Enhancement of lysozyme stability and activity by polyamines

Powroznik, Brigitte; Gharbi, Myriam; Dandrifosse, Guy; Peulen, Olivier

doi:10.1016/j.biochi.2004.09.009

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…The inhibition of lysosomal marker enzymes may be largely due to membrane stabilizing property of polyamines. [18] Polyamines are known to exhibit antioxidant activity[19–21] and the same has been confirmed in our experiments too. It is likely that both antioxidant activity as well as a membrane stabilizing effect of polyamines might be contributing to their anti-inflammatory activity observed in different types of inflammation.…”

Section: Discussionsupporting

confidence: 86%

Polyamines: Potential anti-inflammatory agents and their possible mechanism of action

Lagishetty¹,

Naik²

2008

Indian J Pharmacol

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Objective:To evaluate the anti-inflammatory activity of exogenously administered polyamines on experimentally induced acute and chronic inflammation in wistar rats and to elucidate their possible mechanism of action.Materials and Methods:The in vivo anti-inflammatory activity of polyamines was studied using acute (carrageenin paw edema), sub-acute (cotton pellet granuloma) and chronic (Freund's adjuvant induced arthritis) models of inflammation. The biochemical parameters like liver lipid peroxides, SGOT and SGPT were also measured.Results:Polyamines exhibited significant anti-inflammatory activity in acute, sub-acute and chronic models of inflammation. Polyamines treatment inhibited the increase in lipid peroxides in liver and the serum concentration of marker enzymes (glutamate oxaloacetate transferase and glutamate pyruvate transferase) during inflammation.Conclusion:Polyamines possess anti-inflammatory activity in acute and chronic inflammation which can be attributed to their anti-oxidant and /or lysosomal stabilization properties.

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Section: Discussionsupporting

confidence: 86%

Polyamines: Potential anti-inflammatory agents and their possible mechanism of action

Lagishetty¹,

Naik²

2008

Indian J Pharmacol

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“…Moreover, the increase in cell proliferation during the repair phase of mucosal damage could cause an increase in the total polyamines. These polycations are also considered reliable markers of hyper-proliferation and their enhancement following the toxic gliadin effect, could be similar to that observed during other inflammatory processes of the intestine leading to a derangement of the intestinal mucosa [ 36 ].…”

Section: Discussionmentioning

confidence: 81%

Lactobacillus rhamnosus GG Protects the Epithelial Barrier of Wistar Rats from the Pepsin-Trypsin-Digested Gliadin (PTG)-Induced Enteropathy

et al. 2018

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Celiac disease (CD) is a chronic immune-mediated disorder, characterized by enhanced paracellular permeability across the intestinal epithelium. The complex system of intercellular junctions, including tight junctions (TJs) and adherens junctions (AJs), seals together the epithelial cells to form a continuous layer. The improvements in barrier integrity have been related to modifications in intercellular junction protein expression. Polyamines (spermidine, spermine, and putrescine) actively participate in the modulation of the AJ expression. Both in vitro and in vivo studies have demonstrated that also probiotics can promote the integrity and the function of the intestinal barrier. On these bases, the present work investigated the protective effects exerted by Lactobacillus rhamnosus GG (L.GG) against the pepsin-trypsin-digested gliadin (PTG)-induced enteropathy in jejunal tissue samples of Wistar rats. In particular, the probiotic effects have been evaluated on the intestinal mucosal architecture, polyamine metabolism and intercellular junction protein expression (ZO-1, Occludin, Claudin-1, β-catenin and E-cadherin). The results from this study indicate that L.GG protects the intestinal mucosa of rats from PTG-induced damage, by preventing the reduction of the expression of the intercellular junction proteins. Consequently, a role for L.GG in the therapeutic management of the gluten-related disorders in humans could be hypothesized.

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“…14.7 kD) and one of the most commonly studied in the literature. 37 SANS provides the information on the amount of adsorption and the resultant structures formed in these system. UVÀvis spectroscopy has also been carried out to measure the adsorption isotherms prior to SANS studies.…”

Section: ' Introductionmentioning

confidence: 99%

SANS and UV–vis Spectroscopy Studies of Resultant Structure from Lysozyme Adsorption on Silica Nanoparticles

2011

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The interaction of lysozyme protein (M.W. 14.7 kD) with two sizes of silica nanoparticles (16 and 25 nm) has been examined in aqueous solution using UV-vis spectroscopy and small-angle neutron scattering (SANS). The measurements were performed on fixed concentration (1 wt %) of nanoparticles and varying concentration of protein in the range 0 to 2 wt %. The adsorption isotherm as obtained using UV-vis spectroscopy suggests strong interaction of the two components and shows an exponential behavior. The saturation values of adsorption are found to be around 90 and 270 protein molecules per particle for 16 and 25 nm sized nanoparticles, respectively. The adsorption of protein on nanoparticles leads to the aggregation of particles and these structures have been studied by SANS. The aggregates are characterized by fractal structure coexisting with unaggregated particles at low protein concentrations and free proteins at higher protein concentrations. Further, contrast variation SANS measurements have been carried out to differentiate the adsorbed and free protein in these systems.

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Enhancement of lysozyme stability and activity by polyamines

Cited by 11 publications

References 31 publications

Polyamines: Potential anti-inflammatory agents and their possible mechanism of action

Polyamines: Potential anti-inflammatory agents and their possible mechanism of action

Lactobacillus rhamnosus GG Protects the Epithelial Barrier of Wistar Rats from the Pepsin-Trypsin-Digested Gliadin (PTG)-Induced Enteropathy

SANS and UV–vis Spectroscopy Studies of Resultant Structure from Lysozyme Adsorption on Silica Nanoparticles

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