Enhanced resistance to fatigue and altered calcium handling properties of sarcalumenin knockout mice

Zhao, Xiaoli; Yoshida, Morikatsu; Brotto, Leticia; Takeshima, Hiroshi; Weisleder, Noah; Hirata, Yutaka; Nosek, Thomas M.; Ma, Jianjie; Brotto, Marco

doi:10.1152/physiolgenomics.00020.2005

Cited by 73 publications

(89 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SAR knockout mice exhibit enhanced resistance to fatigue and altered Ca 2+ -handling properties (Zhao et al, 2005). SARdeficient fibres show diminished Ca 2+ -uptake into the SR lumen and enhanced store-operated Ca 2+ -entry appears to be a result of chronic adaptation from SAR ablation .…”

Section: Discussionmentioning

confidence: 99%

Reduced expression of sarcalumenin and related Ca2+-regulatory proteins in aged rat skeletal muscle

O’Connell¹,

Gannon²,

Doran³

et al. 2008

Experimental Gerontology

View full text Add to dashboard Cite

a b s t r a c tIn skeletal muscle, Ca 2+ -cycling through the sarcoplasm regulates the excitation-contraction-relaxation cycle. Since uncoupling between sarcolemmal excitation and fibre contraction may play a key role in the functional decline of aged muscle, this study has evaluated the expression levels of key Ca 2+ -handling proteins in senescent preparations using immunoblotting and confocal microscopy. Sarcalumenin, a major luminal Ca 2+ -binding protein that mediates ion shuttling in the longitudinal sarcoplasmic reticulum, was found to be greatly reduced in aged rat tibialis anterior, gastrocnemius and soleus muscle as compared to adult specimens. Minor sarcolemmal components of Ca 2+ -extrusion, such as the surface Ca 2+ -ATPase and the Na + -Ca 2+ -exchanger, were also diminished in senescent fibres. No major changes were observed for calsequestrin, sarcoplasmic reticulum Ca 2+ -ATPase and the ryanodine receptor Ca 2+ -release channel. In contrast, the age-dependent reduction in the a 1S -subunit of the dihydropryridine receptor was confirmed. Hence, this report has shown that downstream from the well-established defect in coupling between the t-tubular voltage sensor and the junctional Ca 2+ -release channel complex, additional age-related alterations exist in the expression of essential Ca 2+ -handling proteins. This may trigger abnormal luminal Ca 2+ -buffering and/or decreased plasmalemmal Ca 2+ -removal, which could exacerbate impaired signaling or disturbed intracellular ion balance in aged fibres, thereby causing contractile weakness.

show abstract

Section: Discussionmentioning

confidence: 99%

Reduced expression of sarcalumenin and related Ca2+-regulatory proteins in aged rat skeletal muscle

O’Connell¹,

Gannon²,

Doran³

et al. 2008

Experimental Gerontology

View full text Add to dashboard Cite

show abstract

“…coupling, a finding that is also mirrored in aged skeletal muscles, which have an ϳ50% reduction in the protein content of MG29 (77,78). In contrast, improved muscle performance in an animal model where the MG29 level was up-regulated has been reported (79). Therefore, the up-regulation of Mg29 along with the up-regulation of other muscle performance genes helps explain the increased contractile performance of SOL muscles from cKO mice.…”

