Enhancement of permeability of glycerol with ultrasoundin human normal and cancer breast tissues in vitro using optical coherence tomography

Abstract

Here we examined the effect of human normal breast (NB) and breast cancer (BC) tissues in vitro after treatment with glycerol in conjunction with ultrasound (surgeonperformed, SP) by OCT for functional imaging to monitor. 60% glycerol (G) and SP was simultaneously applied for 5 min. Depth-and time-resolved profiles for OCT signal enhancement were presented. The results show that OCT imaging depth of breast tissues after treatment with 60% G in combined with SP more obviously improved than that after application of glycerol alone. The permeability coefficient of 60% glycerol in 60% G/NB, 60% G/BC, 60% G/SP/NB, and 60% G/SP/BC were (0.91±0.02)×10 cm/s, (1.63±0.04)×10−5 cm/s, and (7.98±0.19)×10 −5 cm/s, respectively. The permeability coefficient of glycerol in 60% G/SP/NB was 1.84-fold than that in 60% G/NB, but there was 2.54-fold on permeability coefficient comparing 60% G/SP/BC with that in 60% G/BC. The results from this study indicated that the permeability coefficient of glycerol not only in normal breast tissues, but also in breast cancer tissues after treatment with ultrasound is larger than that in tissues without ultrasound. The relative permeability coefficient of 60% glycerol diffusion of normal breast and breast cancer treatment with ultrasound than without ultrasound for 5 min

Quantification of glucose and glycerol diffusion in myocardium

“…The 60%-glycerol solution permeation coe±cient was presented for breast tissues as ð0:89 AE 0:02ÞÂ 10 À5 cm/s. 67 For human skin, permeation coe±-cient of 40%-glycerol solution has been estimated as ð1:67 AE 0:04Þ Â 10 À6 cm/s. 26 The di®erence between the di®usion (permeation) coe±cients obtained in this study and the ones presented by the other authors is explained mostly by the di®erences of structural properties of the investigated tissues.…”

Real-time display with large field of view on fourier domain optical coherence tomography at 1310 nm wavelength for dermatology

“…Compared with ultrasound techniques, der moscopy [1,2], confocal reflectance microscopy [3][4][5], and multiphoton excitation fluorescence micros copy [6], optical coherence tomography (OCT) is the only method for cross sectional imaging of the epider mis and upper dermis in vivo with high resolution of 15 µm both in the lateral and the axial directions [7][8][9][10][11][12][13]. At present, OCT has been used for the investiga tion of wound healing [14,15], inflammatory skin [16,17], estimating sun damage [18], and diagnosing tumors [19][20][21][22].…”

Line-scan Raman microscopy complements optical coherence tomography for tumor boundary detection

“…OCT has also been applied in the field of embryology [14,15]. OCT has also been used for monitoring changes in optical properties due to applications of chemicals such as glucose and glycerol [16,17], and this has further been extended to differentiate cancerous tissues from normal ones [18,19]. Raman spectroscopy, on the other hand, has been employed to identify intraoperative margin based on tissue variations at the molecular level [8].…”