Enhancement of native fluorescence intensity of berberine by (2-hydroxypropyl)-β-cyclodextrin in capillary electrophoresis coupled by laser-induced fluorescence detection: Application to quality control of medicinal plants

Uzaşçı, Sesil; Erim, F. Bedia

doi:10.1016/j.chroma.2014.02.068

Cited by 33 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has recently been reported that the lead compound BBR possesses fluorescence properties [22] as we confirmed in this study. Our new derivatives also possess fluorescence as proved by emission absorption spectra, and once entered the cell, remained localized only in the cytoplasm and did not reach the nucleus.…”

Section: Discussionsupporting

confidence: 77%

Effect of new berberine derivatives on colon cancer cells

Ortiz¹,

Croce²,

Aredia³

et al. 2015

ABBS

View full text Add to dashboard Cite

The natural alkaloid berberine has been recently described as a promising anticancer drug. In order to improve its efficacy and bioavailability, several derivatives have been designed and synthesized and found to be even more potent than the lead compound. Among the series of berberine derivatives we have produced, five compounds were identified to be able to heavily affect the proliferation of human HCT116 and SW613-B3 colon carcinoma cell lines. Remarkably, these active compounds exhibit high fluorescence emission property and ability to induce autophagy.

show abstract

Section: Discussionsupporting

confidence: 77%

Effect of new berberine derivatives on colon cancer cells

Ortiz¹,

Croce²,

Aredia³

et al. 2015

ABBS

View full text Add to dashboard Cite

show abstract

“…Green fluorescence originates from BBR, according to literature. 34 In short, the Nnps isolated from Coptidis Rhizoma extract were mainly formed by denaturation of a protein and can firmly adsorb BBR.…”

Section: Characterization Of the Nnpsmentioning

confidence: 99%

Natural Nano-Drug Delivery System in Coptidis Rhizoma Extract with Modified Berberine Hydrochloride Pharmacokinetics

Zhao¹,

Zhao²,

Lu³

et al. 2021

IJN

View full text Add to dashboard Cite

Purpose This study aimed to evaluate the pharmaceutical and pharmacokinetic effects of the natural nanoparticles (Nnps) isolated from Coptidis Rhizoma extract on berberine hydrochloride (BBR) and systematically explore the related mechanisms. Methods Firstly, Nnps were isolated from Coptidis Rhizoma extract and then an Nnps-BBR complex was prepared. After qualitative and quantitative analysis in terms of size, Zeta potential, morphology, and composition of the Nnps and the Nnps-BBR complex, the effects of the Nnps on the crystallization of BBR were characterized. The effects of the Nnps on the solubility and dissolution of BBR were then evaluated. In addition, the effects of the Nnps on BBR in terms of cellular uptake, transmembrane transport, metabolic stability, and pharmacokinetics in mice were studied. Results The Nnps had an average size of 166.6 ± 1.3 nm and Zeta potential of −12.5 ± 0.2 mV. The Nnps were formed by denaturation of co-existing plant proteins with molecular weight < 30 kDa. The Nnps adsorbed or dispersed BBR, thereby promoting BBR transformation from crystal to amorphous form and improving its solubility and dissolution. The Nnps carried and promoted BBR uptake by human colonic adenocarcinoma (Caco-2) cells via caveolae-mediated endocytosis, reducing P-gp-mediated efflux of BBR in mice gut sacs and Madin-Darby canine kidney cells stably expressing the transporter P-gp (MDCK-MDR1) cells. Moreover, the Nnps improved BBR metabolic stability in mouse intestinal S9, promoting BBR intestinal absorption in mice, as shown by increased peak BBR concentration (C max , 1182.3 vs 310.2 ng/mL) and exposure level (AUC 0–12 h , 2842.8 vs 1447.0 ng·h/mL) in mouse portal vein. In addition, the Nnps increased BBR exposure level in mouse livers (95,443.2 vs 43,586.2 ng·h/g liver). Conclusion The proteinaceous nanoparticles isolated from Coptidis Rhizoma extract can form a natural nano-drug delivery system with BBR, thereby significantly improving the pharmacokinetics of oral BBR.

show abstract

“…Fluorescence spectroscopy could be considered as a suitable alternative to UV-Visible spectroscopy due to its lower detection limit. Moreover, the fluorescence intensity enhancement upon CD encapsulation results in lowering the limits of detection of poorly fluorescent guests [ 86 , 87 ].…”

Section: Characterization Of Cyclodextrin Inclusion Complexesmentioning

confidence: 99%

Characterization of Cyclodextrin/Volatile Inclusion Complexes: A Review

2018

View full text Add to dashboard Cite

Cyclodextrins (CDs) are a family of cyclic oligosaccharides that constitute one of the most widely used molecular hosts in supramolecular chemistry. Encapsulation in the hydrophobic cavity of CDs positively affects the physical and chemical characteristics of the guests upon the formation of inclusion complexes. Such a property is interestingly employed to retain volatile guests and reduce their volatility. Within this scope, the starting crucial point for a suitable and careful characterization of an inclusion complex is to assess the value of the formation constant (Kf), also called stability or binding constant. This task requires the application of the appropriate analytical method and technique. Thus, the aim of the present paper is to give a general overview of the main analytical tools used for the determination of Kf values for CD/volatile inclusion complexes. This review emphasizes on the advantages, inconvenients and limits of each applied method. A special attention is also dedicated to the improvement of the current methods and to the development of new techniques. Further, the applicability of each technique is illustrated by a summary of data obtained from the literature.

show abstract

Enhancement of native fluorescence intensity of berberine by (2-hydroxypropyl)-β-cyclodextrin in capillary electrophoresis coupled by laser-induced fluorescence detection: Application to quality control of medicinal plants

Cited by 33 publications

References 14 publications

Effect of new berberine derivatives on colon cancer cells

Effect of new berberine derivatives on colon cancer cells

Natural Nano-Drug Delivery System in Coptidis Rhizoma Extract with Modified Berberine Hydrochloride Pharmacokinetics

Characterization of Cyclodextrin/Volatile Inclusion Complexes: A Review

Contact Info

Product

Resources

About