Enhancement of co-production of nutritional protein and carotenoids in Dunaliella salina using a two-phase cultivation assisted by nitrogen level and light intensity

Sui, Yixing; Muys, Maarten; Waal, Dedmer B. Van de; D’Adamo, Sarah; Vermeir, Pieter; Fernandes, Tânia V.; Vlaeminck, Siegfried E.

doi:10.1016/j.biortech.2019.121398

Cited by 59 publications

(45 citation statements)

References 46 publications

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“…Dunaliella, yet they can also lower the light usage efficiency by microalgae, resulting in lower protein yield [5,7,8]. Lastly, the growth phase of microalgae can affect the protein dynamics significantly [4,5].…”

Section: Dunaliella Protein Tunability: Exploring the Spectrummentioning

confidence: 99%

“…Only recently, one study demonstrated that EAA levels in Dunaliella increase from the exponential towards the stationary phase, despite the increasedecrease pattern of protein quantity [5]. Another study shows a clear benefit of N limitation to boost EAA production in Dunaliella despite reduced protein quantity [8]. Both studies indicate the superior quality of Dunaliella protein by its essential amino acid index (EAAI) as high as 1.53 (Box 1).…”

Section: Dunaliella Protein Tunability: Exploring the Spectrummentioning

confidence: 99%

“…Consequently, a new approach to co-produce multiple products of interest (e.g. high quality protein and β-carotene together) in Dunaliella biomass may be more advantageous [8]. Similarly, a co-production of some functional proteins can further improve the quality of Dunaliella biomass.…”

Section: Future Outlooks and Concluding Remarksmentioning

confidence: 99%

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Dunaliella Microalgae for Nutritional Protein: An Undervalued Asset

Sui

Vlaeminck

2020

Trends in Biotechnology

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β-carotene production with Dunaliella microalgae is established, yet their potential as protein source for food and feed applications seems overlooked. The rich protein content and nutritional tunability of Dunaliella make these algae intriguing sources of sustainable protein. It is of societal interest to exploit these promising proteinaceous Dunaliella traits. Dunaliella microalgae: Spotlighted β-carotene and undervalued protein Microalgae are recognized as promising sources for diversified applications including food, feed and high-value products [1]. Both science and industry have already focused their interests on Dunaliella microalgae, especially for their unique feature of hyper carotenogenesis, producing

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Section: Dunaliella Protein Tunability: Exploring the Spectrummentioning

confidence: 99%

Section: Dunaliella Protein Tunability: Exploring the Spectrummentioning

confidence: 99%

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Dunaliella Microalgae for Nutritional Protein: An Undervalued Asset

Sui

Vlaeminck

2020

Trends in Biotechnology

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“…A specific marine microalgae species can be used as a feedstock for many different bio-compounds. For instance, Phaeodactylum tricornutum is a good candidate for fucoxanthin and EPA [12], Dunaliella salina can be used to produce proteins and carotenoids [13], and Spirulina sp. Can accumulate a large amount of carbonic anhydrase, C-phycocyanin, and allophycocyanin [14].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Wang

Chua

et al. 2020

Marine Drugs

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Marine microalgae are regarded as potential feedstock because of their multiple valuable compounds, including lipids, pigments, carbohydrates, and proteins. Some of these compounds exhibit attractive bioactivities, such as carotenoids, ω-3 polyunsaturated fatty acids, polysaccharides, and peptides. However, the production cost of bioactive compounds is quite high, due to the low contents in marine microalgae. Comprehensive utilization of marine microalgae for multiple compounds production instead of the sole product can be an efficient way to increase the economic feasibility of bioactive compounds production and improve the production efficiency. This paper discusses the metabolic network of marine microalgal compounds, and indicates their interaction in biosynthesis pathways. Furthermore, potential applications of co-production of multiple compounds under various cultivation conditions by shifting metabolic flux are discussed, and cultivation strategies based on environmental and/or nutrient conditions are proposed to improve the co-production. Moreover, biorefinery techniques for the integral use of microalgal biomass are summarized. These techniques include the co-extraction of multiple bioactive compounds from marine microalgae by conventional methods, super/subcritical fluids, and ionic liquids, as well as direct utilization and biochemical or thermochemical conversion of microalgal residues. Overall, this review sheds light on the potential of the comprehensive utilization of marine microalgae for improving bioeconomy in practical industrial application.

