Enhancement of Motility in Fresh and Cryo-Preserved Semen by Kallikrein and Caffeine

Kaden, R; Grossgebauer, K

doi:10.1007/978-94-009-7467-8_9

Cited by 3 publications

(4 citation statements)

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“…However, this reduction was maintained at the same level achieved with ECM. This result was to be expected, based on experience with a similar cryoprotectant [12] and the value of kallikrein in the enhancement of motility in cryopreserved semen [6]. The trend of near equality of both cryoprotectants, ECM and ICP.…”

Section: Discussionsupporting

confidence: 66%

A New Instant Cryoprotectant for Human Sperm

Kaden¹,

Klippel

Katzorke³

et al. 1985

Archives of Andrology

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A new instant cryoprotectant, mainly containing culture medium, human serum albumin, and kallikrein, and its laboratory handling in the cryoprotection of semen are described. A comparative study distributed to three cryobiological centers using either egg yolk citrate medium or instant cryoprotectant showed that neither had a significantly better or worse mean percentage recovery rate. The advantage of the new instant cryoprotectant presented consists clinically in the absence of egg yolk and technically in its easy handling. Therefore, ICP is judged as an alternative to ECM in the cryopreservation of human sperm.

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Section: Discussionsupporting

confidence: 66%

A New Instant Cryoprotectant for Human Sperm

Kaden¹,

Klippel

Katzorke³

et al. 1985

Archives of Andrology

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“…This fact has been documented for rats (Wyker and Howards -1977), for guinea pigs (Frenkel et al -1973), for bulls (Hoskins et al -1975), and for men (Mooney et al, -1972). A motility-enhancing effect is ascribed to both kallikrein and caffeine, even though the results differ as to the degree and duration of the stimulation (Bratanov et al -1978;Kaden and Grossgebauer -1980;Schill -1975;Schoenfeld -1975).…”

Section: Discussionmentioning

confidence: 89%

“…A stimulation of "spermatocele spermatozoa" by caffeine should be discussed in connection with artificial insemination, as was also mentioned by Johnson et al (1974). In addition to caffeine, the most often recommended stimulants for homologous insemination are seminal plasma (Lindholmer -1974) and kallikrein (Bratanov et al -1978;Kaden and Grossgebauer -1980;Schill -1978). To date, however, there is no proof that the "fertility" of spermatozoa is increased by such improvements in spermatozoa motility.…”

Section: Discussionmentioning

confidence: 99%

How to Increase the Motility of Spermatozoa from the Epididymis of Bulls and Alloplastic Spermatoceles in Minipigs

Hamm

Kelâmı

2009

Andrologia

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The motility of spermatozoa from the head and tail of the epididymis in bulls was studied. Qualitatively and quantitatively, the motility of spermatozoa from the cauda was distinctly better than that from the caput. It was possible to achieve a highly significant increase in the motility of epididymal spermatozoa from the caput as well as the cauda area using caffeine or a caffeinekdikrein mixture. Above all, motility stimulants improved the local motility of the epididymal spermatozoa as compared to twitching and progressive motility. The motility of caudal spermatozoa was increased by lo%, corresponding to local movement of 59% of the total number of sperm cells. It was possible to demonstrate an increase in the almost totally absent motility of the caput spermatozoa to 27% local motility. Application of kallikrein without addition of kininogens led to no significant change in spermatozoa motility.By the addition of caffeine, it was possible to increase the motility of minipig epididymal spermatozoa taken by puncture from alloplastic spermatoceles significantly. In 23 aspirates, a prompt increase in the percentage of locally motile "spermatocele spermatozoa" from 12% to 23.5% was observed. Motilitatssteigerung der Spermatozoen aus dem Nebenhoden des Bullen und aus der alloplastischen Spermatozele des MinischweinesZusammenfassung: Die Motilitat der Spermatozoen aus Nebenhodenkopf und Nebenhodenschwanz des Bullen wurde untersucht. Die Beweglichkeit der Caudaspermatozoen war qualitativ und quantitativ deutlich hoher als die der Caputspermatozoen. Durch Coffein sowie durch ein Coffein-Kallikrein-Gemisch konnte die Motilitat der Nebenhodenspermatozoen fir den Kopfbereich sowie fur den Schwanzbereich hochsignifht gesteigert werden. Vor allem die Ortsbewegung der Nebenhodenspermatozoen wurden im Vergleich zur Zitter-und Progressivbewegung durch motilitatsstimulierende Substanzen verbessert. Fur Caudaspermatozoen konnte eine Motilitatssteigerung von 100% erzielt werden, dies entsprach 59% Ortsbewegung der Gesamtspermatozoenzahl. Eine Steigerung der nahezu fehlenden Motilitat von Caputspermatozoen auf 27% Ortsbewegung liefl sich nachweisen. Kallikrein ohne zusiitzliche Gabe von Kininogen f~r t e zu keiner signifkanten Veriinderung der Spermienmotilitat.andrologia 16, (1984) Motility of Spermatozoa 21 Nebenhodenkopfspermien des Miniaturebers, die durch Punktion alloplastischer Spermatocelen gewonnen wurden, konnten durch Zugabe von Coffein signifikant verbessert werden. Eine prompte Steigerung des Anteils ortsbeweglicher ,,Spermatocele-Spermien" von 12% auf 23,5% lies sich bei der Untersuchung von 23 Aspiraten nachweisen.

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“…In the last decades, a number of drugs have been presented in the literature as having a significant positive effect on sperm motility both in vitro and in vivo. Much research has been done with kallikrein [15,18,211, proving a significant effect of this compound on sperm motility in vitro [6][7][8]161 as well as a clinical benefit [4, 11, 14, 17, 20, 221, yet only few randomized studies have been performed to compare the systemic drug effect in vivo [lo, 161. Most of these publications came from Europe and some from Japan, with criticism mainly issuing from the United States. Nevertheless, a more effective drug to improve sperm motility is currently not available.…”

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confidence: 99%

Penetration Capacity of Spermatozoa from Patients with Oligoastheno-Teratozoospermia into Bovine Cervical Mucus Before and After Therapy with Kallikrein

Maier

Eisenmenger

1994

Archives of Andrology

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Due to the high degree of subjectivity involved in quantifying sperm motility by light microscopy, it appeared of interest to compare the results obtained with a bovine mucus penetration test (Penetrak) to those of routine laboratory measurement before and after therapy with kallikrein (600 U/day). The study included 45 patients with oligoasthenoteratozoospermia (OAT syndrome) in the absence of genitourinary pathology or abnormal serum hormone levels. Infertile partnership has lasted for a mean of 3.2 years. Analysis of spermiograms and the penetration test were performed before and after 3 months of treatment. Neither of these techniques revealed significant alteration after treatment, nor was there a demonstrable success during the observation period in terms of pregnancy. Judging from these results, (1) kallikrein has no effect in patients with OAT syndrome and (2) the penetration test provides no additional information on the biological quality of ejaculate.

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Enhancement of Motility in Fresh and Cryo-Preserved Semen by Kallikrein and Caffeine

Cited by 3 publications

References 1 publication

A New Instant Cryoprotectant for Human Sperm

A New Instant Cryoprotectant for Human Sperm

How to Increase the Motility of Spermatozoa from the Epididymis of Bulls and Alloplastic Spermatoceles in Minipigs

Penetration Capacity of Spermatozoa from Patients with Oligoastheno-Teratozoospermia into Bovine Cervical Mucus Before and After Therapy with Kallikrein

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