Enhanced representation of HL-A antigens on human lymphocytes after mitogenesis induced by phytohemagglutinin or Epstein-Barr virus.

McCune, Joseph M.; Humphreys, Robert E.; Yocum, R. Rogers; Strominger, Jack L.

doi:10.1073/pnas.72.8.3206

Cited by 57 publications

(21 citation statements)

References 18 publications

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“…Washed platelets (blood groups O, Rh +) were suspended at 10 ~° platelets/ml and diluted 1:2 serially in dextrose-gelatin-veronal buffer (DGV). 3 Aliquots of platelets (25 pl) were incubated for i h at 4°C with 25-~I aliquots of(a) 1:50 diluted anti-p23,30 or (b) 1:25 diluted anti-~ microglobulin serum in microtiter plates following a previously described absorption technique (13). After absorption, the plates were centrifuged and 5-~I aliquots of the supernate were assayed for the presence of cytotoxic antibodies against peripheral blood lymphocytes (PBL's).…”

Section: Separation Of B Lymphocytes By Adherence In a Microtitermentioning

confidence: 99%

Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen.

Humphreys¹,

McCune²,

Chess³

et al. 1976

The Journal of Experimental Medicine

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204

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Lymphocytes bear many types of surface molecules, some of which are restricted to subpopulations of lymphocytes and may be necessary for their specialized immune functions. Examples of such antigens include: the Ia antigens; cell surface immunoglobulin; the Fc receptor, complement (C) receptors, and Ly-4 all present predominantly on B lymphocytes and Thy (theta) antigen; and sheep erythrocyte receptor, and the Ly-1, 2, and 3 antigens all present on T cells. H antigens (H-2 or HL-A) and ~-microglobulin are found on all populations on lymphocytes. Because a non-H-2, B-lymphocyte-specific antigen had been serologically defined on the murine lymphoblast line L1210 (reference i and footnote 1) and because a similar human, non-HL-A antigen could be present on the human B-lymphoblast line IM-1, from which the isolation of the HL-A antigen was in progress, attention has been paid to the "contaminant" molecules of the HL-A antigen preparations/ Several cell surface antigens have been identified, one of which (p23,30) is similar to Ia antigens which have been described on mouse B lymphocytes. The purification of that antigen, its distribution on distinct subsets of human lymphocytes, and the properties of heteroantisera to p23,30 in lymphocyte functional assays are described in this and the accompanying paper (3).

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Section: Separation Of B Lymphocytes By Adherence In a Microtitermentioning

confidence: 99%

Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen.

Humphreys¹,

McCune²,

Chess³

et al. 1976

The Journal of Experimental Medicine

Self Cite

204

View full text Add to dashboard Cite

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“…This difficulty has been overcome to a certain extent by the development of monoclonal antibodies against single antigenic specificities and by the use of apparently homozygous cell lines derived from offspring of consanguinous marriages (2,4,38,43,44). An alternative method of generating cell lines that express single, defined HLA antigens is to isolate HLA genes and introduce them into cells in culture that do not otherwise express HLA antigens.…”

mentioning

confidence: 99%

High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.

DiMaio¹,

Corbin²,

Sibley³

et al. 1984

Mol. Cell. Biol.

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A gene encoding the heavy chain of an HLA human histocompatibility antigen was isolated from a library of human DNA by recombination and selection in vivo. After insertion into a bovine papillomavirus (BPV) DNA expression vector, the gene was introduced into cultured mouse cells. Cells transformed with the HLA-BPV plasmids did not appear to contain extrachromosomal viral DNA, whereas BPV recombinants usually replicated as plasmids in transformed cell lines. Large amounts of HLA RNA were produced by the transformed cells, and the rate of synthesis of human heavy chain was several-fold higher than in the JY cell line, a well-characterized human lymphoblastoid cell line which expresses high levels of surface HLA antigen. Substantial amounts of human heavy chain accumulated in the transformed cells, and HLA antigen was present at the cell surface. These observations establish the feasibility of using BPV vectors to study the structure and function of HLA antigens and the expression of cloned HLA genes.

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“…This point is especially relevant, since it has been shown by AlcCune et al that the density of HLA antigens expressed on activated human lymphocytes in z&o increases as much as IO-fold after stimulation with I'HA (24). I n addition, specific removal of the LT neutralizing capacity of anti-a-LT serum by absorption on activated, but not on unactivated, human lymphocytes further negates the probability that C'-mediated lysis is due to anti-histocompatibility antibodies present in this serum.…”

Section: Discussionmentioning

confidence: 96%

Identification of membrane-associated lymphotoxin (LT) on mitogen-activated human lymphocytes using heterologous anti-LT antisera in vitro

Hiserodt

Ware

Harris

et al. 1977

Cellular Immunology

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Enhanced representation of HL-A antigens on human lymphocytes after mitogenesis induced by phytohemagglutinin or Epstein-Barr virus.

Cited by 57 publications

References 18 publications

Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen.

Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen.

High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.

Identification of membrane-associated lymphotoxin (LT) on mitogen-activated human lymphocytes using heterologous anti-LT antisera in vitro

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