Enhancement of antibacterial activity by a copper(II) and zinc(II) in chelation with ethylenediaminetetra-acetic acid and urea complex

Effects of size on silver nanoparticle stability and inhibition of Vibro parahaemolyticus bacterial cell growth in high chloride media

“…The potential values of all PVP-coated AgNP samples were nearly similar, from −10 mV to −12.7 mV. However, their standard deviations were decreased with correlation to the sizes of AgNPs [9]. The surface plasmon resonance spectra of all these PVP-AgNP samples diluted in DI water at 20 ppm are presented in figure 2.…”

“…The disc diffusion method was used to test the effects of the AgNPs at several concentrations at 1000 ppm(G), 100 ppm(−1), 10 ppm(−2), and 1ppm (−3) on the survival of Vibrio parahaemolyticus bacteria, following the Kirby-Bauer disc diffusion susceptibility test protocol with slight modification [13], as follows: the agar plates were prepared by autoclaving 3% NaCl-LB agar at 121 °C for 15 min and poured into 10 cm diameter Petri dishes at 20 ml/plate. The agar plate preparation and experimental design are similarly performed as described in our previous report [9]. Briefly, each AgNP size was repeated with three plates at each time point in 3 repeated experiments.…”

“…Numerous studies have shown how metal ions and nanostructure activity can destroy bacterial cells and inhibit their ability to replicate DNA. These mechanisms include (1) the uptake of metal ion into cells, which results in reducing the production of intracellular ATP and disruption of DNA replication; (2) metal ion release and reactive oxygen species (ROS) generation; and (3) metal nanoparticle accumulation and dissolution in the bacterial membrane [8,9]. Silver transition metal has strong antibacterial properties and is widely used as a chemotherapy agent to prevent and treat infections.…”