Enhancement of NO release from S-nitrosoalbumin by pollution derived metal ions

Wądołek, Anna; Oszajca, Maria; Pęcak, Wiktoria; Brindell, Małgorzata; Stochel, Grażyna

doi:10.1039/d1dt01260f

Cited by 4 publications

(10 citation statements)

References 35 publications

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“…An intriguing parameter requiring analysis was the time delay between the GSNO injection and the actual signal growth registered for NO signal development. In our previous model studies, an immediate increase in the current signal was registered after S-nitrosothiols injection to the studied reaction solution [40,41]. A significant difference in the delay time was observed for the plasma samples from CVD subjects vs. healthy control (p = 0.004 and p < 0.001 for CHFE and CAD, respectively).…”

Section: Influence Of Urban Pm Extracts On the Gsno Decomposition In The Blood Plasma Environmentmentioning

confidence: 86%

“…After this time, suspensions were filtered through molecular weight cut-off membrane-based centrifugal filters (Amicon Ultra-4, MWCO 100 kDa). The metal content of the SRM extract solution was recently reported by us [40] and was also given in the Supplementary Materials.…”

Section: Particulate Matter's Extract Preparationmentioning

confidence: 96%

“…It was previously shown that the reduced form of the copper ion (Cu + ) is mainly responsible for S-nitrosothiols decomposition in the presence of SRM extract pretreated with AscA [40,41,50]. The observed strongly elevated and accelerated release of NO from GSNO in plasma environment from CVD participants compared to the control may be due to the ineffective binding the SRM extract derived transition metal ions, in particular redox-active copper ions.…”

Section: Effect Of Ascorbic Acid On the Gsno Decomposition By Urban Pm Extracts In The Blood Plasma Environmentmentioning

confidence: 99%

“…The oxidized thiol group can be converted to sulfenic acid, then to disulfide with the potential to be redox cycled to its primary form (HSA-SH) in the reaction with other free thiols [57,58]. The thiol moiety is also highly reactive and capable to undergo nitrosylation (HSA-NO) resulting in the formation of S-nitrosoalbumin, which is more resistant against degradation by SRM extract than GSNO [37,40]. Furthermore, hydrogen peroxide or hydroxyl radical, potentially formed in the process of ascorbic acid oxidation catalyzed by copper ions delivered from SRM extracts, might be trapped by Cys34 of albumin [59].…”

Section: Free Thiol Content In Human Plasma and Its Influence On No Release From Gsnomentioning

confidence: 99%

“…Since Cys34 can readily respond to transition metal ions (either by binding or oxidation), [39] its function can be disturbed by the exposure of the plasma to pollutants. Furthermore, the negative influence of the PM-derived transition metal ions on the stability of S-nitrosoalbumin (HSA-NO) and low-molecular-weight S-nitrosothiols was also demonstrated [40,41]. Moreover, metal ions originating from the inhaled PM, after encountering the lung lining fluid containing a substantial concentration of ascorbic acid, may change their oxidation state and potency to decompose S-nitrosothiols.…”

Section: Introductionmentioning

confidence: 99%

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Blood Plasma’s Protective Ability against the Degradation of S-Nitrosoglutathione under the Influence of Air-Pollution-Derived Metal Ions in Patients with Exacerbation of Heart Failure and Coronary Artery Disease

Wądołek

Drwiła

Oszajca

et al. 2021

IJMS

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One of the consequences of long-term exposure to air pollutants is increased mortality and deterioration of life parameters, especially among people diagnosed with cardiovascular diseases (CVD) or impaired respiratory system. Aqueous soluble inorganic components of airborne particulate matter containing redox-active transition metal ions affect the stability of S-nitrosothiols and disrupt the balance in the homeostasis of nitric oxide. Blood plasma’s protective ability against the decomposition of S-nitrosoglutathione (GSNO) under the influence of aqueous PM extract among patients with exacerbation of heart failure and coronary artery disease was studied and compared with a group of healthy volunteers. In the environment of CVD patients’ plasma, NO release from GSNO was facilitated compared to the plasma of healthy controls, and the addition of ascorbic acid boosted this process. Model studies with albumin revealed that the amount of free thiol groups is one of the crucial factors in GSNO decomposition. The correlation between the concentration of NO released and -SH level in blood plasma supports this conclusion. Complementary studies on gamma-glutamyltranspeptidase activity and ICP-MS multielement analysis of CVD patients’ plasma samples in comparison to a healthy control group provide broader insights into the mechanism of cardiovascular risk development induced by air pollution.

