Enhancement of the antifungal activity ofBacillus subtilisF29-3 by the chitinase encoded byBacillus circulans chiAgene

Chen, Chao‐Ying; Wang, Yi-Huei; Huang, Chang‐Jen

doi:10.1139/w04-027

Cited by 31 publications

(23 citation statements)

References 25 publications

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“…Shanmugaiah et al (2008) reported that among the 39 chitinolytic bacteria which were isolated from rice rhizosphere, only 11 isolates produced zone of clearance over 0.5 cm on chitin colloidal agar. Chitinase production was reported in different species of Bacillus such as B. amyloliquefaciens (Wang et al, 2002), B. cereus (Huang et al, 2005), B. circulans (Chen et al, 2004), B. licheniformis (Waldeck et al, 2006), B. megaterium (Donderski and Brzezinska, 2005), B. stearothermophilus (Sakai et al, 1994), B. subtilis (Wang et al, 2006), B. thuringiensis sub sp. aizawai (De la Vega et al, 2006), B. thuringiensis sub sp.…”

Section: Discussionmentioning

confidence: 99%

Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Its Application as Biocontrol for Whitefly (Bemisia tabaci Genn.)

Mubarik¹,

Mahagiani²,

Anindyaputri³

et al. 2010

American Journal of Agricultural and Biological Sciences

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Problem statement: Chitin, a common constituent of insect exoskeleton, could be hydrolyzed by chitinase. The research was conducted to screen chitinolytic rhizobacteria isolated from rhizosphere of chilli pepper and to determine their chitinase activity in degrading chitin of whitefly, Bemisia tabaci Genn. (Hemiptera: Aleyrodidae). Whitefly is recognized as an important pest on many crops including chilli pepper. Approach: Screening and molecular identification based on 16S rRNA sequence of chitinolytic isolates, chitinase productions, measurement of chitinase activity, characterization of chitinase and effect of the chitinase treatment on whitefly were studied. Results: A total of 25 isolates of rhizobacteria formed a clear zone on solid chitin media. Two isolates, i.e., I.5 and I.21 isolates had the highest chitinolytic index. Based on sequence of 16S rRNA gene, the isolates of I.5 and I.21 were identified as Bacillus sp. and Bacillus cereus, respectively. The highest chitinolytic index and specific activity of I.5 isolate was 0.94 and 0.11 U mg −1 proteins, respectively. Maximum production of I.5 chitinase was occured after 36 h cultivation at 30°C and pH 7.0. The highest chitinolytic index and specific activity of I.21isolate was 0.75 and 0.114 U mg −1 proteins, respectively. Maximum production of I.21 chitinase was occured after 36 h cultivation at 55°C and pH 7.0. Cell culture and crude enzyme of the isolates were tested on chitin of B. tabaci and the effect was observed using a microscope and sterile water was used as a negative control. Hydrolytic observation showed that crude enzyme of I.21 isolate could degrade chitin of B. tabaci exoskeleton and the activity was better than that of I.5 isolate. Conclusion: Chitinase produced by Bacillus cereus I.21 strain has potential application as biocontrol agents for B. tabaci.

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Section: Discussionmentioning

confidence: 99%

Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Its Application as Biocontrol for Whitefly (Bemisia tabaci Genn.)

Mubarik¹,

Mahagiani²,

Anindyaputri³

et al. 2010

American Journal of Agricultural and Biological Sciences

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“…739, and in studies with B. amyloliquefaciens MET0908, with the aid of scanning electronic microscopy, the lytic activity of a β -1,3-glucanases on the Colletotrichum lagenarium hyphae was observed. Also, genetic engineering studies focused on the increase of antifungal activity of Bacillus strains have successfully achieved the insertion of codifying genes into lytic enzymes, such as gene ChiA of B. subtilis F29-3 in B. circulans, obtaining a more aggressive phenotype against Botrytis elliptica (Chen et al, 2004). In addition, Zhang et al (2012) significantly increased the antifungal activity of Burkholderia vietnamiensis P418 in the face of different fungal phytopathogens by the chromosomal insertion of the gene Chi113, from a B. Subtilis strain.…”

