Enhancement of neutral phosphatase activity in the cytosol and nuclei of regenerating rat liver: Role of endogenous regucalcin

Omura, Masami; Yamaguchi, Masayoshi

doi:10.1002/(sici)1097-4644(19990601)73:3<332::aid-jcb5>3.3.co;2-l

Cited by 4 publications

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“…Anti‐regucalcin antibody was a monoclonal antibody raised against regucalcin prepared using standard methods [Omura and Yamaguchi, 1999]. Mice (BALB/C, Japan SLC) were subcutaneously injected with 0.1 mg/animal of antigen (rat liver regucalcin) emulsified with Freund's complete adjuvant, and 19 days later antigen (0.25 mg/animal) was intraperitoneally injected with Freund's incomplete adjuvant.…”

Section: Methodsmentioning

confidence: 99%

“…It has been shown that regucalcin has an inhibitory effect on Ca 2+ ‐dependent protein kinases [Katsumata and Yamaguchi, 1998; Yamaguchi and Katsumata, 1999] and protein phosphatase [Omura and Yamaguchi, 1998, 1999] in rat liver cells, which regucalcin is predominantly expressed [Shimokawa and Yamaguchi, 1992; Yamaguchi and Isogai, 1993]. Also, regucalcin has been demonstrated to have an inhibitory effect on protein kinases and protein phosphatases in the kidney cortex and brain of rats [Kurota and Yamaguchi, 1998; Hamamo and Yamaguchi, 2001; Morooka and Yamaguchi, 2002].…”

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confidence: 99%

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Suppressive effect of endogenous regucalcin on nitric oxide synthase activity in cloned rat hepatoma H4‐II‐E cells overexpressing regucalcin

Izumi

Tsurusaki

Yamaguchi

2003

J of Cellular Biochemistry

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The role of endogenous regucalcin, which is a regulatory protein in calcium signaling, in the regulation of nitric oxide (NO) synthase activity in the cloned rat hepatoma H4-II-E cells was investigated. Hepatoma cells were cultured for 24-72 h in the presence of fetal bovine serum (FBS; 10%). NO synthase activity in the 5,500 g supernatant of cell homogenate was significantly increased by the addition of calcium chloride (10 microM) and calmodulin (2.5 microg/ml) in the enzyme reaction mixture. The presence of trifluoperazine (TFP; 50 microM), an antagonist of calmodulin, inhibited the effect of calcium (10 microM) addition in increasing NO synthase activity, indicating the existence of Ca(2+)/calmodulin-dependent NO synthase in hepatoma cells. NO synthase activity was significantly decreased by the addition of regucalcin (10(-8) or 10(-7) M) in the reaction mixture without or with Ca(2+)/calmodulin addition. The effect of regucalcin (10(-7) M) in decreasing NO synthase activity was also seen in the presence of TFP (50 microM) or EGTA (1 mM). The presence of anti-regucalcin monoclonal antibody (10-50 ng/ml) in the reaction mixture caused a significant elevation of NO synthase activity. NO synthase activity was significantly suppressed in the hepatoma cells (transfectants) overexpressing regucalcin. This decrease was completely abolished in the presence of anti-regucalcin monoclonal antibody (50 ng/ml) in the reaction mixture. Moreover, the effect of Ca(2+)/calmodulin addition in increasing NO synthase activity in the hepatoma cells (wild-type) was completely prevented in transfectants. The present study demonstrates that endogenous regucalcin has a suppressive effect on NO synthase activity in the cloned rat hepatoma H4-II-E cells.

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Section: Methodsmentioning

confidence: 99%

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Suppressive effect of endogenous regucalcin on nitric oxide synthase activity in cloned rat hepatoma H4‐II‐E cells overexpressing regucalcin

Izumi

Tsurusaki

Yamaguchi

2003

J of Cellular Biochemistry

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“…Regucalcin, which is largely expressed in liver [Shimokawa and Yamaguchi, 1992; Yamaguchi and Isogai, 1993], has been demonstrated to translocate to the nucleus of rat liver [Tsurusaki et al, 2000]. Regucalcin has been shown to have an inhibitory effect on protein kinase and protein phosphatase activities in the nucleus of normal and regenerating rat liver which is modulated through signal transduction for hormonal stimulation [Katsumata and Yamaguchi, 1998; Omura and Yamaguchi, 1999]. Endogenous regucalcin, moreover, has been shown to have an inhibitory effect on DNA and RNA synthesis in the nucleus of normal and regenerating rat liver [Tsurusaki and Yamaguchi, 2002a,b].…”

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Overexpression of regucalcin modulates tumor‐related gene expression in cloned rat hepatoma H4‐II‐E cells

