Enhanced vacuole compartmentalization of cadmium in root cells contributes to glutathione-induced reduction of cadmium translocation from roots to shoots in pakchoi (Brassica chinensis L.)

Huang, Yifan; Chen, Jiahui; Zhang, Derui; Fang, Bo; YangJin, Tsering; Zou, Jianwen; Chen, Yahua; Su, Nana; Chen, Jin

doi:10.1016/j.ecoenv.2020.111616

Cited by 49 publications

(11 citation statements)

References 68 publications

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“…Cadmium is then loaded to the xylem and transported to the leaves by activation of ATP-dependent transporters HMA2 and HMA4 ( Verret et al, 2004 ; Wong and Cobbett, 2009 ; Sato-Nagasawa et al, 2012 ; Takahashi et al, 2012 ). In this sense, it was recently shown that cadmium increased the expression of IRT1, IRT2, HMA2, and HMA4 in Brassica chinensis inducing cadmium accumulation in roots ( Huang et al, 2021 ). Cadmium accumulation in plants involve the transporters HMA1, that transport cadmium with GSH to the vacuole ( Mendoza-Cózatl et al, 2011 ), HMA3 that transport free cadmium ions to the vacuole ( Sasaki et al, 2014 ), and ABCC1 and ABCC2 that transport cadmium with phytochelatins (PCs) to the vacuole ( Mendoza-Cózatl et al, 2011 ; Park et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa

2021

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In order to analyze the effect of cadmium in Ulva compressa (Chlorophyta), the alga was cultivated with 10, 25, and 50 μM of cadmium for 7 days, and the level of intracellular cadmium was determined. Intracellular cadmium showed an increase on day 1, no change until day 5, and an increase on day 7. Then, the alga was cultivated with 10 μM for 7 days, and the level of hydrogen peroxide, superoxide anions, and lipoperoxides; activities of antioxidant enzymes ascorbate peroxidase (AP), dehydroascorbate reductase (DHAR), and glutathione reductase (GR); the level of glutathione (GSH) and ascorbate (ASC); and the level of phytochelatins (PCs) and transcripts encoding metallothioneins (UcMTs) levels were determined. The level of hydrogen peroxide increased at 2 and 12 h, superoxide anions on day 1, and lipoperoxides on days 3 to 5. The activities of AP and GR were increased, but not the DHAR activity. The level of GSH increased on day 1, decreased on day 3, and increased again on day 5, whereas ASC slightly increased on days 3 and 7, and activities of enzymes involved in GSH and ASC synthesis were increased on days 3 to 7. The level of PC2 and PC4 decreased on day 3 but increased again on day 5. The level of transcripts encoding UcMT1 and UcMT2 increased on days 3 to 5, mainly that of UcMT2. Thus, cadmium accumulation induced an oxidative stress condition that was mitigated by the activation of antioxidant enzymes and synthesis of GSH and ASC. Then, the alga cultivated with inhibitors of calcium-dependent protein kinases (CDPKs), calmodulin-dependent protein kinases (CaMKs), calcineurin B-like protein kinases (CBLPKs), and MAPKs and 10 μM of cadmium for 5 days showed a decrease in intracellular cadmium and in the level of GSH and PCs, with the four inhibitors, and in the level of transcripts encoding UcMTs, with two inhibitors. Thus, CDPKs, CaMK, CBLPKS, and MAPKs are involved in cadmium accumulation and GSH and PC synthesis, and GSH and PCs and/or UcMTs may participate in cadmium accumulation.

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Section: Introductionmentioning

confidence: 99%

Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa

2021

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“…It has been reported that the Cd subcellular reallocation determines Cd resistance in plants [15, 19, 41]. CW fixation and vacuole compartmentalization both play pivotal roles in enhancing plant Cd resistance [9, 16, 48]. 0.3% IDS application had no remarkable effect on Cd sequestration into vacuoles, but promoted Cd fixation in CWs, thereby decreasing Cd stress in organelles (Figure 3).…”

Section: Discussionmentioning

confidence: 97%

Iminodisuccinic Acid Relieved Cadmium Stress in Rapeseed Leaf by Affecting Cadmium Distribution and Cadmium Chelation with Pectin

