Enhanced yields of measles virus from cultured cells

Key Parameters of Measles Virus Production for Oncolytic Virotherapy

“…MV production in the suspension cell line U937 resulted in a titer of 6×10 6 pfu mL −1 (Brandriss et al, 1982). A comparison of Vero, BGMK, CV-1, Hep-2, AV 3 , BHK-21, PK-15 and MDCK cells infected with the Edmonston strain revealed that Vero and BGMK cells produced the highest virus yields (Scott and Choppin, 1982). The yield with Vero cells was slightly higher with a maximum value of 2.8×10 8 pfu mL −1 .…”

“…However, besides the parameters cell types and culture mode, the medium supplements have also been varied during the three culture set-ups (Baczko and Lazzarini, 1979). Thus, no absolute comparison is feasible, since the impact of medium composition on MV production yields has already been documented, as well (Romano and Scarlata, 1973;Scott and Choppin, 1982). Taken together, these data may be helpful for choosing an optimal MV producer cell line, but have to be interpreted with respect to the composition of the medium.…”

“…Other cell lines had reduced virus production, with 7-28,000 times lower titres. MDCK cells had the lowest production rate with just 1×10 4 pfu mL −1 (Scott and Choppin, 1982).…”

“…The maximum virus yield obtained with the Edmonston strain has been determined by different groups, while various cell lines were infected with this MV strain (Black, 1959;Kohn and Yassky, 1962;Nakai et al, 1969;Romano and Scarlata, 1973;Baczko and Lazzarini, 1979;Brandriss et al, 1982;Scott and Choppin, 1982;Mendonca et al, 1994; (Table 2).…”

“…These are the Hallé strain (Dubois-Dalcq and Reese, 1975;Scott and Choppin, 1982), the TYCSA strain (a variant of the Toyoshima strain) (Shishido et al, 1967), the Leningrad-16 strain (Boriskin et al, 1988;Sidorenko et al, 1989) and the AIK-C strain (Trabelsi et al, 2010) (Table 2).…”