Enhancement in Iron Absorption on Intake of Chemometrically Optimized Ratio of Probiotic Strain Lactobacillus plantarum 299v with Iron Supplement Pearl Millet

Adiki, Shanta Kumari; Perla, Chandra Kiran; Saha, Goutam; Katakam, Prakash; Theendra, Vinay Kumar

doi:10.1007/s12011-018-1541-5

Cited by 13 publications

(12 citation statements)

References 7 publications

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“…It has previously been shown that the intake of Lp299v improves intestinal iron absorption in meal studies [31][32][33]. In addition, the intake of Lp299v has been shown to increase the hemoglobin and hematocrit values in anemic rats [41]. The beneficial effect of Lp299v on iron absorption has also been recently examined in a systematic review and meta-analysis [42].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Lactobacillus plantarum 299v on Iron Status and Physical Performance in Female Iron-Deficient Athletes: A Randomized Controlled Trial

Axling

Önning

Combs³

et al. 2020

Nutrients

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Iron is an essential micronutrient for oxygen transport and mitochondrial metabolism and is critical for physical performance. Compromised iron stores are more commonly found among athletes, and females are especially at risk. Iron deficiency is generally treated using oral iron supplements. However, only a small proportion of ingested iron is absorbed, necessitating higher intakes, which may result in adverse side effects, reduced compliance, and inefficient repletion of iron stores. The probiotic strain Lactobacillus plantarum 299v (Lp299v) significantly increases intestinal iron absorption in meal studies. The present study was conducted to explore the effects of 20 mg of iron with or without Lp299v on iron status, mood state, and physical performance. Fifty-three healthy non-anemic female athletes with low iron stores (ferritin < 30 μg/L) were randomized, and 39 completed the study. Intake of Lp299v with iron for four weeks increased ferritin levels more than iron alone (13.6 vs. 8.2 µg/L), but the difference between the groups was not significant (p = 0.056). The mean reticulocyte hemoglobin content increased after intake of Lp299v compared to control (1.5 vs. 0.82 pg) after 12 weeks, but the difference between the group was not significant (p = 0.083). The Profile of Mood States (POMS) questionnaire showed increased vigor with Lp299v vs. iron alone after 12 weeks (3.5 vs. 0.1, p = 0.015). No conclusive effects on physical performance were observed. In conclusion, Lp299v, together with 20 mg of iron, could result in a more substantial and rapid improvement in iron status and improved vigor compared to 20 mg of iron alone. A larger clinical trial is needed to further explore these findings as well as the impact of Lp299v on physical performance.

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Section: Discussionmentioning

confidence: 99%

The Effect of Lactobacillus plantarum 299v on Iron Status and Physical Performance in Female Iron-Deficient Athletes: A Randomized Controlled Trial

Axling

Önning

Combs³

et al. 2020

Nutrients

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“…In an in vitro study, Sandberg et al has shown that a probiotic Lactobacillus plantarum 299v (Lp299v) effect on Fe absorption is mediated through the Fe 3+ /DCYTB axis-ferric reductase DCYTB is increased in the presence of Lactobacillus plantarum 299v (Lp299v) [45]. In an animal study, Adiki et al documented that probiotic Lactobacillus plantarum 299v (Lp299v) increases Fe absorption from the diet, and this effect was independent from probiotic dose [46]. In human studies, Hoppe et al [43] have shown that intake of Lactobacillus plantarum 299v (Lp299v) ameliorates iron absorption from a fruit drink.…”

Section: Discussionmentioning

confidence: 99%

“…However, EFSA did not approve the application stating that there is no sufficient evidence for the claim and that no plausible mechanism of Lactobacillus plantarum 299v was pointed [44]. EFSA refusal initiated scientific teams to perform further studies, not only human, but also in vitro [45] and animal [46], showing a beneficial effect of Lactobacillus plantarum 299v on Fe absorption. In our study, we have implemented multistrain probiotic consisted of nine bacterial strains: Bifidobacterium bifidum W23, B. lactis W51, B. lactis W52, Lactobacillus acidophilus W37, L. brevis W63, L. casei W56, L. salivarius W24, Lactococcus lactis W19, and Lc.…”

