proliferation, DNA damage and rate of germ cell apoptosis.Results. Sperm quality was reduced in HFD-fed animals in a time-dependent manner, which was demonstrated by a decline in sperm density, motility and progressive motility. Further analysis showed a progressive deterioration of the testicular histoarchitecture of HFD-fed mice, which was accompanied by a decrease in DEAD-box helicase 4 (DDX4) expression and superoxide dismutase (SOD) levels, increased malondialdehyde (MDA) levels and gamma-H2A histone family member X (γ-H2AX) expression, and increased apoptosis of germ cells.Conclusions. These findings demonstrate that a HFD exerted adverse effects on sperm quality, and the deteriorating effect was progressive with long-term feeding. The inhibited proliferation and apoptosis of germ cells, and the increased oxidative stress levels and DNA damage may be the underlying mechanisms.

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Enhanced pre-pubertal nutrition upregulates mitochondrial function in testes and sperm of post-pubertal Holstein bulls

Cited by 12 publications

References 49 publications

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Transcriptomic Profiling of Buffalo Spermatozoa Reveals Dysregulation of Functionally Relevant mRNAs in Low-Fertile Bulls

The time-dependent adverse effects of a high-fat diet on sperm parameters

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