Enhanced Survival of Neural Cells Embedded in Hydrogels Composed of Collagen and Laminin-Derived Cell Adhesive Peptide

Hiraoka, Masakatsu; Kato, Koichi; Nakaji-Hirabayashi, Tadashi; Iwata, Hiroo

doi:10.1021/bc9000068

Cited by 46 publications

(38 citation statements)

References 35 publications

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“…Several non-RGD peptides have been reported to promote NSC proliferation or differentiation, such as the laminin-derived PPFLMLLKGSTR [48] and IKVAV [7, 49] peptides. However, in previous work from our group, the IKVAV peptide failed to induce adhesion or proliferation of rat hippocampal NSCs when grafted to a polymer surface [25].…”

Section: Discussionmentioning

confidence: 99%

Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides

Ananthanarayanan¹,

Little²,

Schaffer³

et al. 2010

Biomaterials

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Peptide-functionalized materials show promise in controlling stem cell behavior by mimicking cellmatrix interactions. Supported lipid bilayers are an excellent platform for displaying peptides due to their ease of fabrication and low non-specific interactions with cells. In this paper, we report on the behavior of adult hippocampal neural stem cells (NSCs) on phospholipid bilayers functionalized with different RGD-containing peptides: either GGGNGEPRGDTYRAY ('bsp-RGD(15)') or GRGDSP. Fluid supported bilayers were prepared on glass surfaces by adsorption and fusion of small lipid vesicles incorporating synthetic peptide amphiphiles. NSCs adhered to bilayers with either GRGDSP or bsp-RGD(15) peptide. After 5 days in culture, NSCs formed neurosphere-like aggregates on GRGDSP bilayers, whereas on bsp-RGD(15) bilayers a large fraction of single adhered cells were observed, comparable to monolayer growth seen on laminin controls. NSCs retained their ability to differentiate into neurons and astrocytes on both peptide surfaces. This work illustrates the utility of supported bilayers in displaying peptide ligands and demonstrates that RGD peptides may be useful in synthetic culture systems for stem cells.

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Section: Discussionmentioning

confidence: 99%

Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides

Ananthanarayanan¹,

Little²,

Schaffer³

et al. 2010

Biomaterials

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“…[18,22] Table 1). In cell encapsulation systems, although there are currently growing attempts in mimicking the effects of physiological ECM by introducing various synthetic and natural ECM materials into synthetic polymers, [5,[23][24][25] no reported studies to date have addressed the impact of ECM components properties on synthetic hydrogel characteristics, especially in terms of permeability. The current research investigates biosynthetic hydrogels fabricated via incorporation of heparin and gelatin into a PVA network.…”

Section: Introductionmentioning

confidence: 99%

Structural and permeability characterization of biosynthetic PVA hydrogels designed for cell-based therapy

Nafea

Poole-Warren

Martens

2014

Journal of Biomaterials Science, Polymer Edition

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Incorporation of extracellular matrix (ECM) components to synthetic hydrogels has been shown to be the key for successful cell encapsulation devices, by providing a biofunctional microenvironment for the encapsulated cells. However, the influence of adding ECM components into synthetic hydrogels on the permeability as well as the physical and mechanical properties of the hydrogel has had little attention. Therefore, the aim of this study was to investigate the effect of incorporated ECM analogues on the permeability performance of permselective synthetic poly(vinyl alcohol) (PVA) hydrogels in addition to examining the physico-mechanical characteristics. PVA was functionalized with a systematically increased number of methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogel network. Heparin and gelatin were successfully incorporated into PVA network at low percentage (1%), and co-hydrogels were characterized for network properties and permeability to bovine serum albumin (BSA) and immunoglobulin G (IgG) proteins. Incorporation of these ECM analogues did not interfere with the base PVA network characteristics, as the controlled hydrogel mesh sizes, swelling and compressive modulii remained unchanged. While the permeation profiles of both BSA and IgG were not affected by the addition of heparin and gelatin as compared with pure PVA, increasing the FG/c from 7 to 20 significantly limited the diffusion of the larger IgG. Consequently, biosynthetic hydrogels composed of PVA with high FG/c and low percent ECM analogues show promise in their ability to be permselective for various biomedical applications.

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“…The cell‐binding domains were chosen to target major integrins expressed by skin‐derived primary human epidermal keratinocytes. For example, the alpha3beta1integrin expressed by keratinocytes, has been shown to bind the PPFLMLLKGSTR sequence the laminin‐5 alpha3 chain globular domain 3 (LG3) . Both the PHSRN and RGD peptides as well as full length fragments of the 9 th and 10 th domains of fibronectin (FN910) have also shown to increase cell‐matrix interaction of keratinocytes via the alpha5beta1 integrin .…”

Section: Introductionmentioning

confidence: 99%

Human Epidermal Keratinocyte Cell Response on Integrin‐Specific Artificial Extracellular Matrix Proteins

Tjin

Chua

Dong

et al. 2014

Macromolecular Bioscience

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Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering.

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Enhanced Survival of Neural Cells Embedded in Hydrogels Composed of Collagen and Laminin-Derived Cell Adhesive Peptide

Cited by 46 publications

References 35 publications

Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides

Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides

Structural and permeability characterization of biosynthetic PVA hydrogels designed for cell-based therapy

Human Epidermal Keratinocyte Cell Response on Integrin‐Specific Artificial Extracellular Matrix Proteins

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