Enhancement of Ocular Drug Penetration

Sasaki, Hidetada; Yamamura, Kenzo; Mukai, Takahiro; Nishida, Koyo; Nakamura, Junzo; Nakashima, Makoto; Ichikawa, Masataka

doi:10.1615/critrevtherdrugcarriersyst.v16.i1.20

Cited by 123 publications

(65 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…31,32 Corneal drug permeability has been evaluated by diffusion experiments in vitro. 9 The epithelial barrier function in humans has been examined by measuring the permeability of fluorescence. [33][34][35] Drug toxicity must be rapidly evaluated because topically instilled drugs become rapidly diluted with tears.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these adjuvants act as ocular penetrating enhancers, promoting drug penetration through the strong corneal barrier and modifying the physiochemical property of drugs. 8,9 At the same time, however, they damage the corneal epithelial structure, especially the transcellular integration of superficial cells, which is mainly maintained by tight junctions. 10,11 Therefore, investigation of the effect on the cornea of ophthalmic drugs and adjuvants is important.…”

Section: Introduction Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Less Invasive Corneal Transepithelial Electrical Resistance Measurement Method

et al. 2016

Self Cite

View full text Add to dashboard Cite

Purpose: To evaluate acute corneal permeability changes after instillation of benzalkonium chloride (BAC) using a newly developed in vivo less invasive corneal TER measurement method in animals and humans. Methods: We previously developed an in vivo method for measuring corneal transepithelial electrical resistance (TER) using intraocular electrodes in animals. This method can be used to precisely measure the decline of the corneal barrier function after instillation of BAC. To lessen the invasiveness of that procedure, we further refined the method for measuring the corneal TER by developing electrodes that could be placed on the surface of the cornea and in the conjunctival sac instead of inserting them into the anterior chamber. Corneal TER changes before and after exposure to 0.02% BAC were determined in this study using the new device in both rabbits and humans. Results: There was a significant decrease in the corneal TER after exposure of the cornea to 0.02% BAC solution in both rabbits and humans (P.01). The results of this new less invasive method agreed with those of formerly established anterior chamber methods as regards rabbits experiment and was expected and satisfactory as regards human experiment.Conclusion: This new less invasive corneal TER measurement method enables us for the first time to measure TER of the human cornea, allowing safe and reliable investigation of the direct effect of different eye drops treatments on the corneal epithelium.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introduction Introductionmentioning

confidence: 99%

Less Invasive Corneal Transepithelial Electrical Resistance Measurement Method

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…They are often essential for producing ocular liquid formulation, which solubilize drugs and prevent microbial contamination. Some of these adjuvants act as ocular penetrating enhancers promoting drug penetration through the strong corneal barrier and modifying the physiochemical property of drugs (Saettone et al, 1996: Sasaki et al, 1999. At the same time, however, they destroy the corneal epithelial structure, especially the transcellular integration of superficial cells, which is mainly maintained by tight junctions (Burstein, 1980: Tonjum, 1975.…”

Section: Discussionmentioning

confidence: 99%

“…Alternative methods include evaluation of toxicity in cultured ocular cells (Kruszewski et al, 1997), direct confocal microscopic analysis (Furrer et al, 2000), and various other approaches using isolated animal corneas (Monti et al, 2002: Prinsen, 1996. Corneal drug permeability has been evaluated by diffusion experiment in vitro (Sasaki et al, 1999). The epithelial barrier function in humans has been examined by measuring the permeability of fluorescence (Gobbels and Spitznas, 1989: Ishibashi et al, 2003: Joshi et al, 1996.…”

Section: Discussionmentioning

confidence: 99%

A novel in vivo corneal trans-epithelial electrical resistance measurement device

Uematsu

Mohamed

Onizuka

et al. 2015

Journal of Pharmacological and Toxicological Methods

View full text Add to dashboard Cite

Purpose: To develop a device that is capable of easily measuring corneal transepithelial electrical resistance (TER) and changes in the corneal barrier function. Methods:We had previously developed an in vivo method for measuring corneal TER using intraocular electrode. This method can be used to precisely measure the decline of the corneal barrier function after instillation of benzalkonium chloride (BAC). In order to lessen the invasiveness of that procedure, we further refined the method for measuring the corneal TER by developing electrodes that could be placed on the cornea and in the conjunctival sac instead of inserting them into the anterior chamber. TER was then calculated by subtracting the electrical resistance, which lacked the corneal epithelial input, from the whole electrical resistance that was measured between the electrodes. Slit lamp examination and scanning electron microscopy (SEM) were used to determine safety of the new device. Corneal TER changes after exposure to 0.02% BAC were determined using the new device as well as SEM and transmission electron microscopy (TEM).Results: Slit lamp examination before and after exposure of rabbits' corneas to the sensor confirmed safety of the device. SEM examination revealed no difference of the corneal epithelium which exposed to the new device with normal corneas. SEM and 3 TEM pictures revealed damaged microvilli and tight junctions after instillation of 0.02% BAC. TER change after treatment with 0.02%BAC was similar to those determined by the established anterior chamber method. Conclusion:We succeeded to develop a less invasive device for corneal TER measurement in vivo in animals. This new device may be applicable in the future for clinical use in humans.

show abstract

“…7]. Many parts of the body can be addressed, including teeth [17] and eyes [25,26]. Vasodilatation is less pronounced for anodal than for cathodal drugs [27] and zwitterionic drugs such as aminolevulinic acid (ALA) are more efficiently delivered from the anode than the cathode [28].…”

mentioning

confidence: 99%

Biomedical applications of batteries

Latham

2004

Solid State Ionics

View full text Add to dashboard Cite

An overview is presented of the many ways in which batteries and battery materials are used in medicine and in biomedical studies. These include the use of batteries as power sources for motorised wheelchairs, surgical tools, cardiac pacemakers and defibrillators, dynamic prostheses, sensors and monitors for physiological parameters, neurostimulators, devices for pain relief, and iontophoretic, electroporative and related devices for drug administration. The various types of battery and fuel cell used for this wide range of applications will be considered, together with the potential harmful side effects, including accidental ingestion of batteries and the explosive nature of some of the early cardiac pacemaker battery systems. +44-116-257-7146 0r +44 -7703-21185;, fax +44-116-250-6431; email rgl@dmu.ac.uk AbstractAn overview is presented of the many ways in which batteries and battery materials are used in medicine and in biomedical studies. These include the use of batteries as power sources for motorised wheelchairs; surgical tools; cardiac pacemakers and defibrillators; dynamic prostheses; sensors and monitors for physiological parameters; neurostimulators; devices for pain relief; and iontophoretic, electroporative and related devices for drug administration. The various types of battery and fuel cell used for this wide range of applications will be considered, together with the potential harmful side effects, including accidental ingestion of batteries and the explosive nature of some of the early cardiac pacemaker battery systems.

show abstract

Enhancement of Ocular Drug Penetration

Cited by 123 publications

References 0 publications

Less Invasive Corneal Transepithelial Electrical Resistance Measurement Method

Less Invasive Corneal Transepithelial Electrical Resistance Measurement Method

A novel in vivo corneal trans-epithelial electrical resistance measurement device

Biomedical applications of batteries

Contact Info

Product

Resources

About