Enhanced saccharification of corn straw pretreated by alkali combining crude ligninolytic enzymes

Jiang, Li; Sun, Feng-Hui; Li, Xiangzhen; Yan, Zhiying; Yuan, Y.; Liu, Xiaofeng

doi:10.1002/jctb.3818

Cited by 23 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…50%) by SEC analysis 8% increment of glucose production [112] Alkali-extracted corn straw Trametes hirsuta Increment of porositiy and surface area in laccase-treated samples 2-fold increment in sugar production [113] Alkali-extracted straw from Brassica campestris Ganoderma lucidum…”

Section: Four Cycles Of Myceliophthora Thermophila Laccase -Alkaline mentioning

confidence: 99%

“…Properties such as porosity, surface area, and hydrophobicity can be altered, resulting in the reduction of unproductive binding of hydrolases. Li et al [113] observed an increment in the porosity and surface area of alkali-extracted corn straw after a treatment with Trametes hirsuta laccase, doubling the sugar production. The same effect was observed on alkali-extracted straw from Brassica campestris [114].…”

Section: Pretreated Materials Laccase Treatment Effects Observed Benefmentioning

confidence: 99%

See 1 more Smart Citation

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

View full text Add to dashboard Cite

Abstract:The continuous increase in the world energy and chemicals demand requires the development of sustainable alternatives to non-renewable sources of energy. Biomass facilities and biorefineries represent interesting options to gradually replace the present industry based on fossil fuels. Lignocellulose is the most promising feedstock to be used in biorefineries. From a sugar platform perspective, a wide range of fuels and chemicals can be obtained via microbial fermentation processes, being ethanol the most significant lignocellulose-derived fuel. Before fermentation, lignocellulose must be pretreated to overcome its inherent recalcitrant structure and obtain the fermentable sugars. Usually, harsh conditions are required for pretreatment of lignocellulose, producing biomass degradation and releasing different compounds that are inhibitors of the hydrolytic enzymes and fermenting microorganisms. Moreover, the lignin polymer that remains in pretreated materials also affects biomass conversion by limiting the enzymatic hydrolysis. The use of laccases has been considered as a very powerful tool for delignification and detoxification of pretreated lignocellulosic materials, boosting subsequent saccharification and fermentation processes. This review compiles the latest studies about the application of laccases as useful and environmentally friendly delignification and detoxification technology, highlighting the main challenges and possible ways to make possible the integration of these enzymes in future lignocellulose-based industries.

show abstract

Section: Four Cycles Of Myceliophthora Thermophila Laccase -Alkaline mentioning

confidence: 99%

Section: Pretreated Materials Laccase Treatment Effects Observed Benefmentioning

confidence: 99%

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Delignification assays can be performed at up to a liquid:solid ratio of 2:1, and it is customary to use solid charges in a ratio of (20:1 or less) . The enzymes added can vary between 4000 and 80 000 IU/g . Enzymatic delignification combined with other pretreatment technologies (as microbial delignification) to make it more effective.…”

Section: Organic Solventsmentioning

confidence: 99%

“…Ligninolytic enzymes as shown in Table are successfully used for a number of industrial applications, such as, organic compound manufacturing, polymer reformation, and removal of environmental toxins . The use of laccases to remove lignin was successfully applied to improve enzymatic hydrolysis . In the case of laccase enzyme, a suitable redox mediator must be present to stabilize in its redox states, and it should not inhibit the catalytic activity .…”

Section: Organic Solventsmentioning

confidence: 99%

“…116,119,120 The enzymes added can vary between 4000 and 80 000 IU/g. 120,121 Enzymatic delignification combined with other pretreatment technologies (as microbial delignification) to make it more effective. The pretreatment with laccases in combination with alkali pretreatment did not significantly improve the saccharification yields.…”

Section: Enzymatic Delignificationmentioning

confidence: 99%

See 1 more Smart Citation

Future prospects of delignification pretreatments for the lignocellulosic materials to produce second generation bioethanol

