Enhancement of chondrogenic differentiation potential of equine adiposetissue-derived mesenchymal stem cells using TGF-β3 and BMP-6

Shademan, Milad; Parham, Abbas; Dehghani, Hesam

doi:10.3906/biy-1501-61

Cited by 6 publications

(6 citation statements)

References 30 publications

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“…In addition, we did not observe the lacuna formation typical for hyaline cartilage. This finding was in contrast to the results documented by Shademan et al (2015), who recorded lacunae in their pellets. They used pellet culture system with more complex chondrogenic medium consisted of dexamethasone, ascorbic acid, ITS, TGF-β3 and BMP-6.…”

Section: Discussioncontrasting

confidence: 99%

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Comparative analysis of mesenchymal stromal cells from different tissue sources in respect to articular cartilage tissue engineering

Danišovič¹,

Boháč²,

Zamborský³

et al. 2016

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The main goal of this study was a comparison of biological properties of mesenchymal stromal cells (MSCs) obtained from bone marrow, adipose tissue and umbilical cord with respect to articular cartilage regeneration. MSCs were isolated and expanded in vitro up to the third passage. The kinetics of proliferation was analyzed by cell analyzer CEDEX XS and expression of selected markers was assessed by flow cytometry. The morphology was analyzed by inverted microscope and TEM. Pellet culture system and chondrogenic medium containing TGF-β1 was used to induce chondrogenic differentiation. Chondrogenesis was analyzed by real-time PCR; the expression of collagen type I and type II was compared. MSCs from all sources showed similar kinetics of proliferation and shared expression of CD73, CD90 and CD105; and were negative for CD14, CD20, CD34 and CD45. Observation under inverted microscope and TEM showed similar morphology of all analyzed MSCs. Cells from all sources underwent chondrogenic differentiation - they expressed collagen type II and acid mucopolysaccharides typical for hyaline cartilage. On the basis of obtained results it should be emphasized that MSCs from bone marrow, adipose tissue and umbilical cord share biological properties. They possess the chondrogenic potential and may be utilized in cartilage tissue engineering.

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Section: Discussioncontrasting

confidence: 99%

“…It provides 3D architecture of tissue in which the cells are in deep contact and may influence each other by paracrine signaling (Mara et al 2010). This effect is also additively enhanced by supplementation of growth factors such as TGF-β1, TGF-β3, BMP-2, BMP-6 and GDF-5 (Murphy et al 2015;Shademan et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of mesenchymal stromal cells from different tissue sources in respect to articular cartilage tissue engineering

Danišovič¹,

Boháč²,

Zamborský³

et al. 2016

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“…Chondrogenic differentiation of MSCs has been described using different induction mediums containing e.g. transforming growth factor β 3 (TGF-β 3 ) and BMP6 [ 56 , 57 ]. Selection of chondrogenic induction medium containing TGF-β 1 and dexamethasone for the present study was based on large, high-throughput mRNA profiling studies, showing this cocktail enhanced expression of hyaline articular cartilage markers, while downregulating unwanted pro-osteogenic markers [ 19 , 58 ].…”

Section: Methodological Considerationsmentioning

confidence: 99%

Donor age effects on in vitro chondrogenic and osteogenic differentiation performance of equine bone marrow- and adipose tissue-derived mesenchymal stromal cells