Tsurusaki

Yamaguchi

2003

J of Cellular Biochemistry

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Regucalcin is a regulatory protein in intracellular signaling pathway which is related to various protein kinases and protein phosphatases in many cells. The effect of regucalcin on the expression of tumor-related genes was investigated in the cloned rat hepatoma H4-II-E cells and the hepatoma cells (transfectants) overexpressing regucalcin. Hepatoma cells were cultured for 24-72 h in the presence of fetal bovine serum (FBS; 10%). The proliferation of hepatoma cells was significantly suppressed at 24-72 h of culture in regucalcin transfectants as compared with that of wild-type or mock-type cells. Western blot analysis showed that regucalcin was markedly expressed in transfectants. The expression of c-myc, c-fos, c-jun, Ha-ras, and p53 mRNAs was determined using reverse transcription-polymerase chain reaction (RT-PCR). Of these genes, the expression of c-myc or Ha-ras mRNAs was significantly suppressed in regucalcin transfectants. The suppression of c-myc mRNA expression in transfectants was confirmed by using Northern blot analysis; significant suppression was seen at 24, 48, or 72 h of culture in the presence of 10% FBS. Culture with 10% FBS significantly enhanced c-myc mRNA expression in the hepatoma cells (wild-type) as compared with that of 1% FBS. The enhancement was significantly abolished in the transfectants. Meanwhile, the expression of p53 mRNA in the hepatoma cells was significantly enhanced in regucalcin-overexpressing hepatoma cells. This study demonstrates that the expression of oncogene c-myc and Ha-ras mRNA in hepatoma cells overexpressing regucalcin is suppressed, and that the tumor suppression gene p53 is enhanced in the transfectants.

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“…Anti‐regucalcin antibody was a monoclonal antibody raised against regucalcin prepared using standard methods [Omura and Yamaguchi, 1999]. Mice (BALB/C, Japan SLC) were subcutaneously injected with rat liver regucalcin emulsified with Freund's complete adjuvant, and 19 days later antigen (0.25 mg/animal) was intraperitoneally injected with Freund's incomplete adjuvant.…”

Section: Methodsmentioning

confidence: 99%

A two-stage market model for microgrid power transactions via aggregators

Kim¹,

Thottan²

2011

Bell Labs Tech. J.

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In this paper, we propose a market model where microgrids sell their surplus power to a utility via aggregators. This is a scalable model where a utility does not directly interact with a large number of microgrids. Thus, aggregators collect power from microgrids and resell it to the utility. From the microgrids' perspective, aggregators are buyers. From the utility's perspective, aggregators are sellers. In this context, based on the two-stage Stackelberg game, we show how to achieve efficient market equilibrium using the tatonnement process and supply function bidding. We also show that the participation of aggregators may significantly affect the market depending on the supply elasticity of microgrids, which in turn depends on the cost structure of microgrids. For example, when the cost function of microgrids is roughly linear, the aggregators may not make a profit. However, if the cost function of microgrids has a higher order term, aggregators may accumulate a large profit, which potentially raises the issue of the regulator's role in the market. © 2011 Alcatel-Lucent.islanding operation upon a power disturbance in the electrical distribution system; however, in a normal mode of operation, microgrids are connected to the grid and can import/export power from/to the power grid [2]. In this paper we consider a market model where microgrids have surplus power and thus incentive to participate in the power market. Our goal is to capture the market behavior when there are a large number of microgrids, each with small generation capacity. Specifically, the direct participation of individual microgrids in the retail market may not be scalable since a utility faces the problem of dealing with a large number of small power sources [2]. To address this scalability problem, we introduce the concept of Korea Telecom Network Laboratory for five years prior to his tenure at Alcatel-Lucent, where he participated in Full Service Access Network (FSAN) and ITU-T SG16 FS-VDSL standardization efforts. His research interests include smart grid, demand response, information middleware, network resource allocation, optimization, network economics, and cross layer design of wireless communication systems. Dr. Kim has authored more than 30 papers in IEEE and ACM journals. MARINA THOTTAN is director of the Mission-Critical Communications and Networking Group at Alcatel-Lucent Bell Labs in Murray Hill, New Jersey, USA. She has contributed to a wide variety of projects in diverse subject areas including online gaming, content distribution, Voice over IP (VoIP), routing protocols, data over optical networks, high speed router design, network management and anomaly detection. Most recently she has been leading work on smart grid communication networks. She holds a Ph.D. degree in electrical and computer engineering from Rensselaer Polytechnic Institute, Troy, New York. Dr. Thottan has published over 40 papers in scientific journals, book chapters, and refereed conferences. She is a member of both the IEEE and ACM. ◆

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Enhancement of neutral phosphatase activity in the cytosol and nuclei of regenerating rat liver: Role of endogenous regucalcin

Cited by 4 publications

References 0 publications

Suppressive effect of endogenous regucalcin on nitric oxide synthase activity in cloned rat hepatoma H4‐II‐E cells overexpressing regucalcin

Suppressive effect of endogenous regucalcin on nitric oxide synthase activity in cloned rat hepatoma H4‐II‐E cells overexpressing regucalcin

Overexpression of regucalcin modulates tumor‐related gene expression in cloned rat hepatoma H4‐II‐E cells

A two-stage market model for microgrid power transactions via aggregators

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