Enhancement of retinal pigment epithelial culture characteristics and subretinal space tolerance of scaffolds with 200 nm fiber topography

Liu, Zengping; Yu, Na; Holz, Frank G.; Yang, Fang; Stanzel, Boris V.

doi:10.1016/j.biomaterials.2013.12.069

Cited by 101 publications

(86 citation statements)

References 62 publications

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“…6 In addition, these substrates should be biocompatible, able to maintain the RPE phenotype, and exhibit favorable surgical properties (handling stability and resistance to tear). 3,10 Recent works in the development of biocompatible substrates to patch or replace diseased BM for the delivery and long-term survival of RPE transplants include: polyethylene terephthalate and poly(L-lactide-co-ε-caprolactone) films and electrospun substrates 10 ; montmorillonite clay based polyurethane substrates 12 ; methacrylate-based copolymer electrospun fibrous scaffold 13 ; ultrathin and biofunctionalized polyimide membranes 7 ; and ultrathin collagen membranes. 14 Gluconacetobacter xylinus bacteria synthesize bacterial cellulose (BC) in a complex process that results in a three-dimensional gelatinous structure formed on the surface of a liquid medium.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

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Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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“…polyethylene terephthalate and poly(L-lactide-co-ε-caprolactone); parylene, already patented for stem cell therapy to treat diseased or damaged ocular tissue; montmorillonite clay-based polyurethane nanocomposite; copolymers of methyl methacrylate and poly(ethylene glycol) methacrylate; and gelatin-biofunctionalized polyimide membranes [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves

Rodrigues

Padrão

et al. 2016

Colloids and Surfaces B: Biointerfaces

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This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored. Therefore, the ABC substrates coated with UBM showed a great potential to be applied as cell carriers in RPE transplantation.

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“…Many study variables such as cell carrier variants, fetal, adult or stem cell derived RPE cell sources, choices for immunosuppressants, etc., can be explored 14,26,27,34 . Further improvement such as serum-free RPE culture methods, characterization of xenoRPE in subretinal space, removal of the host RPE layer 14 or strategies for implant anchorage are current work in progress.…”

Section: Discussionmentioning

confidence: 99%

A Step by Step Protocol for Subretinal Surgery in Rabbits

Al-Nawaiseh

Thieltges

Liu

et al. 2016

JoVE

Self Cite

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Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision.Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology.The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models.

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Enhancement of retinal pigment epithelial culture characteristics and subretinal space tolerance of scaffolds with 200 nm fiber topography

Cited by 101 publications

References 62 publications

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

A Step by Step Protocol for Subretinal Surgery in Rabbits

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