Enhanced production of ligninolytic enzymes and decolorization of molasses distillery wastewater by fungi under solid state fermentation

Abstract

Selected isolates of fungi were grown on wheat straw and corncob in the presence of different moistening agents such as water, molasses, potato dextrose broth and distillery effluent. All the fungal isolates responded differently with respect to growth and ligninolytic enzyme production. Fungal growth on different substrates was checked by calculating ergosterol content, which varied widely within a single species when grown on different substrates. The maximum laccase production was obtained for Aspergillus flavus TERI DB9 grown on wheat straw with molasses. For manganese peroxidase, highest production was in Aspergillus niger TERI DB20 grown on corncob with effluent. Among the two isolates positive for lignin peroxidase, the highest production was in Fusarium verticillioides ITCC 6140. This immobilized fungal biomass was then used for decolorization of effluent from a cane molasses based distillery. Maximum decolorization (86.33%) was achieved in Pleurotus ostreatus (Florida) Eger EM 1303 immobilized on corncob with molasses in a period of 28 days.

Tyrosinase and peroxidase production by Rhizopus oryzae strain ENHE obtained from pentachlorophenol‐contaminated soil

“…and Aspergillus fumigatus has been reported. 39 Pant and Adholeya 40 reported the production of LiP by Fusarium verticillioides and MnP by Aspergillus flavus, both fungi cultured in solid state fermentation. In the present study, extracellular LiP activity of R. oryzae ENHE was found in cultures with and without PCP, though this activity was higher when PCP was added to the culture medium.…”

Simultaneous Laccase Production and Color Removal by Culturing Fungus Pycnoporus sp. SYBC-L3 in a Textile Wastewater Effluent Supplemented with a Lignocellulosic Waste Phragmites australis

“…Most previous studies focused on the use of crude or pure laccases for the treatment of textile effluent (Wu et al 2002) or synthetic dyes (Li et al 2009). Pant et al studied solid state fermentation by fungi using distillery wastewater as a natural medium and achieved laccase production and decolorization (Pant et al 2007 However, reports on using dye-containing effluent as a natural medium for cultivation to obtain a decoloration effect have been rare. To the best of our knowledge, our study marked the first report on using a real textile effluent as the culture medium for simultaneous color removal and laccase production.…”

Decolourization of anaerobically digested and polyaluminium chloride treated distillery spentwash in a fungal stirred tank aerobic reactor

“…They found 75% decolourization with diluted wastewater which reduced to 40% on using undiluted wastewater. In another study, colour removal of 86, 50 and 47% was observed with Pleurotus florida, Penicillium pinophilum and Alternaria gaisen, respectively (Pant and Adholeya 2007b). Kaushik and Thakur (2009) isolated five different fungi from distillery mill site and the two isolates having higher capabilities to remove colour were identified as Emericella nidulans and Neurospora intermedia, respectively.…”