Enhanced survival of ischemic skin flap by combined treatment with bone marrow-derived stem cells and low-level light irradiation

Abstract

The aim of this study is to examine the enhanced survival effect of ischemic skin flap by combined treatment with bone marrow-derived stem cells (BMSCs) and low-level light irradiation (LLLI). The neovasculogenic effect of BMSCs induced by LLLI was detected using a wound healing and tube formation assay. ICR mice were divided into four groups: control group, LLLI group, BMSCs group, and combine-treated group. The percentage of skin flap necrosis area was calculated on the seventh post-operative day. Specimens were harvested for histologic analyses. LLLI promoted BMSC migration and tube formation. The flap survival rate of combined treated group was significantly higher than that of the control group. Histologic results demonstrated a significant increase in neovascularization in the combined treatment group. This study demonstrates that combination treatment of BMSCs and LLLI could enhance the survival of ischemic skin flap in a mouse model.

Red (635 nm), Near-Infrared (808 nm) and Violet-Blue (405 nm) Photobiomodulation Potentiality on Human Osteoblasts and Mesenchymal Stromal Cells: A Morphological and Molecular In Vitro Study

“…The effectiveness of PBM to encourage proliferation and/or differentiation of osteogenic cells has been described in many in vitro studies [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. In addition, several pieces of in vivo research addressed the ability of PBM to accelerate the process of bone healing after oral and orthopedic surgery, by inducing an increase in bone density, neoformation, regeneration and mineralization [ 22 , 23 , 24 , 25 , 26 , 27 , 28 ] and some clinical trials are currently ongoing in different centers all over the world (Available online: ).…”

“…However, at present, univocal standardized guidelines for the use of PBM for osteoregenerative purposes are not available. This is mainly dependent on the variety of wavelengths, medical laser and LED types used with disparate energy output modes and setting parameters, which has produced many different treatment protocols with different and sometimes contradictory outcomes [ 13 , 20 , 21 , 26 , 29 , 30 ] hampering meaningful comparison of the results and demanding a skeptical look for the potential beneficial effects of this approach. Moreover, this scenario is complicated by the fact that a biphasic-dose cellular response to the same wavelength has been frequently observed with low levels of light that are more effective than the higher ones on stimulating cell functionality [ 31 ] In addition, the molecular mechanisms by which PBM induces different biological responses have not been fully clarified.…”

Polydopamine-tailored paclitaxel-loaded polymeric microspheres with adhered NIR-controllable gold nanoparticles for chemo-phototherapy of pancreatic cancer

“…Exposure of GNPs to NIR results in excitation of the surrounding electrons of GNPs. Hot electrons give their energy to the lattice, and through phonon–phonon scattering, heat is transferred to the tumor (Norouzi et al., 2018). This NIR-induced toxicity to pancreatic cancer cells was previously reported by Mocan et al., in which pretreatment with NIR laser-activated GNPs (2 W/cm 2 , 808 nm) enhanced the uptake of GNPs, and induced tumor toxicity in 1.4E7 pancreatic cancer cell line (Mocan et al., 2013).…”

Osteoblast differentiation of bone marrow stromal cells by femtosecond laser bone ablation

“…It is difficult to compare the efficacy of femtosecond laser ablation because of the wide variation in the methods and parameters employed in previous studies. In this in vitro study, BMSCs in bones were ablated with 1030-nm femtosecond lasers at a power of 10 W, which is much greater than that employed in other studies with low-level lasers [18,19]. Therefore, this study employed high-power femtosecond laser ablation to investigate its effect on BMSCs in greater detail.…”