et al. 2022

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Background Bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stromal cells (MSCs) have shown potential as cell-based therapies for cartilage and bone injuries and are used increasingly in human and veterinary practice to facilitate the treatment of orthopedic conditions. However, human and rodent studies have documented a sharp decline in chondrogenic and osteogenic differentiation potential with increasing donor age, which may be problematic for the important demographic of older orthopedic patients. The aim of this study was to identify the effect of donor age on the chondrogenic and osteogenic differentiation performance of equine BM- and AT-MSCs in vitro. BM- and AT-MSCs and dermal fibroblasts (biological negative control) were harvested from horses in five different age groups (n = 4, N = 60); newborn (0 days), yearling (15–17 months), adult (5–8 years), middle-aged (12–18 years), and geriatric (≥ 22 years). Chondrogenic differentiation performance was assessed quantitatively by measuring pellet size, matrix proteoglycan levels, and gene expression of articular cartilage biomarkers. Osteogenic differentiation performance was assessed quantitatively by measuring alkaline phosphatase activity, calcium deposition, and gene expression of bone biomarkers. Results Chondrogenic and osteogenic differentiation performance of equine BM- and AT-MSCs declined with increasing donor age. BM-MSCs had a higher chondrogenic differentiation performance. AT-MSCs showed minimal chondrogenic differentiation performance in all age groups. For osteogenesis, alkaline phosphatase activity was also higher in BM-MSCs, but BM-MSCs calcium deposition was affected by donor age earlier than AT-MSCs. Chondrogenic and osteogenic differentiation performance of BM-MSCs exhibited a decline as early as between the newborn and yearling samples. Steady state levels of mRNA encoding growth factors, chondrogenic, and osteogenic biomarkers were lower with increasing donor age in both MSC types. Conclusions The data showed that chondrogenic and osteogenic differentiation performance of equine BM-MSCs declined already in yearlings, and that AT-MSCs showed minimal chondrogenic potential, but were affected later by donor age with regards to osteogenesis (calcium deposition). The results highlight the importance of donor age considerations and MSC selection for cell-based treatment of orthopedic injuries and will help inform clinicians on when to implement or potentially cryopreserve cells. Moreover, the study provides molecular targets affected by donor age.

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“…In the triangle of tissue engineering, in addition to having a suitable cell source and an ideal scaffold, culture medium has an important consequence on tissue regeneration, especially in inducing cellular differentiation (Sheykhhasan, Qomi, Kalhor, Mehdizadeh, & Ghiasi, ). Previous studies in cartilage regeneration have shown that chondro‐inductive factors such as fibroblast growth factor‐2 (Hildner et al, ; Ito, Sawada, Fujiwara, & Tsuchiya, ), insulin‐like growth factor‐1 (Jaklenec et al, ), platelet‐derived growth factor (PDGF; Kang et al, ; Ng et al, ), bone morphogenetic protein‐6 (BMP‐6; Cheng, Gustafson, Tooley, & Zhang, ; Shademan, Parham, & Dehghani, ), and transforming growth factor beta‐3 (TGF‐β3; Almeida et al, ; Shademan et al, ) play an important role in differentiation of MSCs into chondrocytes. Alpha granules present in platelet‐rich plasma (PRP), containing high amounts of TGF‐β3 and other growth factors, are widely used to treat osteoarthritis (Okuda et al, ).…”

Section: Introductionmentioning

confidence: 99%

Activated platelet-rich plasma improves cartilage regeneration using adipose stem cells encapsulated in a 3D alginate scaffold

Beigi

Atefi

Ghanaei

et al. 2018

J Tissue Eng Regen Med

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In the current study, the effect of superimposing platelet-rich plasma (PRP) on different culture mediums in a three-dimensional alginate scaffold encapsulated with adipose-derived mesenchymal stem cells for cartilage tissue repair is reported. The three-dimensional alginate scaffolds with co-administration of PRP and/or chondrogenic supplements had a significant effect on the differentiation of adipose mesenchymal stem cells into mature cartilage, as assessed by an evaluation of the expression of cartilage-related markers of Sox9, collagen II, aggrecan and collagen, and glycosaminoglycan assays. For in vivo studies, following induction of osteochondral lesion in a rabbit model, a high degree of tissue regeneration in the alginate plus cell group (treated with PRP plus chondrogenic medium) compared with other groups of cell-free alginate and untreated groups (control) were observed. After 8 weeks, in the alginate plus cell group, functional chondrocytes were observed, which produced immature matrix, and by 16 weeks, the matrix and hyaline-like cartilage became completely homogeneous and integrated with the natural surrounding cartilage in the defect site. Similar effect was also observed in the subchondral bone. The cell-free scaffolds formed fibrocartilage tissue, and the untreated group did not form a continuous cartilage over the defect by 16 weeks.

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Enhancement of chondrogenic differentiation potential of equine adiposetissue-derived mesenchymal stem cells using TGF-β3 and BMP-6

Cited by 6 publications

References 30 publications

Comparative analysis of mesenchymal stromal cells from different tissue sources in respect to articular cartilage tissue engineering

Comparative analysis of mesenchymal stromal cells from different tissue sources in respect to articular cartilage tissue engineering

Donor age effects on in vitro chondrogenic and osteogenic differentiation performance of equine bone marrow- and adipose tissue-derived mesenchymal stromal cells

Activated platelet-rich plasma improves cartilage regeneration using adipose stem cells encapsulated in a 3D alginate scaffold

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