Enhancement of monoclonal antibody production in CHO cells by exposure to He–Ne laser radiation

Abstract

This study tested the effectiveness of laser biostimulation in small-scale cultures in vitro. We investigated the response of recombinant CHO cells, which are used for the production of monoclonal antibody, to low level laser radiation. The cells were irradiated using a 632.8 nm He-Ne laser in a continuous wave mode at different energy doses. We incubated the irradiated cells in small batch cultures and assessed their proliferation and productivity at various time intervals. Compared to untreated cells, the irradiated cells showed a significant increase in antibody production. Moreover, the results showed that laser irradiation did not affect viability and slightly enhanced proliferation rate.

Effects of antidiabetic drug on the anticancer activity of Cisplatin

“…About 50% of patients are still not response to Cisplatin due to drug resistance. The response of prediction patient response and development of alternative treatments is an importance in the colon cancer field [8] . Metformin could represent an alternative to conventional Cisplatin.The mode of action of Metformin in colon cancer cells has not yet understood.…”