Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture

Toyofuku, Miwako; Okutani, Fuki; Nakayasu, Masaru; Hamamoto, Shoichiro; Takase, Haruhiko; Yazaki, Kazufumi; Sugiyama, Akifumi

doi:10.1093/bbb/zbab017

Cited by 9 publications

(9 citation statements)

References 25 publications

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“…UV-B radiation can accelerate the conversion between malonyl-glycosidic and glycosidic isoflavone ( 25 ). Daidzein and genistein, which could induce nod gene to activate nodulation process ( 26 ), were related to the regulation of bacterial communities in soybean root, so their contents were lower in soybean hypocotyl and cotyledon calluses ( Table 1 ). Hence, the response of soybean hypocotyl and cotyledon calluses to UV-B radiation was different.…”

Section: Discussionmentioning

confidence: 99%

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

et al. 2023

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Isoflavones are a class of flavonoids that belong to a large family of polyphenols and synthesized predominantly in legume, and they play important roles including acting as antioxidant, preventing osteoporosis, reducing the risk of atherosclerosis, and protecting against cardiovascular disease. This study focused on the accumulation and synthetic metabolism of isoflavone in soybean hypocotyl and cotyledon calluses under UV-B radiation. The results showed that UV-B radiation significantly up-regulated the gene expression of phenylalanine ammonia lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), chalcone ketone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS), and enhanced their activity in soybean hypocotyl and cotyledon calluses. As a result, isoflavones content increased by 21.23 and 21.75% in soybean hypocotyl and cotyledon calluses, respectively. Among the isoflavones produced, malonyldaidzin was the dominant one in hypocotyl callus, while malonylglycitin and daidzein were the main isoflavones in cotyledon calluses. This study revealed that UV-B radiation induced isoflavone accumulation in soybean calluses, which could be an efficient strategy to improve the nutritional value of food and produce high levels of bioactive secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

et al. 2023

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“…In field-grown soybean, the secretion of daidzein is also higher in the early vegetative stages than in the reproductive stages, but the amount is increased up to 10,000-fold, compared with hydroponic conditions (Toyofuku et al 2021) (Figure 3). The increase of secretion is possibly due to the presence of microbes in the field; however, soil particles in the field may influence the root morphology and the rate of isoflavone secretion because the particle size and chemistry of growth substrates such as sand, clay, and glass beads, affect root morphology and exudation in Brachypodium distachyon (Sasse et al 2020).…”

Section: Secretion Into the Rhizospherementioning

confidence: 97%

“…Different classes of flavonoids, including flavones, flavonols, flavanones, isoflavones, and chalcones, have since been identified as plant signals for induction of nod genes in the rhizobia in addition to non-flavonoid metabolites (Janczarek et al 2015;Liu and Murray 2016). Most of these flavonoids induce nod genes at low micromolar concentrations within the range of the rhizospheric concentrations, at least for daidzein (Sugiyama et al 2017;Toyofuku et al 2021). The specificity of flavonoid profiles in each legume plant and the specific perception of flavonoids by NodD, a LysR-type transcription regulator, in the rhizobia are responsible for the first level of host specificity for legume-rhizobia symbiosis.…”

Section: Roles As Nod Gene Inducersmentioning

confidence: 99%

“…Traditionally, the amount of PSMs secreted from roots has been analyzed using hydroponic cultures because of the ease of applicability compared with field sampling (Oburger and Jones 2018). Although research in our laboratory has revealed that the secretion of daidzein from roots follows a similar pattern during the growth stages (i.e., higher secretion in early growth stages than later growth stages), the amount of daidzein secreted from roots was much higher in the field (Toyofuku et al 2021), pointing toward the importance of measurement of metabolites in field-grown plants. Along with the differences in the amount and rate of secretion, it is technically challenging to analyze the positional secretion rate using a hydroponic system.…”