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“…Dentro de los organismos que son aprovechados por la acuacultura para la producción de biocompuestos se encuentran las microalgas. Estos microorganismos son capaces de sintetizar compuestos bioactivos como los antioxidantes, que pueden ser utilizados como complementos alimenticios (Sui et al, 2019). Diversos estudios han reportado que la manipulación de las condiciones del cultivo de ciertas especies de microalgas favorecen la producción de compuestos orgánicos de interés (Rao et al, 2006;Yeesang y Cheirsilp, 2011;Yeh y Chang, 2012), tales como los carotenos, ficobilinas, xantofilas, entre otros (Rema y Gouveia, 2005;Yaşar y Şevket, 2006;Abd El-Baky et al, 2007).…”

Section: Introductionunclassified

Efecto del estrés por nitrógeno y salinidad en el contenido de b-caroteno de la microalga Dunaliella tertiolecta//Effect of nitrogen and salinity stress on the β-carotene content of the microalgae Dunaliella tertiolecta

et al. 2020

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Las microalgas del género Dunaliella son cultivadas con la finalidad de obtener compuestos antioxidantes, principalmente carotenoides. En este estudio se analizó el crecimiento, biomasa y contenido de β-caroteno de Dunaliella tertiolecta cultivada en condiciones de estrés por nitrógeno y salinidad. Se comparó el efecto de la salinidad a 35, 45 y 55 UPS, cada una en combinación con los medios F, F/8 y F/16; como control se usó el medio F a 35 UPS. Se utilizó un diseño factorial 3x3 a nivel de matraces de 250 mL. Se realizaron conteos celulares para determinar la fase de crecimiento estacionaria, en la cual se tomaron muestras para realizar el análisis proximal y determinar el contenido de β-caroteno. Se observó que la limitación de nitrógeno en el medio de cultivo afecta negativamente la densidad celular, sin embargo, la concentración de biomasa y materia orgánica tienden a incrementar, no siendo así en el contenido de β-caroteno. Por otro lado, el aumento en la salinidad favoreció el incremento de este pigmento. Por lo anterior, se concluyó que las condiciones más adecuadas para la obtención de β-caroteno, es el medio F a 45 UPS a partir del cual se obtuvieron rendimientos aproximados de 90 mg L-1.ABSTRACTThe Dunaliella genus is cultivated to obtain antioxidant compounds, mainly carotenoids. In this study we analyzed growth, biomass and β-carotene content of Dunaliella tertiolecta cultivated under nitrogen and salinity stress. The salinity effects at 35, 45 and 55 UPS were compared, each one in combination with the F, F/8 and F/16 media; the F medium at 35 UPS was utilized as control. The experiment was carried out by the implementation of a 3x3 factorial design, using 250 mL flasks. Cell counts were done to determine the stationary growth phase, in which samples were taken for proximal analysis and determine the β-carotene content. It was observed that nitrogen limitation in culture media causes a negative effect on the cellular density and the β-carotene content; however, biomass and organic matter augmented under these conditions. On the other hand, the increase in salinity promoted high concentrations of the pigment. Based on the above findings, it was concluded that the most suitable conditions for the β-carotene production, is F medium at 45 UPS with yields of approximately 90 mg L-1.

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Enhancement of co-production of nutritional protein and carotenoids in Dunaliella salina using a two-phase cultivation assisted by nitrogen level and light intensity

Cited by 59 publications

References 46 publications

Dunaliella Microalgae for Nutritional Protein: An Undervalued Asset

Dunaliella Microalgae for Nutritional Protein: An Undervalued Asset

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Efecto del estrés por nitrógeno y salinidad en el contenido de b-caroteno de la microalga Dunaliella tertiolecta//Effect of nitrogen and salinity stress on the β-carotene content of the microalgae Dunaliella tertiolecta

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