Enhanced susceptibility to the GABA antagonist pentylenetetrazole during the latent period following a pilocarpine-induced status epilepticus in rats

Rattka, Marta; Brandt, Claudia; Bankstahl, Marion; Bröer, Sonja; Löscher, Wolfgang

doi:10.1016/j.neuropharm.2010.11.005

Cited by 60 publications

(62 citation statements)

References 55 publications

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“…Food (Altromin 1324 standard diet; Altromin, Lage, Germany) and water were freely available. Female animals were housed without males to keep them acyclic or asynchronous with respect to their estrous cycle 13, 14…”

Section: Methodsmentioning

confidence: 99%

The effects of carbamazepine in the intrahippocampal kainate model of temporal lobe epilepsy depend on seizure definition and mouse strain

Twele¹,

Töllner²,

Bankstahl³

et al. 2016

Epilepsia Open

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SummaryObjectiveMesial temporal lobe epilepsy (TLE) with hippocampal sclerosis is a predominant form of acquired epilepsy, characterized by recurrent simple and complex partial seizures that are often resistant to treatment. Mice developing spontaneous recurrent nonconvulsive and convulsive seizures after intrahippocampal injection of the excitotoxic glutamate agonist kainate are thought to represent a valuable model of mesial TLE. Epileptic electroencephalogram (EEG) activity recorded in this model from the kainate focus in the ipsilateral hippocampus is resistant to antiseizure drugs such as carbamazepine (CBZ). We compared the efficacy of CBZ in this model in two different mouse strains (FVB/N and NMRI). Furthermore, we evaluated whether changes in the definition of electrographic seizures affect the antiseizure efficacy of CBZ.MethodsAs in previous studies, two types of epileptic EEG activity were defined: high‐voltage sharp waves (HVSWs) and hippocampal paroxysmal discharges (HPDs). The characteristics of these paroxysmal EEG events in epileptic mice were compared with EEG criteria for nonconvulsive seizures in patients. For HVSWs, different spike frequencies, interevent intervals, and amplitudes were used as inclusion and exclusion criteria. In addition to CBZ, some experiments were performed with diazepam (DZP) and phenobarbital (PB).ResultsFemale epileptic FVB/N mice predominantly exhibited frequent HVSWs, but only infrequent HPDs or secondarily generalized convulsive seizures. Slight changes in HVSW definition determined whether they were resistant or responsive to CBZ. Male NMRI mice exhibited both HVSWs and HPDs. HVSWs were more resistant than HPDs to suppression by CBZ. Both types of epileptic EEG activity were rapidly suppressed by DZP and PB.SignificanceThe data demonstrate that focal electrographic seizures in the intrahippocampal kainate mouse model are less resistant than previously thought. Both mouse strain and the criteria chosen for definition of EEG seizures determine whether such seizures are drug‐resistant or ‐responsive.

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Section: Methodsmentioning

confidence: 99%

The effects of carbamazepine in the intrahippocampal kainate model of temporal lobe epilepsy depend on seizure definition and mouse strain

Twele¹,

Töllner²,

Bankstahl³

et al. 2016

Epilepsia Open

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“…For rating of seizure severity of spontaneous seizures, a modified Racine's scale [20] was used. As in a previous study in another post-SE model of TLE, seizures occurring during the first 1-3 days of recovery from SE were considered insult-associated symptomatic ("early") seizures [21].…”

Section: Monitoring Of Spontaneous Seizuresmentioning

confidence: 99%

“…In the 16 Harlan rats (group 3a), which were examined for alterations in behavior and PTZ seizure threshold in the three weeks following SE, seizure threshold was determined by timed intravenous infusion of the GABA A receptor antagonist PTZ as described in detail recently [21,25]. In each rat, the seizure threshold was determined at 1, 2, and 3 weeks after SE, thus allowing us to compare pre-and post-SE thresholds in the same rats.…”

Section: Seizure Threshold Determinationsmentioning

confidence: 99%

Refinement of a model of acquired epilepsy for identification and validation of biomarkers of epileptogenesis in rats

Brandt¹,

Rankovic²,

Töllner³

et al. 2016

Epilepsy & Behavior

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“…Indeed, data from several laboratories using acquired epilepsy models, including status epilepticus (SE) or TBI, suggest that this is true. [14][15][16] We recently demonstrated that rats with spontaneous seizures after TBI have the highest seizure susceptibility in the PTZ test. 11,17 The data obtained show that iSS can be predicted with a high sensitivity and specificity by using MRI.…”

mentioning

confidence: 99%

MRI Biomarkers for Post-Traumatic Epileptogenesis

Immonen

Kharatishvili

Gröhn

et al. 2013

Journal of Neurotrauma

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The present study tested a hypothesis that early identification of injury severity with quantitative magnetic resonance imaging (MRI) provides biomarkers for predicting increased seizure susceptibility and epileptogenesis after traumatic brain injury (TBI). TBI was induced by lateral fluid percussion injury (FPI) in adult rats. Quantitative T2, T1q, and diffusion were assessed with MRI at 9 days, 23 days, or 2 months post-TBI in the perilesional cortex, thalamus, and hippocampus. Seizure susceptibility was assessed at 12 months after TBI using the pentylenetetrazol seizure-susceptibility test. At 9 and 23 days post-TBI, a change in T1q of the perilesional cortex showed the greatest predictive value for increased seizure susceptibility at 12 months post-TBI [area under the curve (AUC), 0.929 and 0.952, respectively; p < 0.01]. At 2 months post-TBI, Dav in the thalamus was the best of the biomarkers analyzed (AUC, 0.988; p < 0.05). The highest predictive value of all biomarkers was achieved by combining the measurement of Dav in the perilesional cortex and the thalamus at 2 months post-TBI (AUC, 1.000; p < 0.01). Our results provide proof-of-concept evidence that clinically relevant MRI biomarkers predict increased seizure susceptibility after experimental TBI.

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Enhanced susceptibility to the GABA antagonist pentylenetetrazole during the latent period following a pilocarpine-induced status epilepticus in rats

Cited by 60 publications

References 55 publications

The effects of carbamazepine in the intrahippocampal kainate model of temporal lobe epilepsy depend on seizure definition and mouse strain

The effects of carbamazepine in the intrahippocampal kainate model of temporal lobe epilepsy depend on seizure definition and mouse strain

Refinement of a model of acquired epilepsy for identification and validation of biomarkers of epileptogenesis in rats

MRI Biomarkers for Post-Traumatic Epileptogenesis

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