Enhanced vascular effects of the Ca²⁺channel agonist Bay K 8644 in pregnant rabbits

Abstract

Hemodynamic studies were performed to determine if blunting of vascular pressor responsiveness to vasoconstrictors during pregnancy may be due to impaired L-type voltage-dependent calcium channels (L-VDCC). Bay K 8644 (BAY), an L-VDCC agonist, was infused in pregnant and nonpregnant anesthetized rabbits (10, 20, 40, and 60 μg/kg) and pregnant and nonpregnant conscious, chronically instrumented (conscious) rabbits (10, 25, and 50 μg/kg). BAY infusions resulted in greater elevation of mean arterial pressure in both anesthetized pregnant ( n = 6) vs. nonpregnant ( n = 6) ( P < 0.05) and conscious pregnant ( n = 10) vs. nonpregnant ( n = 10) rabbits ( P < 0.05). Fractional increase over baseline of total peripheral resistance index was greater in pregnant (36 ± 5 to 78 ± 14%) vs. nonpregnant rabbits (14 ± 4 to 52 ± 6%) ( P< 0.02). Cardiac output index did not differ. There was a single high-affinity L-VDCC antagonist aortic binding site with similar number and affinity in pregnant ( n = 7) and nonpregnant ( n = 7) rabbits. In conclusion, stimulation of L-VDCC induces greater pressor responses in pregnant rabbits with heightened peripheral vasoconstriction. This does not appear to be due to a change in L-VDCC receptor parameters.