Baig

Turcotte

2019

Int J Energy Res

View full text Add to dashboard Cite

Summary Production of bioethanol is winning support from masses because it is a workable choice to solve the problems associated with the fluctuating prices of crude petroleum oil, climatic change, and reducing non‐renewable fuel reserves. First‐generation biofuels are produced directly from food crops. The biofuel (bioethanol, biodiesel) is ultimately derived from the starch, sugar, animal fats, and vegetable oil that these crops provide. It is important to note that the structure of the biofuel itself does not change between generations, but rather the source from which the fuel is derived changes. Corn, wheat, and sugar cane are the most commonly used first‐generation bioethanol feed stocks. Lignocellulosic materials are used as a feed stock for the production of second‐generation bioethanol. The major production steps are (1) delignification, (2) depolymerisation, and (3) fermentation. Agricultural residues are waste materials produced through the processing of agricultural crops. The main reason to use of these agricultural residues to produce bioethanol is to convert waste to value added products. The main challenges are the low yield of the cellulosic hydrolysis process due to the presence of lignin and hemicellulose with cellulose. Pretreatments of lignocellulosic materials to remove lignin and hemicellulose are the techniques used to enhance the hydrolysis. Present review article comprehensively discusses the different pretreatment methods of delignification for ethanol production. Published literature on pretreatments from 1982 to 2018 has been studied. Perspectives, gaps in studies, and recommendations are given to fully describe implementation of eight prominent pretreatments (milling, pyrolysis, organic solvents, steam explosion, hot water treatments, ozonolysis, enzymatic delignification, and genetic modification) for future research. The energy and environmental features of lignocellulosic materials are elaborated to show a sustainable aspect of second‐generation biofuel. It was felt necessary to discuss the concept of bio refinery to make biofuel production financially more attractive as well because the future prospects of second‐generation biofuel are promising.

show abstract

Exploring laccase and mediators behavior during saccharification and fermentation of steam‐exploded wheat straw for bioethanol production

Moreno

Ibarra²,

Alvira

et al. 2015

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Laccases represent a very powerful tool to improve biorefining processes from lignocellulosic feedstocks. These enzymes are being investigated not only for potential use as pretreatment agents in bioethanol production, mainly as a delignifying agent, but also as a biotechnological tool for removal of inhibitors (mainly phenols) of subsequent fermentation processes. RESULTS: In this work, the treatment of the water insoluble solids (WIS) fraction from steam-exploded wheat straw withPycnoporus cinnabarinus laccase and different laccase-mediator systems (LMS) did not decrease the lignin content, resulting in lower glucose recoveries during the subsequent saccharification. In combination with an alkaline extraction, the treatment with laccase/LMS produced no synergistic effect enhancing the delignification or saccharification of WIS. In contrast, laccase reduced the soluble phenols (95% of the total phenols identified) of the whole slurry from steam-exploded wheat straw, improving the yeast performance during the fermentation and enhancing the ethanol yields. CONCLUSIONS: The efficiency of P. cinnabarinus laccase with or without mediators as a delignifying agent on steam-exploded wheat straw for bioethanol production was not observed, whereas its detoxification ability was shown. Thus, new laccases or designing laccases with ability to delignify and detoxify simultaneously needs to be explored in order to produce major ethanol global yields.

show abstract

Enhanced saccharification of corn straw pretreated by alkali combining crude ligninolytic enzymes

Abstract: BACKGROUND: This work investigated the monokaryogenesis of dikaryon strains of Trametes hirsuta by protoplasts regeneration for extracellular ligninolytic enzyme production. Saccharification of corn straw was enhanced by alkali pretreatment combining crude ligninolytic enzymes.

Cited by 23 publications

References 58 publications

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

Future prospects of delignification pretreatments for the lignocellulosic materials to produce second generation bioethanol

Exploring laccase and mediators behavior during saccharification and fermentation of steam‐exploded wheat straw for bioethanol production

Contact Info

Product

Resources

About