Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids

Vaschenko, S. V.; Ramanenka, A. A.; Кулакович, О. С.; Muravitskaya, Alina; Гузатов, Д. В.; Lunevich, Anatoly Ya.; Glukhov, Yuri F.; Гапоненко, С. В.

doi:10.1016/j.proeng.2015.08.1111

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…Chemical properties of the glass surface are well‐established, so the deposition of metal nanoparticles onto glass is more reproducibly reliable . Silanization of the glass substrate or predeposition of a polyelectrolyte (PE) layer are used for colloidal nanoparticle immobilization. The latter is an easy technique based on electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

“…Plasmonic enhancement of fluorescence has become an active field of research during the last decades. It has numerous potential applications in analytical spectroscopy, nanobiosensors, fluorescence‐based applications of drug discovery, immunoassays, and light‐emitting devices . Fluorescent enhancement depends on different factors, such as size and shape of the metallic nanoparticles, its absorption properties, distance between particles and fluorophore and excitation/emission wavelengths …”

Section: Introductionmentioning

confidence: 99%

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Colloidal Silver Films on Polypropylene and Polyethylene

Muravitskaya

Кулакович

Adam

et al. 2018

Physica Status Solidi (b)

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A simple procedure for the fabrication of colloidal silver films on the surface of flexible polymer substrates, such as polypropylene and polyethylene, with the use of only polyelectrolyte pre‐treatment is presented. The optical properties of these films depend on the number of predeposited polyelectrolyte layers and salt concentration. The uncharged nature of the polymer surface allows to alternate the first polyelectrolyte layer (positively or negatively charged). The proposed method of silver deposition can be used for manufacturing silver films of different density on the polymer surfaces. This approach is low‐cost, does not require special equipment and allows to control the surface properties of the films. Using an average salt concentration (0.5 M) during the deposition of polyelectrolytes allowed to produce the densest films.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colloidal Silver Films on Polypropylene and Polyethylene

Muravitskaya

Кулакович

Adam

et al. 2018

Physica Status Solidi (b)

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“…The biomolecules were initially drop-coated onto PEM devices fabricated using all of the three techniques on Al-tape substrates. A constant incubation time of 60 min was maintained throughout the experiments. , The sticky and loosely bound protein molecules were removed by thoroughly washing with DI water after completion of the incubation time. The incubated substrates were further dried under an ultrahigh pure nitrogen blanket.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence Signal Enhancement by a Spray-Assisted Layer-by-Layer Technique on Aluminum Tape Devices for Biosensing Applications

Boranna

Nataraj²,

Nanjunda

et al. 2022

Langmuir

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Layer-by-layer (LbL) self-assembled polyelectrolyte multilayer (PEM) films are a simple yet elegant bottom-up technology to create films at the nano−microscale. This low-cost technology has been widely used as a universal functionalization technique on a broad spectrum of substrates. Biomolecules under investigation can be incubated onto films based on complementary charge interactions between the films and biomolecules. There is a great demand for developing an ultralow-cost biosensing device, which can optimally enhance the fluorescence signal of the adsorbed biomolecules from the traditional labeled sensing platforms. In this work, we have incorporated a blend of the conventional metal enhanced fluorescence technology and the PEM as a dielectric spacer and functionalized film, coated on an aluminum paper (tape)-based substrate. These device has been found to be capable of holding biomolecules in three-dimensional PEM space. The devices fabricated by the proposed spray LbL technique provide significant fluorescence signal enhancement by holding a relatively higher mass per volume of the adsorbed biomolecules, when compared to traditional spin-and dip-coating techniques. Interestingly, our proposed device has expressed a fluorescence enhancement factor, which is 9 times higher than PEM-functionalized glass-based devices. To demonstrate the practical utility of our devices, we also compared our devices to Whatman FAST slides. Our experimental fluorescence results are almost comparable to Whatman FAST slides. Such PEM devices fabricated on top of low-cost aluminum tape using a spray LbL technique give new insights into the future development of ultralow-cost, high-throughput, and disposable lab-on-chip diagnostic applications.

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“…Previously, we demonstrated the successful implementation of nanostructured silver films in the detection procedure of art materials 9,10 and bromates by SERS, 11 as well as in increasing sensitivity of fluorescence analysis of labeled proteins, 12 including antibodies to alpha-fetoprotein and prostate-specific antigen using plasmon-enhanced fluorescence. 13,14 In this regard, the aim of this work was to develop a sampling procedure and conditions for recording the SERS spectra of the benzimidazole (BIZ) fungicide carbendazim as well as to study the possible effect of the solvent on the SERS spectra of carbendazim. Standard methods for extracting pesticides from water, soil, and food involve the use of organic solvents because most pesticides are insoluble or slightly soluble in water.…”

Section: Introductionmentioning

confidence: 99%

Challenges in surface-enhanced Raman scattering detection of pesticide carbendazim and ways to overcome

2022

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.Considerable details in the sampling procedure for the detection of the pesticide carbendazim by surface-enhanced Raman scattering (SERS) were developed. Experimental parameters and recommendations were selected to minimize side processes of carbendazim photodestruction as well as to reduce the background signal from the silver substrate. It was found from the comparison of experimental and density functional theory calculations data that the position of the SERS bands in the carbendazim spectrum depends on the nature of the applied solvent due to different orientations of carbendazim with respect to the surface of silver nanoparticle film. Adsorption from a chloroform solution is preferable to form an aqueous solution for SERS because random adsorption of molecules on a silver surface provides robust carbendazim identification, displaying a larger number of bands in the SERS spectrum, a higher “signal-to-noise” ratio, and then the processes of carbendazim photodestruction slow down.

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Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids

Cited by 16 publications

References 39 publications

Colloidal Silver Films on Polypropylene and Polyethylene

Colloidal Silver Films on Polypropylene and Polyethylene

Fluorescence Signal Enhancement by a Spray-Assisted Layer-by-Layer Technique on Aluminum Tape Devices for Biosensing Applications

Challenges in surface-enhanced Raman scattering detection of pesticide carbendazim and ways to overcome

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