Enhanced Saline Stress Resistance in Threonine and Methionine Overproducing Mutant Cell Line from Protoplast Culture of Rice (Oryza sativa L.)

Kwon, Taeho; Abe, Toshinori; Sasahara, Takeo

doi:10.1016/s0176-1617(11)81787-6

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…These results are in agreement with other in vitro studies for callus cultures of Brassica campestris (Paek et al, 1988) and shoots of 'Mr.S 2/5' peach rootstock (Dimassi-Theriou, 1998); however, K and Ca concentrations decreased (Table 2). Kingsbury et al (1984) and Kwon et al (1995) made similar observations for wheat (Triticum aestivum L.) and rice (Oryza sativa L.) This decrease suggests that there is a specific K · Na interaction in kiwifruit plantlets. Salinity may increase energy consumption, required for osmotic regulation and competition of transported ions.…”

Section: B and Nacl Effect On Shoot Mineral Compositionmentioning

confidence: 57%

Response to increasing rates of boron and NaCl on shoot proliferation and chemical composition of in vitro kiwifruit shoot cultures

Sotiropoulos

Dimassi

2004

Plant Cell Tiss Organ Cult

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The in vitro response of kiwifruit (Actinidia deliciosa) to increasing concentrations of boron (B) and NaCl in the culture medium was studied. Kiwifruit shoot cultures were grown in vitro for 12 weeks on an MS medium containing two B concentrations (0.1 and 2 mM) combined with five NaCl concentrations (0, 10, 20, 40 and 80 mM). Kiwifruit produced the longest shoots with 2 mM B when NaCl concentration was 0-20 mM. More shoots were produced with 2 mM B for all NaCl treatments. More shoots were produced with 2 mM B and 10 and 20 mM NaCl. High B concentrations in the culture medium significantly increased shoot proliferation. Explants exhibited a moderate chlorotic appearance with 40 mM NaCl and shoots died with 80 mM NaCl. With 2 mM B, the B concentration of explants was 5-9· greater for the various NaCl treatments compared to the control. Increasing the NaCl concentration from 10 to 80 mM, resulted in higher Na and Cl concentrations in explants for all B treatments, while K and Ca concentrations decreased. Phosphorus concentration in the explants was significantly increased by increasing the NaCl concentration reaching a maximum value at 80 mM NaCl for the two B concentrations.

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Section: B and Nacl Effect On Shoot Mineral Compositionmentioning

confidence: 57%

Response to increasing rates of boron and NaCl on shoot proliferation and chemical composition of in vitro kiwifruit shoot cultures

Sotiropoulos

Dimassi

2004

Plant Cell Tiss Organ Cult

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“…As KCl concentration in the culture medium increased, K concentration in tissues increased too. Potassium concentration of tissues of Nemared tended to be higher than those of GF 677 under the same KCl concentrations (Kwon et al 1995). Sodium concentration of tissues of both rootstocks decreased as KCl concentration of the media increased.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 82%

Responses of two Prunus rootstocks to KCl induced salinity in vitro

Sotiropoulos

Dimassi²,

Tsirakoglou³

et al. 2006

Biologia plant.