Tian

Zhu

Song

et al. 2022

Adsorption Science & Technology

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Rapeseed (Brassica napus L.) is a nutritious vegetable, while cadmium (Cd) pollution threatens the growth, productivity, and food security of rapeseed. By studying the effects of iminodisuccinic acid (IDS), an easily biodegradable and environmental friendly chelating agent, on Cd distribution at the organ and cellular level, we found IDS promoted dry matter accumulation of rapeseed and increased the contents of photosynthetic pigment in leaves. Inhibited root-shoot Cd transport resulted in higher activity of antioxidant enzymes and decreased hydrogen peroxide (H2O2) and malondialdehyde (MDA) accumulation in leaves, which indicated that IDS contributed to alleviating Cd-caused oxidative damage in leaf cells. Additionally, IDS increased Cd subcellular distribution in cell wall (CW), especially in covalently bound pectin (CSP), and relieved Cd toxicity in organelle of leaves. IDS also enhanced demethylation of CSP. The Cd content in CSP, demethylation degree, and pectin methylesterase activity of CSP increased by 37.95%, 13.34%, and 13.16%, respectively, while IDS did not change the contents of different CW components. The improved Cd fixation in leaf CW was mainly attributed to enhance demethylation of covalently bound pectin (CSP) and Cd chelation with CSP.

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“…Figures and tables were generated using Excel 2016 and SigmaPlot 12.5 software. The translocation factor (TF), an indicator of the ability of plants to translocate As or Cd from roots to shoots, was calculated as the ratio of As or Cd concentration in shoots to roots ( He et al., 2015 ; Huang et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The subcellular distribution of As and Cd significantly affects the concentration of free As and Cd ions in plant cells, modulating As and Cd translocation and toxicity to plants ( Srivastava et al., 2016 ; Cao et al., 2018 ; Huang et al., 2021 ). In rice, S supply alters the Cd subcellular distribution by regulating OsHMA3 (OsHMA3, a transporter for Cd sequestration into root vacuoles) expression ( Cao et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Sulfur reduces the root-to-shoot translocation of arsenic and cadmium by regulating their vacuolar sequestration in wheat (Triticum aestivum L.)

et al. 2022

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Accumulation of arsenic (As) and cadmium (Cd) in wheat grain is a serious threat to human health. Sulfur (S) can simultaneously decrease wheat grain As and Cd concentrations by decreasing their translocation in wheat; however, the mechanisms are unclear. We conducted hydroponic experiments to explore the mechanisms by which S modulates As and Cd translocation and their toxicity in wheat. Wheat seedlings were grown in deficient sulfate (2.5 µM) or sufficient sulfate (1.0 mM) nutrient solutions for 6 days and then exposed to zero (control), low As+Cd (1 µM As plus 0.5 µM Cd), or high As+Cd (50 µM As plus 30 µM Cd) for another 6 days. Compared with the control, plant growth was not affected by low As+Cd, but was significantly inhibited by high As+Cd. In the low As+Cd treatment, S supply had no significant effect on plant growth or root-to-shoot As and Cd translocation. In the high As+Cd treatment, sufficient S supply significantly alleviated As and Cd toxicity and their translocation by increasing phytochelatin (PC) synthesis and the subsequent vacuolar sequestration of As and Cd in roots, compared with deficient S supply. The use of L-buthionine sulfoximine (a specific inhibitor of γ-glutamylcysteine synthetase) confirmed that the alleviation of As and Cd translocation and toxicity in wheat by S is mediated by increased PC production. Also, TaHMA3 gene expression in wheat root was not affected by the As+Cd and S treatments, but the expression of TaABCC1 was upregulated by the high As+Cd treatment and further increased by sufficient S supply and high As+Cd treatment. These results indicate that S-induced As and Cd subcellular changes affect As and Cd translocation mainly by regulating thiol metabolism and ABCC1 expression in wheat under As and Cd stress.

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Enhanced vacuole compartmentalization of cadmium in root cells contributes to glutathione-induced reduction of cadmium translocation from roots to shoots in pakchoi (Brassica chinensis L.)

Cited by 49 publications

References 68 publications

Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa

Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa

Iminodisuccinic Acid Relieved Cadmium Stress in Rapeseed Leaf by Affecting Cadmium Distribution and Cadmium Chelation with Pectin

Sulfur reduces the root-to-shoot translocation of arsenic and cadmium by regulating their vacuolar sequestration in wheat (Triticum aestivum L.)

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