Enhancement of Surfactin yield by improving the medium composition and fermentation process

Willenbacher, Judit; Yeremchuk, Wladimir; Mohr, Teresa; Syldatk, Christoph; Hausmann, Rudolf

doi:10.1186/s13568-015-0145-0

Cited by 62 publications

(53 citation statements)

References 24 publications

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“…Besides that, foam resulting from emulsification of biosurfactants due to vigorous agitation during cultivation might decrease the transfer efficiency of oxygen into the liquid medium [ 17 ] in which countermeasures can be taken to eliminate foaming but will further drive-up the production cost. To overcome these problems, various researchers were looking into cheaper alternative substrates, optimization of the environmental conditions, and improvement on the downstream recovery processes [ 18 , 19 , 20 , 21 ]. Still, no definitive method could contribute to the overall cost saving for biosurfactant production.…”

Section: Introductionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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Surfactants are compounds that can reduce the surface tension between two different phases or the interfacial tension of the liquid between water and oil, possessing both hydrophilic and hydrophobic moieties. Biosurfactants have traits that have proven to be advantageous over synthetic surfactants, but these compounds do not compete economically with synthetic surfactants. Different alternatives increase the yield of biosurfactants; development of an economical production process and the usage of cheaper substrates during process have been employed. One of the solutions relies on the suitable formulation of a production medium by including alternative raw materials sourced from agro-wastes, hydrocarbons, or by-products of a process might help in boosting the biosurfactant production. Since the nutritional factors required will be different among microorganisms, the establishment of a suitable formulation for biosurfactant production will be challenging. The present review describes various nutrients and elements considered in the formulation of a production medium with an approach focusing on the macronutrient (carbon, nitrogen source, and C/N ratio), minerals, vitamins, metabolic regulators, and salinity levels which may aid in the study of biosurfactant production in the future.

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Section: Introductionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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“…Numerous studies concerning free cells production of lipopeptides have been reported. Several parameters such as carbon sources, nitrogen sources, and temperature were studied [ 11 , 12 ]. In addition, the production of biosurfactants by immobilized cells of Bacillus subtilis was well studied [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Hmidet

Ayed

Jacques

2017

BioMed Research International

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This work concerns the study of the enhancement of surfactin and fengycin production by B. mojavensis A21 and application of the produced product in diesel biodegradation. The influences of the culture medium and cells immobilization were studied. The highest lipopeptides production was achieved after 72 hours of incubation in a culture medium containing 30 g/L glucose as carbon source and a combination of yeast extract (1 g/L) and glutamic acid (5 g/L) as nitrogen sources with initial pH 7.0 at 30°C and 90% volumetric aeration. The study of primary metabolites production showed mainly the production of acetoin, with a maximum production after 24 h of strain growth. The use of immobilized cells seemed to be a promising method for improving lipopeptides productivity. In fact, the synthesis of both lipopeptides, mainly fengycin, was greatly enhanced by the immobilization of A21 cells. An increase of diesel degradation capacity of approximately 20, 27, and 40% in the presence of 0.5, 1, and 2 g/L of produced lipopeptides, respectively, was observed. Considering these properties, B. mojavensis A21 strain producing a lipopeptide mixture, containing both surfactin and fengycin, may be considered as a potential candidate for future use in bioremediation and crop protection.

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“…It was previously shown that simple cultivation integrated foam fractionation is an effective tool for biosurfactant production e.g. of surfactin using Bacillus subtilis (Chen et al 2006 ; Willenbacher et al 2014 , 2015 ) or HFBII using Saccharomyces cerevisiae (Winterburn et al 2011 ). The usability of foam fractionation in rhamnolipid purification and concentration in a cell free process was shown previously (Sarachat et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor

et al. 2016

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Heterologeous production of rhamnolipids in Pseudomonas putida is characterized by advantages of a non-pathogenic host and avoidance of the native quorum sensing regulation in Pseudomonas aeruginosa. Yet, downstream processing is a major problem in rhamnolipid production and increases in complexity at low rhamnolipid titers and when using chemical foam control. This leaves the necessity of a simple concentrating and purification method. Foam fractionation is an elegant method for in situ product removal when producing microbial surfactants. However, up to now in situ foam fractionation is nearly exclusively reported for the production of surfactin with Bacillus subtilis. So far no cultivation integrated foam fractionation process for rhamnolipid production has been reported. This is probably due to excessive bacterial foam enrichment in that system. In this article a simple integrated foam fractionation process is reported for heterologous rhamnolipid production in a bioreactor with easily manageable bacterial foam enrichments. Rhamnolipids were highly concentrated in the foam during the cultivation process with enrichment factors up to 200. The described process was evaluated at different pH, media compositions and temperatures. Foam fractionation processes were characterized by calculating procedural parameter including rhamnolipid and bacterial enrichment, rhamnolipid recovery, YX/S, YP/X, and specific as well as volumetric productivities. Comparing foam fractionation parameters of the rhamnolipid process with the surfactin process a high effectiveness of the integrated foam fractionation for rhamnolipid production was demonstrated.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0183-2) contains supplementary material, which is available to authorized users.

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Enhancement of Surfactin yield by improving the medium composition and fermentation process

Cited by 62 publications

References 24 publications

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor

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