Enhanced β2-adrenergic receptor (β2AR) signaling by adeno-associated viral (AAV)-mediated gene transfer

Abstract

Background: β 2 -Adrenergic receptors (β 2 AR) play important regulatory roles in a variety of cells and organ systems and are important therapeutic targets in the treatment of airway and cardiovascular disease. Prolonged use of β-agonists results in tolerance secondary to receptor down-regulation resulting in reduced therapeutic efficiency. The purpose of this work is to evaluate the signaling capabilities of the β 2 AR expressed by a recombinant adeno-associated viral (AAV) vector that also included an enhanced green fluorescent protein (EGFP) gene (AAV-β 2 AR/EGFP).

Influence of Lipid Composition on the Structural Stability of G-Protein Coupled Receptor

“…2) β 2 AR plays an important regulatory role in a variety of cells and organs and is an important therapeutic target in the treatment of airway and cardiovascular disease. 3) Like other GPCRs, the conformation of β 2 AR is known to vary between the inactive and the active states. The conformational change induced by the binding of extracellular ligands to β 2 AR results in the Gprotein-mediated cell signaling.…”