1992
DOI: 10.1016/0003-2697(92)90239-4
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Enhancement of firefly luciferase activity by cytidine nucleotides

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Cited by 33 publications
(16 citation statements)
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“…The stimulation of the post-flash light production by CoA [10] and CTP [11] has demonstrated the regulatory function of nucleotides. Thus binding of nucleotides to the E-P complex may induce conformational changes that promote the product release.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The stimulation of the post-flash light production by CoA [10] and CTP [11] has demonstrated the regulatory function of nucleotides. Thus binding of nucleotides to the E-P complex may induce conformational changes that promote the product release.…”
Section: Discussionmentioning
confidence: 99%
“…The use of stimulatory substances has its advantage mostly in increasing the sensitivity of bioluminometric assays when using the firefly luciferase gene as a reporter [11]. Thus neither high luc concentrations nor the use of allosteric regulators are appropriate means of increasing light production during ATP monitoring.…”
Section: Discussionmentioning
confidence: 99%
“…For the quantification of ATP Adenosine 5'-triphosphate (ATP) Bioluminescent Assay Kit [23,24] was used. In the ATP Bioluminescent Assay, ATP is consumed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin.…”
Section: Quantitative Measurement Of Extra Cellular Atpmentioning
confidence: 99%
“…This reaction can be extended to produce a "stable" luminescence plateau of 30-to 60-s duration by including coenzyme A in the reaction mix (Wood 1991;Ford et al 1992). Luciferase extractive assay methods have been described in detail previously (Millar et al 1992;Mankin et al 1997), so only general principles will be covered here.…”
Section: Measuring Luciferase Activity In Plant Extractsmentioning
confidence: 99%