Enhancement by Gangliosides of the Binding of Serotonin to Serotonin Binding Protein

Tamir, Hadassah; Brunner, William; Casper, Diana; Rapport, Maurice M.

doi:10.1111/j.1471-4159.1980.tb11266.x

Cited by 55 publications

(14 citation statements)

References 26 publications

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“…Similar requirements with regard to temperature, ATP, Mg2+, and sensitivity to added reserpine were observed for 5-HT uptake by rat brain synaptic vesicles (Kaladhar, 1977;Halaris and Demet, 1978). Tamir et al (1980) successfully demonstrated the presence of SBP in synaptic vesicles. In an earlier study (Tamir et al, 1976) they were able to demonstrate a specific role for Fe2+ in enhancing 5-HT binding to SBP by several-fold.…”

Section: Maintenance Of High Monoamine Concentrationsmentioning

confidence: 96%

“…Although nonhaem iron in the brain is shown to be associated with certain enzymes and compounds purified serotonin binding protein (SBP) (Tamir et al, 1976). They have demonstrated subsequently the presence of SBP in synaptic vesicles, thus suggesting a role for iron in 5-HT storage in the brain (Tamir and Gershen, 1979;Tamir et al, 1980). We have therefore undertaken studies to investigate the effects of moderate to severe iron deficiency on 5-HT uptake into rat brain synaptic vesicles in vifro, the results of which are reported in the present communication.…”

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confidence: 99%

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Effects of Iron Deficiency on Serotonin Uptake In Vitro by Rat Brain Synaptic Vesicles

Kaladhar

Rao

1982

Journal of Neurochemistry

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The effects of moderate and severe degrees of iron deficiency on brain and liver nonhaem iron levels and 5-hydroxytryptamine (serotonin; 5-HT) uptake by synaptic vesicles in vitro were investigated in experimental rats. Data obtained suggested that in both moderate and severe forms of iron deficiency, 5-HT uptake by brain synaptic vesicles is decreased and is accompanied by a reduction in brain and liver nonhaem iron levels. On repletion with iron for 4 weeks, the deficient group of rats showed a normalisation of 5-HT uptake by synaptic vesicles and liver nonhaem iron content, whereas the brain nonhaem iron concentration still showed a significant deficit. The data thus suggest that changes in the uptake of 5-HT by brain synaptic vesicles that accompany iron depletion and repletion are more rapid than changes in the total nonhaem iron concentration in the brain. The observation that 5-HT uptake by brain synaptic vesicles is decreased in iron deficiency suggests a probable role for iron in 5-HT storage in rat brain.

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Section: Maintenance Of High Monoamine Concentrationsmentioning

confidence: 96%

mentioning

confidence: 99%

Effects of Iron Deficiency on Serotonin Uptake In Vitro by Rat Brain Synaptic Vesicles

Kaladhar

Rao

1982

Journal of Neurochemistry

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“…Gangliosides seem to have an important role in the functioning of the 5-HT system (Tamir et al, 1980;Agnati et al, 1983;Adlersberg et al, 1987).…”

Section: Neurotrophic Factors Andmentioning

confidence: 97%

The treatment of Alzheimer's disease

Allen

Burns

1995

J Psychopharmacol

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“…Gangliosides are sialic-acid-containing glycolipids present in cell membranes, where they have been implicated as receptors for cholera toxin [10], interferon [3], hormones [27,48], viruses [2,32] and growth factors [4,5]. Addition of gangliosides to cultures of human lymphocytes was shown to inhibit their responses to lectin and antigens [13,31,54] Offprint requests to: P. Hersey, Room 443, Immunology and Oncology Unit, David Maddison Clinical Sciences Building, Royal Newcastle Hospital, Newcastle, N.S.W.…”

Section: Introductionmentioning

confidence: 99%

Potentiation of interleukin-2 production and its binding by monoclonal antibodies to the gangliosides GD3 and GD2

Schibeci

Hersey

Cheresh

1989

Cancer Immunol Immunother

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Previous studies have shown that monoclonal antibodies (mAbs) against certain gangliosides, which induced remissions in patients with melanoma, also potentiated the response of lymphocytes to a variety of stimuli, including lectins, interleukin-2 (IL-2) and antigens. The present studies have investigated the mechanism of these effects on lymphocytes. Although the mAbs potentiated phytohemagglutinin(PHA)-induced IL-2 production at high concentrations of mAbs and of PHA, this did not appear to explain their potentiation of the proliferative responses of lymphocytes. Hence, although IL-2 production was minimal or absent from the CD8+ subset the latter showed the highest degree of augmentation. Furthermore, addition of IL-2 to PHA-stimulated cultures did not produce similar augmentation of mitogenic responses to that produced by the mAb to GD3 or GD2. The augmented and normal mitogenic responses were, however, dependent on IL-2, as shown by their inhibition with mAbs against IL-2. The antiganglioside mAbs did not have significant effects on IL-2 receptor expression measured by mAbs to Tac. However, the mAbs appeared to increase the affinity of binding of radiolabelled IL-2 to IL-2 receptor and increased internalization of the latter. These results suggest that the effects of the mAbs on IL-2 production may be distinguished from their effects on the proliferative responses of T cells and that the latter were associated with changes in affinity and internalization of 125I-IL-2. Whether the latter is a direct cause of the increased proliferative response remains unknown. The ability of mAbs to GD2 and GD3 to increase IL-2 production and to "enhance" IL-2-dependent proliferative responses suggests the may have valuable clinical roles as immunopotentiating agents.

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Enhancement by Gangliosides of the Binding of Serotonin to Serotonin Binding Protein

Cited by 55 publications

References 26 publications

Effects of Iron Deficiency on Serotonin Uptake In Vitro by Rat Brain Synaptic Vesicles

Effects of Iron Deficiency on Serotonin Uptake In Vitro by Rat Brain Synaptic Vesicles

The treatment of Alzheimer's disease

Potentiation of interleukin-2 production and its binding by monoclonal antibodies to the gangliosides GD3 and GD2

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