Enhancement of 2,3‐Butanediol Production by <i>Klebsiella oxytoca</i> PTCC 1402

Anvari, Masumeh; Motlagh, Mohammad Reza Safari

doi:10.1155/2011/636170

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…In 2, 3‐BDO fermentation, acetate has been identified as not only an inhibitor but also a substrate to K. oxytoca . Acetate might be an inducer for 2, 3‐BDO pathway genes, and in its ionized form induces acetolactate synthase formation and thereby enhances the catalysis of pyruvate to 2, 3‐BDO . This could explain why 2, 3‐BDO yield in medium with acetic acid addition is higher.…”

Section: Discussionmentioning

confidence: 99%

“…Acetate might be an inducer for 2, 3-BDO pathway genes, and in its ionized form induces acetolactate synthase formation and thereby enhances the catalysis of pyruvate to 2, 3-BDO. 20 -22 This could explain why 2, 3-BDO yield in medium with acetic acid addition is higher. A decrease in 2, 3-BDO yield was observed when acetic acid concentration reached a certain value, which is decided by the amounts of furfural, HMF and acetic acid in medium.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of acetic acid, furfural and 5‐hydroxymethylfurfural affecting 2, 3‐butanediol production using Klebsiella oxytoca

Cheng

Wang

et al. 2013

J of Chemical Tech & Biotech

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BACKGROUND The effect of the combination of acetic acid, furfural and 5‐hydroxymethylfurfural on 2, 3‐butanediol fermentation of Klebsiella oxytoca was studied by statistical analysis. The compounds were selected taking into account their concentration in the hydrolysate from the acid pretreatment of biomass and their toxicity. A central composite design was used to investigate the influences of these compounds on 2, 3‐butanediol yield and biomass formation. RESULTS Two models were developed to describe the effect of the combination of acetic acid, furfural and 5‐hydroxymethylfurfural on biomass and product yield in 2, 3‐butanediol fermentation. The proposed model is suited to fit experimental data and to simulate cell growth and 2, 3‐butanediol yield, which was confirmed by 2, 3‐butanediol production using real hydrolysate as substrate. CONCLUSION Biomass yield was affected mainly by the ‘main effects’ of the tested compounds and no interaction relationship among acetic acid, furfural and HMF was found. 2, 3‐butanediol yield was more sensitive to acetic acid concentration. Selective removal of furfural in hydrolysate would contribute to the simultaneous improvement of 2, 3‐butanediol yield and cell growth. © 2013 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Analysis of acetic acid, furfural and 5‐hydroxymethylfurfural affecting 2, 3‐butanediol production using Klebsiella oxytoca

Cheng

Wang

et al. 2013

J of Chemical Tech & Biotech

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“…Other authors have previously reported that yeast extract, ammonium acetate, ammonium sulfate, glycerol, and tryptone, as well as temperature and inoculum size influence microbial 2,3-BD production [16][17][18][19][20]. Some of these studies concluded that using high concentrations of expensive yeast extract (up 60 g/L) was crucial for optimal 2,3-BD production.…”

Section: Experimental Design and Data Analysismentioning

confidence: 99%

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

et al. 2017

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While chiral 2,3-Butanediol (2,3-BD) is currently receiving remarkable attention because of its numerous industrial applications in the synthetic rubber, bioplastics, cosmetics, and flavor industries, 2,3-BD-mediated feedback inhibition of Paenibacillus polymyxa DSM 365 limits the accumulation of higher concentrations of 2,3-BD in the bioreactor during fermentation. The Box-Behnken design, Plackett-Burman design (PBD), and response surface methodology were employed to evaluate the impacts of seven factors including tryptone, yeast extract, ammonium acetate, ammonium sulfate, glycerol concentrations, temperature, and inoculum size on 2,3-butanediol (2,3-BD) production by Paenibacillus polymyxa DSM 365. Results showed that three factors; tryptone, temperature, and inoculum size significantly influence 2,3-BD production (p < 0.05) by P. polymyxa. The optimal levels of tryptone, inoculum size, and temperature as determined by the Box-Behnken design and response surface methodology were 3.5 g/L, 9.5%, and 35 • C, respectively. The optimized process was validated in batch and fed-batch fermentations in a 5-L Bioflo 3000 Bioreactor, and 51.10 and 68.54 g/L 2,3-BD were obtained, respectively. Interestingly, the production of exopolysaccharides (EPS), an undesirable co-product, was reduced by 19% when compared to the control. These results underscore an interplay between medium components and fermentation conditions, leading to increased 2,3-BD production and decreased EPS production by P. polymyxa. Collectively, our findings demonstrate both increased 2,3-BD titer, a fundamental prerequisite to the potential commercialization of fermentative 2,3-BD production using renewable feedstocks, and reduced flux of carbons towards undesirable EPS production.