Section: Dynamics Of Flavonoids and Saponins In The Rhizospherementioning

confidence: 99%

“…When the daidzein secretion rate measured in field-grown soybean was used for the simulation, the estimated daidzein concentration in the soybean rhizosphere was within the range of that from the rhizosphere in the soybean field (Toyofuku et al 2021). So far, the adsorption coefficient has been analyzed for flavonoids and saponins in addition to organic xenobiotics and estrogens in soil (Shaw and Hooker 2008;Caron et al 2010) (Fujimatsu et al 2020); thus, simulations based on a fluid model can be applied to predict the rhizosphere distribution of PSMs.…”

Section: Simulationmentioning

confidence: 99%

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Flavonoids and saponins in plant rhizospheres: roles, dynamics, and the potential for agriculture

Sugiyama

2021

Bioscience, Biotechnology, and Biochemistry

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Plants are in constant interaction with a myriad of soil microorganisms in the rhizosphere, an area of soil in close contact with plant roots. Recent research has highlighted the importance of plant specialized metabolites (PSMs) in shaping and modulating the rhizosphere microbiota; however, the molecular mechanisms underlying the establishment and function of the microbiota mostly remain unaddressed. Flavonoids and saponins are a group of PSMs whose biosynthetic pathways have largely been revealed. Although these PSMs are abundantly secreted into the rhizosphere and exert various functions, the secretion mechanisms have not been clarified. This review summarizes the roles of flavonoids and saponins in the rhizosphere with a special focus on interactions between plants and the rhizosphere microbiota. Furthermore, this review introduces recent advancements in the dynamics of these metabolites in the rhizosphere and indicates potential applications of PSMs for crop production and discusses perspectives in this emerging research field.

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α‐Tomatine gradient across artificial roots recreates the recruitment of tomato root‐associated Sphingobium

Takamatsu,

Toyofuku,

Okutani

et al. 2023

Plant Direct

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Abstractα‐Tomatine is a major saponin that accumulates in tomatoes (Solanum lycopersicum). We previously reported that α‐tomatine secreted from tomato roots modulates root‐associated bacterial communities, particularly by enriching the abundance of Sphingobium belonging to the family Sphingomonadaceae. To further characterize the α‐tomatine‐mediated interactions between tomato plants and soil bacterial microbiota, we first cultivated tomato plants in pots containing different microbial inoculants originating from three field soils. Four bacterial genera, namely, Sphingobium, Bradyrhizobium, Cupriavidus, and Rhizobacter, were found to be commonly enriched in tomato root‐associated bacterial communities. We constructed a pseudo‐rhizosphere system using a mullite ceramic tube as an artificial root to investigate the influence of α‐tomatine in modifying bacterial communities. The addition of α‐tomatine from the artificial root resulted in the formation of a concentration gradient of α‐tomatine that mimicked the tomato rhizosphere, and distinctive bacterial communities were observed in the soil close to the artificial root. Sphingobium was enriched according to the α‐tomatine concentration gradient, whereas Bradyrhizobium, Cupriavidus, and Rhizobacter were not enriched in α‐tomatine‐treated soil. The tomato root‐associated bacterial communities were similar to the soil bacterial communities in the vicinity of artificial root‐secreting exudates; however, hierarchical cluster analysis revealed a distinction between root‐associated and pseudo‐rhizosphere bacterial communities. These results suggest that the pseudo‐rhizosphere device at least partially creates a rhizosphere environment in which α‐tomatine enhances the abundance of Sphingobium in the vicinity of the root. Enrichment of Sphingobium in the tomato rhizosphere was also apparent in publicly available microbiota data, further supporting the tight association between tomato roots and Sphingobium mediated by α‐tomatine.

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Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture

Cited by 9 publications

References 25 publications

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

Flavonoids and saponins in plant rhizospheres: roles, dynamics, and the potential for agriculture

α‐Tomatine gradient across artificial roots recreates the recruitment of tomato root‐associated Sphingobium

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