Enhanced production of pectinase by Saccharomyces cerevisiae isolate using fruit and agro-industrial wastes: Its application in fruit and fiber processing

Characterization and molecular modeling of polygalacturonase isoforms from Saccharomyces cerevisiae

“…stimulated enzyme activity by 86.7, 96.4, and 81.5%. These results were in agreement with previous findings by Vijayakumar et al (2016), whereas less inhibition was observed with Mg 2? , Zn 2?…”

“…Considering the fact that pectinases differ in molecular sizes, pH, temperature optima, and other characteristics from different sources of microorganisms as well as their prospective applications in various fields, it is important to understand nature and properties of these enzymes for efficient and effective usage. We have previously reported the production, isolation, and purification of low-temperatureactive polygalacturonase isoforms by Saccharomyces cerevisiae strain PVK4 using citrus pectin as substrate (Vijayakumar et al 2015(Vijayakumar et al , 2016. In the present work, the characterizations of purified polygalacturonase isoforms from S. cerevisiae and substrate specificities of the enzymes with pectic and non-pectic polysaccharides were studied.…”

“…The yeast Saccharomyces cerevisiae strain PVK4 was previously isolated from fruit waste and low-temperatureactive polygalacturonase isoforms were characterized (Vijayakumar et al 2015(Vijayakumar et al , 2016. In the present study, the same isoforms were used for further characterization.…”

Use of Agro-Industrial Wastes for the Production of a Wild Yeast Enzyme with Disintegration Activity on Plant Tissues

“…Yeast extract, and Fe +2 had insignificant effect on enzyme productivity [51]. Poondla and coauthors studied the influence of different metal ions in the production of PGase by Saccharomyces cerevisiae, the production of enzymes increased considerably with MnSO4> KH2PO4> ZnSO4> CaCl2> MgSO4> CoCl2 [9].…”

“…Consequently, the accumulation of great quantities of organic residues represents a risk to health and environment [4]. The production of microbial enzymes by a biotechnological process, using agricultural wastes in the culture medium, represents a suitable alternative for re-valorization of such a type of residues, reducing environmental pollution and generating products with high-added value, resulting in economic and ecological advantages [5][6][7][8][9].…”