Section: Producción De Enzimas Líticasmentioning

confidence: 99%

“…739, y en estudios con B. amyloliquefaciens MET0908 se evidenció a través de microscopia electrónica de barrido la actividad lítica de una β -1,3-glucanasas sobre las hifas de Colletotrichum lagenarium. Además, estudios de ingeniería genética enfocados a incrementar la actividad antifúngica de cepas de Bacillus han logrado exitosamente la inserción de genes codificantes a enzimas líticas, tal como el gen ChiA de B. subtilis F29-3 en B. circulans, obteniendo un fenotipo más agresivo contra Botrytis elliptica (Chen et al, 2004). Además, Zhang et al (2012) incrementaron significativamente la actividad antifúngica de Burkholderia vietnamiensis P418 frente a diferentes fitopatógenos fúngicos mediante la inserción cromosómica de gen Chi113, proveniente de una cepa de B. subtilis.…”

Section: Production Of Siderophoresunclassified

El género Bacillus como agente de control biológico y sus implicaciones en la bioseguridad agrícola

Villarreal-Delgado

Villa-Rodríguez

Cira‐Chávez

et al. 2018

RMF

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Resumen. El género Bacillus se encuentra ampliamente distribuido en los agro-sistemas y una de sus principales aplicaciones es el control de enfermedades de cultivos agrícolas. En la presente revisión se describe y analiza al género Bacillus, y sus principales mecanismos de acción, tales como la excreción de antibióticos, toxinas, sideróforos, enzimas líticas e induciendo la resistencia sistémica, Abstract. The genus Bacillus is widely

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“…Further, mutants (W81M3 and W81M4) which have protease overproduction capacity exhibited noticeably improved biocontrol potential (Dunne et al 2000 ) . Similarly cloning of chitinase gene chiA of B. circulans WL-12 in strain B. subtilis F29-3 has shown that in comparison to the B. subtilis control strain, chiA expressing B. subtilis F29-3 exhibited a greater inhibition of spore germination of Botrytis elliptica (Chen et al 2004 ) . Hence it may be concluded that pathogen colonization and parasitism is a key mechanism adopted by many rhizobacteria to inhibit the fungal pathogen and such organisms have an edge over others in terms of effective control of the target pathogen (Fig.…”

Section: Parasitismmentioning

confidence: 99%

Biocontrol of Plant Pathogens Using Plant Growth Promoting Bacteria

Prashar

Kapoor

Sachdeva

2013

Sustainable Agriculture Reviews

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Food is the primary requirement for the survival of mankind. Therefore food has always been an issue since prehistoric times. Food production through agriculture is a key process to meet the world food demand. In the last four to fi ve decades global agricultural production has been drastically improved by introduction of improved crop varieties and development of modern agricultural tools. However, more than 25% of the crop yield is lost every year due to various kinds of plant diseases. Most critical plant diseases are caused by soilborne plant pathogens such as fungi, virus and nematodes. Effective measures are thus highly needed to avoid this crop loss. So far, the use of chemical pesticides has been the method of choice to tackle this problem. However, the unwarranted use of chemical tools to enhance the crop yield and control plant diseases has resulted in irreversible loss of soil quality along with serious health and environmental problems. Moreover, alternative controls are needed due to growing public concern about toxic agrochemicals and stringent laws. Microbes are a reasonable option to develop ecofriendly agricultural tools to replace chemical pesticides.Here we review the role of a special class of soil bacteria, called plant growth promoting rhizobacteria (PGPR). PGPR live in the rhizospheric sites in soil, i.e. in immediate vicinity of the plant roots, and exerts several bene fi cial effects on the plants, directly or indirectly. PGPR have inherent antagonistic properties against soilborne plant pathogens under natural conditions. The major points discussed here are: (1) biocontrol of plant pathogens as an alternative to chemical control methods.(2) The current market status of various biocontrol products with a noticeable increase of 80% market share in 5 years. (3) The success of PGPR to protect many plant species, against diverse range of plant pathogens. (4) Mechanisms underlying the control and inhibition of soilborne plant pathogens including antagonistic activities such as production of antibiotics, offering stiff competition to the pathogen for nutrients and niches in the rhizosphere, parasitism of the pathogen and induction of systemic resistance in the plants against diseases.

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Enhancement of the antifungal activity ofBacillus subtilisF29-3 by the chitinase encoded byBacillus circulans chiAgene

Cited by 31 publications

References 25 publications

Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Its Application as Biocontrol for Whitefly (<i>Bemisia tabaci</i> Genn.)

Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Its Application as Biocontrol for Whitefly (<i>Bemisia tabaci</i> Genn.)

El género Bacillus como agente de control biológico y sus implicaciones en la bioseguridad agrícola

Biocontrol of Plant Pathogens Using Plant Growth Promoting Bacteria

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