Enhancement of dielectrophoresis using fractal gold nanostructured electrodes

Koklu, Anil; Sabuncu, Ahmet C.; Beşkök, Ali

doi:10.1002/elps.201600456

Cited by 20 publications

(19 citation statements)

References 53 publications

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“…This requires development of a reliable equivalent circuit model for the device. In addition, decreasing EP effects will increase device sensitivity in the low-frequency region, which can be achieved by using fractal electrodes, as previously shown by our group for a simple parallel and planar electrode devices [49,54].…”

Section: Discussionmentioning

confidence: 68%

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

et al. 2017

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Dielectric spectroscopy (DS) is a non-invasive, label-free, fast, and promising technique for measuring dielectric properties of biological cells in real time. We demonstrate a microchip that consists of electro-activated micro-well arrays for positive dielectrophoresis (pDEP) assisted cell capture, DS measurements, and negative dielectrophoresis (nDEP) driven cell unloading; thus, providing a high throughput cell analysis platform. To the best of our knowledge, this is the first microfluidic chip that combines electro-activated micro-wells and DS to analyze biological cells. Device performance is tested using Saccharomyces Cerevisiae (yeast) cells. DEP response of yeast cells is determined by measuring their Clausius-Mossotti (CM) factor using biophysical models in parallel plate micro-electrode geometry. This information is used to determine the excitation frequency to load and unload wells. Effect of yeast cells on the measured impedance spectrum was examined both experimentally and numerically. Good match between the numerical and experimental results establishes the potential use of the micro-chip device for extracting sub-cellular properties of biological cells in a rapid and non-expensive manner.

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Section: Discussionmentioning

confidence: 68%

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

et al. 2017

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“…Since the first report of the microfabrication technique for DEP devices in 1988, the 1990s saw an explosion of implementing DEP experiments with metal microelectrodes. The microelectrodes commonly used are carbon and metals, such as gold , platinum , titanium or silicon , among which gold and platinum electrodes are ideal choices due to the stable electrochemical property.…”

Section: Configurations Of Electrodes and Dep Devicesmentioning

confidence: 99%

Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

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Recent advances in dielectrophoresis-based cell viability assessmentDielectrophoresis (DEP) is a non-destructive, accurate, and label-free cell manipulating technique and DEP applications have been found in various fields. Assessment of cell viability is one of the important applications and many investigations have been reported. In this paper, cell polarization and its modeling, some key parameters employed for living/dead cell separation, as well as electrode configurations are reviewed. Focus is given to the latest development of DEP devices employed for the assessment of cell viability. Experimentally determined factors for separating living/dead cells, such as the conductivity of suspending medium and the frequency of applied electric field, are summarized. The future directions and potential challenges in this field are also outlined. Theory of cell DEPThe fundamentals of DEP were systematically reviewed in the past [35,36]. In this section, we only focus on the theory concerning cellular DEP. Based on the spherical particle model, single-shell model and multi-shell model of biological cells are extended. Color online: See the article online to view Figs. 1-7 in color. J. Zhang et al. Electrophoresis 2020, 41, 917-932

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“…As an example, Figure S1 shows the error estimation in each refinement step at 1 MHz frequency for a case with H/r = 4.5 and R/r = 2.5. In a typical DS experiment, the measured cell impedance spectra is fitted to a combination of a mixture model to represent cell suspension and constant phase element (CPE) model, which is a widely used empirical model to represent the impedance at the electrode/electrolyte interface [37][38][39]. In this study, the simulated cell suspension impedance spectrum was combined in series with a CPE element, to represent experimental data, where electrode polarisation is present.…”

Section: Forward Modelmentioning

confidence: 99%

Accuracy of the Maxwell–Wagner and the Bruggeman–Hanai mixture models for single cell dielectric spectroscopy

Mansoorifar

Ghosh

Sabuncu

et al. 2017

IET nanobiotechnol.

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Dielectric spectroscopy (DS) is a non-invasive, label-free, and promising technique for measuring dielectric properties of biological cells. Recent developments in microfabrication techniques made it possible to perform DS measurements with minute volume of cell suspensions. However, when the cell size approaches the size of the measurement chamber, especially, for single cell measurements, the analytical models [Maxwell-Wagner and Bruggeman-Hanai (BH) mixture models] to extract cell parameters lose their accuracy. Moreover, variations in the cell position relative to the measurement electrodes decrease the accuracy of the analytical solutions. Impedance spectrum of a typical eukaryotic mammalian cell is generated for different geometrical configurations using finite element. The generated data are fitted to the analytical models and extracted cell parameters are compared with the original values. The results show that BH model works more effectively when chamber to cell radius ratio is <3.5 and chamber height to cell radius ratio is <3. Moreover, it is observed that analytical models estimate cell parameters with major errors when the cells are in the vicinity of the electrodes. However, for high-volume fraction simulations, the BH model was able to predict cell parameters better even in the vicinity of the electrodes.

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Enhancement of dielectrophoresis using fractal gold nanostructured electrodes

Cited by 20 publications

References 53 publications

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

Recent advances in dielectrophoresis‐based cell viability assessment

Accuracy of the Maxwell–Wagner and the Bruggeman–Hanai mixture models for single cell dielectric spectroscopy

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