Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Abstract

This work concerns the study of the enhancement of surfactin and fengycin production by B. mojavensis A21 and application of the produced product in diesel biodegradation. The influences of the culture medium and cells immobilization were studied. The highest lipopeptides production was achieved after 72 hours of incubation in a culture medium containing 30 g/L glucose as carbon source and a combination of yeast extract (1 g/L) and glutamic acid (5 g/L) as nitrogen sources with initial pH 7.0 at 30°C and 90% volumetric aeration. The study of primary metabolites production showed mainly the production of acetoin, with a maximum production after 24 h of strain growth. The use of immobilized cells seemed to be a promising method for improving lipopeptides productivity. In fact, the synthesis of both lipopeptides, mainly fengycin, was greatly enhanced by the immobilization of A21 cells. An increase of diesel degradation capacity of approximately 20, 27, and 40% in the presence of 0.5, 1, and 2 g/L of produced lipopeptides, respectively, was observed. Considering these properties, B. mojavensis A21 strain producing a lipopeptide mixture, containing both surfactin and fengycin, may be considered as a potential candidate for future use in bioremediation and crop protection.

Caracterización del potencial de degradación de compuestos xenobióticos por la rizobacteria Azospirillum brasilense

“…La liberación de biosurfactantes es una de las estrategias utilizadas por los microorganismos para influir en la absorción de hidrocarburos y compuestos hidrofóbicos en general (Johnsen et al 2005, Chong & Li, 2017; debido a que la estructura de los biosurfactantes permite solubilizar los hidrocarburos y con esto hacerlos disponibles para la degradación (Nievas et al, 2008, Hmidet et al, 2017. A pesar de obtener crecimiento de cada una de los 17 biotipos de Azospirillum brasilense en medio base sangre, ninguna de ellas presentó beta hemolisis.…”

“Screening and Optimization of Agro-industrial wastes for glycoprotein biosurfactant production from Sphingobacterium thalpophilum DP9”

“…Effect of aeration on biosurfactant was assayed by lling different volume of media in 250 ml asks and it was observed that 40 % of aeration (100 ml media in 250 ml ask) was most suitable (E24% = 47.41 ± 1.62%), which followed by 30 % (E24% = 38.00 ± 2.30%) and 20% (E24% = 27.825 ± 1.73%) ( Fig.2(a)). Bacillus mojavensis A21 produced highest surfactin when cultured in 10% ask (25 ml media in 250 ml of ask) while the production in decreased when culture was kept in 70% aeration ( 75 ml media in 250 ml ask) (Hmidet et al 2017). Bacillus subtilis BS5 produced maximum surfactin 10% media containing ask where as production is decline sharply when media volume was increased from 10% (Abdel-mawgoud et al 2008).…”

CHARACTERIZATION OF A Microbacterium sp. STRAIN ISOLATED FROM SOILS CONTAMINATED WITH HYDROCARBONS IN THE BURGOS BASIN, MEXICO

“…Biosurfactant release is a metabolic strategy used by microorganisms in the adsorption of hydrocarbons and hydrophobic compounds in general (Johnsen et al 2005, Chong andLi 2017). It facilitates hydrocarbon degradation (Hmidet et al 2017, Nievas et al 2008. Numerous bacteria, such as Pseudomonas sp.…”

“…(Liu et al 2019), and Bacillus sp. (Hmidet et al 2017) degrade xenobiotics; however, since many are potential human pathogens, they can represent a health risks, making their use complicated.…”