Enhancement of anthraquinone production and release by combination of culture medium selection and methyl jasmonate elicitation in hairy root cultures of Rubia tinctorum

Perassolo, Marı́a; Cardillo, Alejandra B.; Mugas, María Laura; Montoya, Susana C. Núñez; Giulietti, Ana María; Talou, Julián Rodríguez

doi:10.1016/j.indcrop.2017.05.010

Cited by 48 publications

(17 citation statements)

References 44 publications

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“…Some examples include Agave salmiana for saponins (Puente-Garza et al 2017a), Rhaponticoide mykalea for chlorogenic acids (Hayta et al 2017) and phenolic compounds (Karalija et al 2017), Leucophyllum frutescens for phenolic compounds (Espinosa-Leal et al 2015) and Poliomintha glabrescens for luteolin (García-Pérez et al 2012). All of these compounds are useful in the food, pharmaceutical or cosmetic industries (Perassolo et al 2017). Other crop plants are used for the production of metabolites such as betalains, which are natural colorants as well as good antioxidants.…”

Section: Plants Employed For the Production Of Biologically Active Comentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

434

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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Section: Plants Employed For the Production Of Biologically Active Comentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

434

206

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“…use of transgenic or nontransgenic HRCs), the production of specialized metabolites has been successfully improved by optimizing the growth conditions (e.g. carbon source, aeration, pH, light/dark, culture medium composition), selection of the clone (Sevón et al, 1998), and/or by paying a particular attention to the selection of adapted elicitors (Wang and Wu, 2013;Perassolo et al, 2017;Halder et al, 2018). The expression of specialized metabolites in HRCs, as in all plant-based production systems, requires the identification of the most appropriate elicitors and administration scheme.…”

Section: Production Of Specialized Metabolitesmentioning

confidence: 99%

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Gutierrez-Valdes

Häkkinen

Lemasson³

et al. 2020

Front. Plant Sci.

188

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Hairy roots derived from the infection of a plant by Rhizobium rhizogenes (previously referred to as Agrobacterium rhizogenes) bacteria, can be obtained from a wide variety of plants and allow the production of highly diverse molecules. Hairy roots are able to produce and secrete complex active glycoproteins from a large spectrum of organisms. They are also adequate to express plant natural biosynthesis pathways required to produce specialized metabolites and can benefit from the new genetic tools available to facilitate an optimized production of tailor-made molecules. This adaptability has positioned hairy root platforms as major biotechnological tools. Researchers and industries have contributed to their advancement, which represents new alternatives from classical systems to produce complex molecules. Now these expression systems are ready to be used by different industries like pharmaceutical, cosmetics, and food sectors due to the development of fully controlled large-scale bioreactors. This review aims to describe the evolution of hairy root generation and culture methods and to highlight the possibilities offered by hairy roots in terms of feasibility and perspectives.

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“…JA, MJ, SA, and ASA have been recognized as another class of signal transducer in elicitation of plant defense responses [30,42,[65][66][67][68][69][70][71][72][73][74]. Application of MJ, JA, SA, ASA, and other signal molecules may or may not inhibit growth and thus effect biomass accumulation during the enhancement of SMs production or vice-versa [8,42].…”

Section: Effect Of Signal Molecules On Sm Productionmentioning

confidence: 99%

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

238

116

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Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures. Secondary metabolite accumulation in vitro or their efflux in culture medium has been elicited in the undifferentiated or differentiated tissue cultures of several plant species by the application of a low concentration of biotic and abiotic elicitors in the last three decades. Hairy root cultures are preferred for the application of elicitation due to their genetic and biosynthetic stability, high growth rate in growth regulator‐free media, and production consistence in response to elicitor treatment. Elicitors act as signal, recognized by elicitor‐specific receptors on the plant cell membrane and stimulate defense responses during elicitation resulting in increased synthesis and accumulation of secondary metabolites. Optimization of various parameters, such as elicitor type, concentration, duration of exposure, and treatment schedule is essential for the effectiveness of the elicitation strategies. Combined application of different elicitors, integration of precursor feeding, or replenishment of medium or in situ product recovery from the roots/liquid medium with the elicitor treatment have showed improved accumulation of secondary metabolites due to their synergistic effect. This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.

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Enhancement of anthraquinone production and release by combination of culture medium selection and methyl jasmonate elicitation in hairy root cultures of Rubia tinctorum

Cited by 48 publications

References 44 publications

In vitro plant tissue culture: means for production of biological active compounds

In vitro plant tissue culture: means for production of biological active compounds

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

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