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Section: Influence Of Urban Pm Extracts On the Gsno Decomposition In The Blood Plasma Environmentmentioning

confidence: 86%

Section: Particulate Matter's Extract Preparationmentioning

confidence: 96%

Section: Effect Of Ascorbic Acid On the Gsno Decomposition By Urban Pm Extracts In The Blood Plasma Environmentmentioning

confidence: 99%

Section: Free Thiol Content In Human Plasma and Its Influence On No Release From Gsnomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Blood Plasma’s Protective Ability against the Degradation of S-Nitrosoglutathione under the Influence of Air-Pollution-Derived Metal Ions in Patients with Exacerbation of Heart Failure and Coronary Artery Disease

Wądołek

Drwiła

Oszajca

et al. 2021

IJMS

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Triphenylmethyl Thionitrite: An Efficient NO Transfer Reagent During the Synthesis of a Triruthenium Nitrosyl Cluster

Heinemann

Böttcher

2022

Zeitschrift anorg allge chemie

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The synthesis and structural characterization of the novel triruthenium nitrosyl cluster [Ru3(μ‐NO)(CO)5(μ‐H)(μ‐PBut2)2(μ‐dppm)] (2) is described. Compound 2 was obtained by the reaction of the 46‐valence electron cluster [Ru3(μ‐CO)(CO)4(μ3‐H)(μ‐H)(μ‐PBut2)2(μ‐dppm)] (1) with nitric oxide and on the other hand in a more efficient way by the reaction of 1 with the nitrosyl transfer reagent triphenylmethyl thionitrite (TTN). The reaction of 1 with NO was outlined in dichloromethane whereas the reaction with TTN was realized in THF. Both methods worked at ambient temperature affording compound 2 in high yield. Temporarily we found that the transformation of 1 with NO was accompanied by a fast side‐reaction resulting in a further substitution of CO ligands. The molecular structure of 2 in the crystal was confirmed by single‐crystal X‐ray diffraction.

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Investigation of the Immunogenic Properties of Ovalbumin Modified by Urban Airborne Particulate Matter

Nowak

Wądołek

Mazuryk

et al. 2023

Arch. Immunol. Ther. Exp.

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Exposure to air particulate matter (PM) is linked to the blood oxidative stress and systemic inflammation. The aim of this study was to elucidate whether oxidative PM modification of ovalbumin (OVA), the major antioxidant serum protein, may alter its antigenicity and/or immunogenicity. Ovalbumin was exposed via dialysis to the standard urban PM (SRM 1648a) or to PM with removed organic content (encoded as LAP). Both structural changes and biological properties of PM-modified OVA were measured. T lymphocytes and dendritic cells (the major antigen-presenting cells) isolated from C57BL/6 and OT-II (323–339 epitope) OVA-specific T cell receptor (TCR)-transgenic mice were used to test the effect of PM on OVA immunogenicity. The immunogenicity of both SRM 1648a and LAP-modified OVA was significantly higher than that of control OVA, as measured by the epitope-specific T cell proliferation and interferon γ production by the stimulated cells. This effect was associated with mild oxidative changes in the carrier molecule outside the structure of the OVA epitope and with increased resistance to proteolysis of PM-modified OVA. Interestingly, dendritic cells showed enhanced capacity for the uptake of proteins when the cells were cultured with PM-modified OVA. Our results suggest that the enhanced immunogenicity of PM-modified OVA is not associated with altered antigenicity or antigen presentation. However, it may result from slower degradation and longer persistence of modified antigens in dendritic cells. Whether this phenomenon is associated with enhanced risk prevalence of autoimmune diseases observed in the areas with high urban PM pollution needs to be explained.

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Enhancement of NO release from S-nitrosoalbumin by pollution derived metal ions

Abstract: S-Nitrosoalbumin stability is affected by pollution derived metal ions. An inorganic aqueous soluble urban PM fraction might disturb the S-nitrosylation/denitrosylation balance.

Cited by 4 publications

References 35 publications

Blood Plasma’s Protective Ability against the Degradation of S-Nitrosoglutathione under the Influence of Air-Pollution-Derived Metal Ions in Patients with Exacerbation of Heart Failure and Coronary Artery Disease

Blood Plasma’s Protective Ability against the Degradation of S-Nitrosoglutathione under the Influence of Air-Pollution-Derived Metal Ions in Patients with Exacerbation of Heart Failure and Coronary Artery Disease

Triphenylmethyl Thionitrite: An Efficient NO Transfer Reagent During the Synthesis of a Triruthenium Nitrosyl Cluster

Investigation of the Immunogenic Properties of Ovalbumin Modified by Urban Airborne Particulate Matter

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