Section: Introductionmentioning

confidence: 99%

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

et al. 2020

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The influence of probiotic supplementation on iron metabolism remains poorly investigated. However, a range of studies, especially on Lactobacillus plantarum 299v (Lp229v), have indicated a possible positive impact of probiotics on iron absorption. The aim of the study was to determine the effect of multistrain probiotic supply on iron balance. Thirty Wistar rats were randomized into three groups: placebo (KK group), and multistrain probiotic per os in a daily dose of 2.5 × 109 colony forming units (CFU) (PA group) or 1 × 1010 CFU (PB group). Multistrain probiotic consisted of nine bacterial strains: Bifidobacterium bifidum W23, B. lactis W51, B. lactis W52, Lactobacillus acidophilus W37, L. brevis W63, L. casei W56, L. salivarius W24, Lactococcus lactis W19, and Lc. lactis W58, in equal proportions. After six weeks, blood and organ samples were collected. No differences were found between the three groups in terms of serum concentrations of hepcidin (HEPC), lactoferrin (LTF), homocysteine (HCY), ferritin (Ft), or erythroferrone (ErFe), or in liver content of divalent metal transporter 1 (DMT1), transferrin receptors 1 and 2 (TfR), or ZRT/IRT-like protein 14 (ZIP14) proteins. In the overall sample, positive correlations were noted between the serum concentrations of hepcidin and lactoferrin, and hepcidin and ferritin; serum concentration of hepcidin and DMT1 and TfR1 in the liver; and serum concentration of erythroferrone and TfR2 in the liver. The correlations of serum hepcidin and erythroferrone with liver DMT1 and TfR represent significant mechanisms of Fe homeostasis. Our study has shown that multistrain probiotic supplementation used in the experiment did not disrupt the biochemical and hepatic regulatory processes of Fe balance and did not demonstrate significant influence on selected parameters of Fe metabolism.

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“…Increased iron uptake independent of food matrix L. plantarum 299v has been shown to increase iron absorption in several clinical studies in women of childbearing age (Bering et al, 2006;Hoppe et al, 2015Hoppe et al, , 2017 and improve iron status in clinical (Axling et al, 2020, in press;Korcok et al, 2018) and animal (Adiki et al, 2019) studies. The studies by Bering et al (2006Bering et al ( , 2007 and Hoppe et al (2015Hoppe et al ( , 2017 all made use of a double-isotope cross-over design method.…”

Section: Iron Absorptionmentioning

confidence: 99%

Lactiplantibacillus plantarum 299v (LP299V®): three decades of research

Nordström

Teixeira

Montelius

et al. 2021

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This review aims to provide a comprehensive overview of the in vitro, animal, and clinical studies with the bacterial strain Lactiplantibacillus plantarum 299v (L. plantarum 299v; formerly named Lactobacillus plantarum 299v) published up until June 30, 2020. L. plantarum 299v is the most documented L. plantarum strain in the world, described in over 170 scientific publications out of which more than 60 are human clinical studies. The genome sequence of L. plantarum 299v has been determined and is available in the public domain (GenBank Accession number: NZ_LEAV01000004). The probiotic strain L. plantarum 299v was isolated from healthy human intestinal mucosa three decades ago by scientists at Lund University, Sweden. Thirty years later, a wealth of data coming from in vitro, animal, and clinical studies exist, showing benefits primarily for gastrointestinal health, such as reduced flatulence and abdominal pain in patients with irritable bowel syndrome (IBS). Moreover, several clinical studies have shown positive effects of L. plantarum 299v on iron absorption and more recently also on iron status. L. plantarum 299v is safe for human consumption and does not confer antibiotic resistance. It survives the harsh conditions of the human gastrointestinal tract, adheres to mannose residues on the intestinal epithelial cells and has in some cases been re-isolated more than ten days after administration ceased. Besides studying health benefits, research groups around the globe have investigated L. plantarum 299v in a range of applications and processes. L. plantarum 299v is used in many different food applications as well as in various dietary supplements. In a freeze-dried format, L. plantarum 299v is robust and stable at room temperature, enabling long shelf-lives of consumer healthcare products such as capsules, tablets, or powder sachets. The strain is patent protected for a wide range of indications and applications worldwide as well as trademarked as LP299V®.

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Enhancement in Iron Absorption on Intake of Chemometrically Optimized Ratio of Probiotic Strain Lactobacillus plantarum 299v with Iron Supplement Pearl Millet

Cited by 13 publications

References 7 publications

The Effect of Lactobacillus plantarum 299v on Iron Status and Physical Performance in Female Iron-Deficient Athletes: A Randomized Controlled Trial

The Effect of Lactobacillus plantarum 299v on Iron Status and Physical Performance in Female Iron-Deficient Athletes: A Randomized Controlled Trial

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

Lactiplantibacillus plantarum 299v (LP299V®): three decades of research

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