Enhancement of Cellulose Degradation Potential of Bacillus sp. Hcb-21 through Mutagenesis

Rk, Bhatia; Kumar, Ravindra; Rathour, Ranju Kumari; Kumar, Yatendra; Sharma, Vishal; Rana, Nidhi; Thakur, Shikha; Ak, Bhatt

doi:10.4172/1948-5948.1000374

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…However, the potential utilization of this waste for valuable products, particularly bioethanol, is under investigation due to its wide availability, renewability, and costeffectiveness (Tura et al, 2018;Rathour et al, 2023). Production of bioethanol from tetra pack cellulose involves several steps, including pretreatment, enzymatic hydrolysis, microbial fermentation, and distillation, making it a viable biofuel alternative to petrol (Bhatia et al, 2017;Bhatt et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Upcycling of tetra pack waste cellulose into reducing sugars for bioethanol production using Saccharomyces cerevisiae

Rinki,

Yadav,

Sharma

et al. 2024

Preprint

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Bioethanol production from waste materials offers a promising avenue for sustainable energy and waste management. In this study, fermentable sugars derived from tetra pack waste cellulose were biotransformed into bioethanol using Saccharomyces cerevisiae. Tetra pack waste (180 g) yielded tetra pack cellulosic pulp (TPCP) of 145 g, after removing the different layers representing 80.56±0.32% of the original weight. Cellulase from Bacillus sp. RL-07, with a cellulolytic potential of 6.98±0.36 U/ml, released 32.72±0.12 mg/ml of reducing sugars, achieving 44.60±0.56% saccharification of TPCP under optimized conditions. Subsequent fermentation of the broth (1 L) with tetra pack cellulosic pulp hydrolysate (TPCPH) (50% v/v), containing 5.12 g of reducing sugars, by S. cerevisiaeyielded 1.42 g of bioethanol per g of reducing sugars under optimized conditions, with a volume productivity of 0.24 g/l/h and a purity of 96.42% was confirmed by GC/MS analysis. The findings of this research demonstrated the potential of tetra pack waste for bioethanol production, contributing to sustainable waste management, addressing energy shortages, and mitigating environmental pollution, thereby supporting goals of sustainable growth and development.

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