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“…BP:B oron phosphate was synthesized by using an organic precursor,t ri-iso-propylborate (B(iPrO) 3 ). B(iPrO) 3 was mixed with orthophosphoric acid (85 %). The B/P molar ratio was equal to 1.…”

Section: Catalyst Preparationmentioning

confidence: 99%

“…Its ester derivatives are precursors of polyurethane and can be applied in cosmetic, pharmaceutical, and other industries. [1,3] But the most important applications are connected with BDO dehydration into butan-2-one (methyl ethyl ketone,M EK), 3-buten-2-ol( 3B2OL), and 1,3-butadiene (BD). [1b, 4-6] MEK is aw idely used organic solvent as well as ap recursor for MEK peroxide.…”

Section: Introductionmentioning

confidence: 99%

Dehydration of 2,3-butanediol over zeolite catalysts

2016

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opment of the ethylene industry are such that ethylene producers are switchingt om ore efficient ethylene production technologies. This leads to al ack of butadiene on the market and therefore requires the development of alternative technologies leadingt ob utadiene.T herefore, the synthesis of MEK, BD, and 3B2OL from BDO appears to be achallenging goal.Synthesis of MEK from BDO has been reportedo ver strong solid catalysts such as H-Nafion, [10] heteropoly acids, [11] aluminosilicates, [12] and zeolites. [1f, 13, 14] Bucsi et al. [10] reported high conversions of BDO into MEK over H-Nafion catalysts at temperatures as low as 150 8C; the acetalization of MEK with BDO being the main side reaction. MEK and its acetals accounted for 94 %o ft he products over these catalysts. Other strong acids such as heteropoly acids have also demonstratedh igh conversion into MEK in the temperature range 150-180 8C. [11] The selectivity to MEK varied from 50 to 95 %, the main byproducts were the corresponding dioxolanes and 2-methylpropanal( MP). Zeolites were found to be active catalysts for transforming BDO into MEK in the temperature range 230-350 8C. [1f, 13-15] Differentz eolite structures, including FAU, MFI, MOR, and BEA, have also been tested. Whereas medium-pore zeolites favored MEK formation, the large-pore zeolitesy ielded acetals andk etals. [13] The selective synthesis of 3B2OL and BD from BDO has been achieved over less acidic solids and at higher temperatures. [8b, 16, 17] The first attempts for 3B2OL and BD synthesis go back to the 1940s.W infield [16] studied al arge number of metal oxidesa nd salts and found that the best catalyst for 3B2OL and BD production is ThO 2 .T hey disclosed that over ThO 2 , 3B2OL can be obtained with ay ield of 70 %a t3 50 8C, whereas 62 %y ield of BD can be reached at 500 8C. [16] Significant contribution to the synthesis of 3B2OL and BD from BDO has been madeb yt he group of Sato et al. [8b, 17, 18] during the few last years. These authors have investigated BDO dehydration over al arge range of rare-earth-oxidec atalysts. They disclosedt hat Sc 2 O 3 calcined at 800 8Cs hows excellent catalytic activity at 325 8Cw ith 85 %s electivity to 3B2OL at 2,3-Butanediol (BDO) is an excellent feedstock for expanding the network of bio-based chemicals through catalytic technologies. The dehydration of BDO provides an alternative green route to important chemicals such as methyl ethyl ketone (MEK) and1 ,3-butadiene (BD). In this contribution, we report on the catalytic performanceo fb oron (BP), aluminum (AlP), titanium (TiP), zirconium (ZrP), and niobium (NbP) phosphates in BDO dehydration.T he kinetic study points to three reaction pathways operating over phosphate catalysts, leading to MEK, 2-methyl-propanal( MP), and BD via intermediate 3-butene-2-ol (3B2OL) formation. The major reaction pathway is shownt oi nvolve pinacolr earrangementt oM EK. The reactivity of phosphates is found to increasei nt he order:B P< TiP < ZrP = NbP < AlP.T he best catalytic performance is achieved over Al...

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Enhancement of 2,3‐Butanediol Production by Klebsiella oxytoca PTCC 1402

Cited by 16 publications

References 19 publications

Analysis of acetic acid, furfural and 5‐hydroxymethylfurfural affecting 2, 3‐butanediol production using Klebsiella oxytoca

Analysis of acetic acid, furfural and 5‐hydroxymethylfurfural affecting 2, 3‐butanediol production using Klebsiella oxytoca

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

Dehydration of 2,3-butanediol over zeolite catalysts

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