Enhancement of Skin Whitening and Anti-wrinkle Activities of the Co-culture of Lactobacillus rhamnosus and Lactobacillus paracasei

In this study, we developed Lava-Dermabiotics HDB1234 using Lactobacillus plantarum HDB1234 and lava seawater fermentation technology to enhance skin barrier. The use of lava seawater increased the purity and concentration of nucleotides in Lava-Dermabiotics HDB1234 by using nanodrop 2000 spectrophotometer. Also, when 5%(v/v) Lava-Dermabiotics HDB1234 was treated, cytotoxicity on normal human epidermal keratinocytes (NHEKs) and human fibroblast cells was not observed by using MTT assay. The mRNA expressions of claudin-1 (CLDN-1), serine palmitoyltransferase (SPT), and filaggrin (FLG) on Lava-Dermabiotics HDB1234 treated cells were dose-dependent higher than those of non-treated cells. Especially, the protein expression of FLG was also confirmed by using western blotting and immunocytochemistry staining. These results indicated that Lava-Dermabiotics HDB1234 could be used as a potential cosmetic ingredient for skin barrier enhancement through CLDN-1, SPT, and FLG expression related to the formation of skin barrier.

Skin Barrier Enhancement of Ferment using Lava Seawater and Lactobacillus plantarum HDB1234 as a Novel Cosmetic Ingredient

Yang¹,

Lee²,

Yoon³

et al. 2021

Relaxing Effect of Novel Cosmetic Ingredient using Lactobacillus gasseri HDB1102 on Skin Problems Caused by Particulate Matter

Yang¹,

Kim²,

Song³

et al. 2021

In this study, we developed Dermabiotics HDB1102 using Lactobacillus gasseri HDB1102 to relieve skin irritation caused by particulate matter (PM). L. gasseri HDB1102 was provided from cell bank and identified by 16S ribosomal RNA gene sequencing. Dermabiotics HDB1102 was manufactured by heating, centrifuging, and filtering culture medium of L. gasseri HDB1102. When 0-2.5%(v/v) Dermabiotics HDB1102 was treated, cytotoxicity on normal human epidermal keratinocytes (NHEKs) and human fibroblast was not observed by using MTT assay. The mRNA expression levels of cytochrome P450 1A1 (CYP1A1), interleukin (IL)-1β, and IL-8 on Dermabiotics HDB1102 treated cells decreased compared to PM-treated cells. Conversely, the mRNA expressions of aquaporin-3 (AQP-3), CD-44, and collagen type 1 (COL-1) on Dermabiotics HDB1102 treated cells were dose-dependent higher than those of non-treated cells. These results indicated that Dermabiotics HDB1102 have anti-inflammatory, moisturizing, and anti-wrinkle effects and could be used as a potential cosmetic ingredient to alleviate skin symptoms caused by PM.

Skin Barrier-enhancing, Antiwrinkle, and Antimelanogenic Effects of Probiotic Lysates Composed of Nucleotides

Kim¹,

Kim²,

Cha³

et al. 2021

Several previous studies have investigated the skin aging prevention effects of ceramide, hyaluronic acid, and natural or fermented plant materials. Recently, oral administration and dermal application of probiotics or probiotic lysates have shown antiaging effects. The purpose of this study is to optimize the preparation of probiotic lysates with a high concentration of nucleotides and to confirm the effects of probiotic lysates on the skin. Probiotic lysates were prepared by heating at 121°C for various periods with adding of sodium hyaluronic acid. Probiotic lysates of Bifidobacterium longum HDB7072, Lactobacillus paracasei HDB1196, and Lactobacillus acidophilus HDB1014 were applied to normal human epidermal keratinocytes (NHEKs), fibroblast cells, and B16F1 cells, respectively. Cell viability, antioxidant effects, and mRNA expression were evaluated by using MTT assays, DPPH assays, and qRT-PCR. Probiotic lysates prepared by heating the culture medium at 121°C for 2 h with 0.5% sodium hyaluronic acid showed the highest nucleotide concentration. In the three tested skin cells, the cell viability of filtered lysates was similar or higher to that of unfiltered lysates. HDB7072 lysates increased filaggrin expression in NHEKs. HDB1196 lysates showed DPPH radical-scavenging and antiwrinkle effects through the downregulation of matrix metalloproteinase-1 and upregulation of collagen type 1 in fibroblasts. HDB1014 lysates had antioxidant and antimelanogenic effects in B16F1 cells. Cell wall-removed probiotic lysates could be used as novel ingredients to improve skin aging and skin barrier issues.