Section: Discussionmentioning

confidence: 93%

Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age

Gorski

Huffman

Vallejo³

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Conditional deletion of Mbtps1 (cKO) protease in bone osteocytes leads to an age-related increase in mass (12%) and in contractile force (30%) in adult slow twitch soleus muscles (SOL) with no effect on fast twitch extensor digitorum longus muscles. Surprisingly, bone from 10 -12-month-old cKO animals was indistinguishable from controls in size, density, and morphology except for a 25% increase in stiffness. cKO SOL exhibited increased expression of Pax7, Myog, Myod1, Notch, and Myh3 and 6-fold more centralized nuclei, characteristics of postnatal regenerating muscle, but only in type I myosin heavy chain-expressing cells. Increased expression of gene pathways mediating EGF receptor signaling, circadian exercise, striated muscle contraction, and lipid and carbohydrate oxidative metabolism were also observed in cKO SOL. This muscle phenotype was not observed in 3-month-old mice. Although Mbtps1 mRNA and protein expression was reduced in cKO bone osteocytes, no differences in Mbtps1 or cre recombinase expression were observed in cKO SOL, explaining this age-related phenotype. Understanding bone-muscle cross-talk may provide a fresh and novel approach to prevention and treatment of age-related muscle loss.Bone osteocytes are multifunctional endocrine cells. Osteoid osteocytes control bone mineralization by secretion of DMP1, phosphate-regulating endopeptidase homolog, X-linked (PHEX), and matrix extracellular phosphoglycoprotein (MEPE) (1), whereas mature osteocytes secrete sclerostin, an inhibitor of bone formation, and regulate phosphate homeostasis and, indirectly, bone mineralization via production of FGF23 (2). Osteocytes are the mechanosensory cells in bone and appear to regulate muscle function and myogenesis (3-5). Specifically, deletion of connexin43 in osteoblasts/osteocytes shows it is involved in postnatal bone-muscle cross-talk via an osteocalcin-mediated stimulation of muscle formation and function (5). Differentiated muscle cells, in return, secrete as yet unidentified factors that protect osteocytes against apoptosis and produce a factor that synergizes with fluid flow shear stress to increase prostaglandin E 2 production by osteocytes (6). Myogenesis in adult muscle occurs via activation of satellite (stem) cells during muscle regeneration after injury or exercise-induced damage (7,8). Distinct populations of satellite cells appear to be responsible for regeneration of fast twitch and slow twitch myofibers (9, 10), respectively. MBTPS1 (SKI-1, PCSK8, S1P) 3 is the eighth member of the proprotein convertase family of proteases (11). Localized to the cis/medial Golgi, MBTPS1 catalyzes the mandatory first cleavage of transmembrane-bound transcription factors like SREBP1 and cAMP-response element-binding protein; once released, their DNA binding domains are imported into the nucleus where they regulate transcription (12). Interestingly, MBTPS1 is required for the transcription of a number of bone matrix and mineralization-related genes including type XI collagen, Phex, Dmp1, fibronectin, and fibrillin ...

show abstract

“…Sarcalumenin is a Ca 2+ -binding protein located in the sarcoplasmic reticulum of striated muscle cells. Its physiological function has not been fully determined, but some studies have shown that sarcalumenin affected store-operated Ca 2+ entry and enhanced muscle fatigue resistance in sarcalumenin knockout mice (Zhao et al, 2005;Yoshida et al, 2005). The large difference in the expression of calcium-transporting ATPase and sarcalumenin in colonies C and M also suggested that colony C engaged in stronger muscular movements when infected with Varroa mites.…”

Section: Discussionmentioning

confidence: 99%

High-throughput sequencing identification of genes involved with Varroa destructor resistance in the eastern honeybee, Apis cerana

Yin

Liu

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Varroa destructor is the greatest threat to the honeybee Apis mellifera worldwide, while it rarely causes serious harm to its native host, the Eastern honeybee Apis cerana. The genetic mechanisms underlying the resistance of A. cerana to Varroa remain unclear. Thus, understanding the molecular mechanism of resistance to Varroa may provide useful insights for reducing this disease in other organisms. In this study, the transcriptomes of two A. cerana colonies were sequenced using the Illumina Solexa sequencing method. One colony was highly affected by mites, whereas the other colony displayed strong resistance to V. destructor. We determined differences in gene expression in the two colonies after challenging the colonies with V. destructor. After de novo transcriptome assembly, we obtained 91,172 unigenes for A. cerana and found that 288 differentially expressed genes varied by more than 15-fold. A total of 277 unigenes were present at higher levels in the non-affected colony. Genes involved in resistance to Varroa included unigenes related to skeletal muscle movement, olfactory sensitivity, and transcription factors. This suggests that hygienic behavior and grooming behavior

show abstract

Enhanced resistance to fatigue and altered calcium handling properties of sarcalumenin knockout mice

Cited by 73 publications

References 23 publications

Reduced expression of sarcalumenin and related Ca2+-regulatory proteins in aged rat skeletal muscle

Reduced expression of sarcalumenin and related Ca2+-regulatory proteins in aged rat skeletal muscle

Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age

High-throughput sequencing identification of genes involved with Varroa destructor resistance in the eastern honeybee, Apis cerana

Contact Info

Product

Resources

About