Enhanced Protein Adsorption Capacity of Macroporous Pectin Particles with High Specific Surface Area and an Interconnected Pore Network

Abstract

There has been much interest in developing protein adsorbents using nanostructured particles, which can be engineered porous materials with fine control of the surface and pore structures. A significant challenge in designing porous adsorbents is the high percentage of available binding sites in the pores owing to their large surface areas and interconnected pore networks. In this study, continuing the idea of using porous materials derived from natural polymers toward the goal of sustainable development, porous pectin particles are reported. The template-assisted spray drying method using calcium carbonate (CaCO 3 ) as a template for pore formation was applied to prepare porous pectin particles. The specific surface area was controlled from 177.0 to 222.3 m 2 g −1 by adjusting the CaCO 3 concentration. In addition, the effects of a macroporous structure, the specific surface area, and an interconnected pore network on the protein (lysozyme) adsorption capacity and adsorption mechanism were investigated. All porous pectin particles performed rapid adsorption (∼65% total capacity within 5 min) and high adsorption capacity, increasing from 1543 to the highest value of 2621 mg g −1 . The results are attributed to the high percentage of available binding sites located in the macropores owing to their large surface areas and interconnected pore networks. The macroporous particles obtained in this study showed a higher adsorption capacity (2621 mg g −1 ) for lysozyme than other adsorbents. Moreover, the rapid uptake and high performance of this material show its potential as an advanced adsorbent for various macromolecules in the food and pharmaceutical fields.

Effects of Solvent Polarity on Nanostructure Formation of Spray-Dried TEMPO-Oxidized Cellulose Nanofiber Particles

“…The adsorption isotherm type was determined using a modified version of the previously reported batch equilibrium method. , Adsorption experiments were performed at room temperature (25 °C) with TOCN particles (20 mg/mL) dispersed in the lysozyme solution (50 mL). The initial lysozyme concentration was adjusted to 0.6 mg/mL, and the pH was adjusted to 7.0 with phosphate buffer.…”

Using magnetite/zirconium-comodified attapulgite as a novel phosphorus (P) sorbent for the efficient removal of P and the adsorption mechanism allowing this effect

“…An automatic specific surface and porosity analyzer is used to obtain the physical property parameters, such as the specific surface area, pore volume and pore size distribution. The specific surface area of Fe/ZrATP (138.256 m 2 /g) is obviously greater than that of ATP (100.37 m 2 /g), which may be because the metal elements Fe and Zr are not only distributed on the surface of ATP but also enter the pores of the concavite in small amounts, increasing the layer spacing of the material and forming a bilayer structure with an increased surface area (Nguyen et al 2022). Then, the specific surface area of Fe/ZrATP increases again due to the uniform load of Fe 3 O 4 , which yields a rough composite surface, as observed from the SEM images in Fig.…”

Designing the Macroporous Structure of Three-Way Catalyst Particles: The Influence of Template Concentration on Framework Thickness and Mass Transfer

“…In this study, the macroporous TWC particles were facilely synthesized by a template-assisted aerosol process. The template-assisted aerosol process − has been widely employed to produce the macroporous structure of various materials, such as carbon, , silica, , metal and metal oxides, , and natural polymers. , Therefore, the aerosol process may be potentially effective in preparing the macroporous TWC particles with the assistance of lab-made polystyrene latex (PSL) as a template. However, no previous study has reported the particle formation mechanism of macroporous TWC particles via the aerosol route as well as the influence of PSL concentration on the framework thickness between macropores.…”