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The in vitro response of two Prunus rootstocks: GF 677 (Prunus persica × Prunus amygdalus), and Nemared (Prunus persica) to increasing concentrations of KCl of the culture medium was studied. Shoots were grown in vitro for 8 weeks on an Murashige and Skoog medium supplemented with 0, 5, 10, 15, 20, 40 or 80 mM KCl. By increasing KCl concentration from 0 to 40 mM, the number of shoots per explant was not significantly affected for both rootstocks. However, Nemared rootstock formed more shoots per explant than GF 677 under respective KCl concentrations of the medium. Inclusion of 80 mM KCl in the medium resulted in a reduction of growth of both rootstocks. Sodium, Fe, Mn, and Zn concentration in tissues of Nemared rootstock were significantly higher than the respective values of GF 677.Additional key words: cell proliferation, ion toxicity, micropropagation. ⎯⎯⎯⎯Salinity is one of the most important environmental factors limiting plant growth. Salt stress can adversely affect plant survival, biomass and plant height. Upon exposure to salt stress, plants exhibit a wide range of responses at the molecular, cellular and whole plant level (Hasegawa et al. 2000). These include morphological and developmental changes (e.g. life cycle, inhibition of shoot growth and enhancement of root growth), adjustment in ion transport (uptake, extrusion and sequestration of ions, decrease in uptake of K + , Ca 2+ and Mg 2+ ) and metabolic changes (osmoregulation) (Xiong and Zhu 2002). Nutrient imbalances were observed in salt-stressed plants (Grattan and Grieve 1999). Rootstocks affect the nutritional status of the scion in vitro (Sotiropoulos et al. 2005) and proper choice of rootstocks can ameliorate the detrimental effects of salinity (Layne 1987). The objective of the present research was to study the effect of KCl induced salinity on growth and nutritional status of two Prunus rootstocks cultured in vitro.The explants employed were shoots of two peach rootstocks: GF 677 (Prunus persica × Prunus amygdalus), and Nemared (Prunus persica). The shoots were about 25 mm in length preserved from previous in vitro cultures and maintained in the growth room. Each shoot was transferred and grown in a 15 × 100 mm glass test tube containing 5 cm 3 of the Murashige and Skoog (1962; MS) culture medium supplemented with 1 g m -3 benzyladenine (BA). Six KCl concentrations were used: 0, 5, 10, 15, 20, 40 and 80 mM. These KCl concentrations were added to the standard MS medium containing already 20.04 mM K + and 5.98 mM Cl -. Therefore K + concentration (mM) of the treatments were as follows: 20. 04, 25.04, 30.04, 35.04, 40.04, 60.04, 10.98, 15.98, 20.98, 25.98, 45.98, and 85.98. The pH of the media was adjusted to 5.8 before autoclaving at 121 °C for 15 min. The tubes were maintained in the growth room at 22 ± 1 °C and 16-h photoperiod per day (cool white fluorescent tubes, irradiance of 45 μmol m -2 s -1 , 400 -700 nm). After 8 weeks in culture, the number of shoots, length of shoots, and fresh and dry mass of explants were measured. For determi...

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“…1E,F). This may subsequently lead to a reduction of metabolically important ions such as K + (Kwon et al 1995). In contrast, K + content in contractile roots increased under salinity.…”

Section: Resultsmentioning

confidence: 99%

Contractile roots are the most sensitive organ in Crocus sativus to salt stress

Rajaei¹,

Niknam²,

Seyedi³

et al. 2009

Biologia plant.

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Crocus sativus corms were grown in Perlite and watered by half-strength modified Hoagland nutrient solution containing 0, 50, 100, 150, 200 mM NaCl. Growth parameters and contents of proteins, proline, polyphenols, minerals and saccharides were studied in fibrous roots, contractile roots, corms and leaves. All plants remained alive and did not display any sign of foliar damage even at 200 mM NaCl. However, the salinity decreased growth, relative water content and increased contents of proline and Na + in all organs. Total protein content was increased in corms and contractile roots but decreased in fibrous roots. Changes in protein pattern were also observed. Polyphenol content was increased by salinity in all organs except the leaves. As salinity increased, content of soluble saccharides decreased except in the contractile roots.

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Enhanced Saline Stress Resistance in Threonine and Methionine Overproducing Mutant Cell Line from Protoplast Culture of Rice (Oryza sativa L.)

Cited by 13 publications

References 19 publications

Response to increasing rates of boron and NaCl on shoot proliferation and chemical composition of in vitro kiwifruit shoot cultures

Response to increasing rates of boron and NaCl on shoot proliferation and chemical composition of in vitro kiwifruit shoot cultures

Responses of two Prunus rootstocks to KCl induced salinity in vitro

Contractile roots are the most sensitive organ in Crocus sativus to salt stress

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