Enhancement of the growth of human endothelial cells by surface roughness at nanometer scale

Chung, Tsair-Wang; Liu, Der Zen; Wang, Sin Ya; Wang, Shoei‐Shen

doi:10.1016/s0142-9612(03)00361-2

Cited by 343 publications

(293 citation statements)

References 16 publications

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“…No significant difference was detected in cell number at day 3, however cell number increased to a significantly higher level within the laser treated samples at day 7. These results are in agreement with similar studies reporting the positive effect of laser modification of titanium alloys on the biological response [34,35]. Some groups report a significant difference in cell attachment with level of roughness [36], which was not found within the range of roughness encountered from the current study.…”

Section: Discussionsupporting

confidence: 93%

In vitrofibroblast and pre-osteoblastic cellular responses on laser surface modified Ti–6Al–4V

et al. 2014

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The success of any implant, dental or orthopaedic, is driven by the interaction of implant material with the surrounding tissue. In this context, the nature of the implant surface plays a direct role in determining the long term stability as physico-chemical properties of the surface affect cellular attachment, expression of proteins, and finally osseointegration. Thus to enhance the degree of integration of the implant into the host tissue, various surface modification techniques are employed. In this work, laser surface melting of titanium alloy Ti-6Al-4V was carried out using a CO 2 laser with an argon gas atmosphere. Investigations were carried out to study the influence of laser surface modification on the biocompatibility of Ti-6Al-4V alloy implant material. Surface roughness, microhardness, and phase development were recorded. Initial knowledge of these effects on biocompatibility was gained from examination of the response of fibroblast cell lines, which was followed by examination of the response of osteoblast cell lines which is relevant to the applications of this material in bone repair. Biocompatibility with these cell lines was analysed via Resazurin cell viability assay, DNA cell attachment assay, and alamarBlue metabolic activity assay. Laser treated surfaces were found to preferentially promote cell attachment, higher levels of proliferation, and enhanced bioactivity when compared to untreated control samples. These results demonstrate the tremendous potential of this laser surface melting treatment to significantly improve the biocompatibility of titanium implants in vivo.

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Section: Discussionsupporting

confidence: 93%

In vitrofibroblast and pre-osteoblastic cellular responses on laser surface modified Ti–6Al–4V

et al. 2014

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“…The adhesion of living cells was enhanced by the nanostructured TNT layer, as evidenced by the rapid spreading curves recorded by OWLS and the increased resistance to hydromechanical removal (by washing). The enhancement of cell adhesion is in line with the general observations of cell adhesion being enhanced by rough substrata [65][66][67]. We can exclude any effects of electrostatic interactions between protein and substratum, because at the ionic strength of the medium we used the electrostatic interactions are negligible in comparison with the Lewis acid/base interactions [64].…”

Section: Chemical States and The Origin Of Adsorption And Adhesion Ensupporting

confidence: 83%

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Nádor

Orgován

Fried

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Please cite this article in press as: J. Nador, et al., Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips, Colloids Surf. a b s t r a c tA new type of titanate nanotube (TNT) coating is investigated for exploitation in biosensor applications. The TNT layers were prepared from stable but additive-free sols without applying any binding compounds. The simple, fast spin-coating process was carried out at room temperature, and resulted in well-formed films around 10 nm thick. The films are highly transparent as expected from their nanostructure and may, therefore, be useful as coatings for surface-sensitive optical biosensors to enhance the specific surface area. In addition, these novel coatings could be applied to medical implant surfaces to control cellular adhesion. Their morphology and structure was characterized by spectroscopic ellipsometry (SE) and atomic force microscopy (AFM), and their chemical state by X-ray photoelectron spectroscopy (XPS). For quantitative surface adhesion studies, the films were prepared on optical waveguides. The coated waveguides were shown to still guide light; thus, their sensing capability remains. Protein adsorption and cell adhesion studies on the titanate nanotube films and on smooth control surfaces revealed that the nanostructured titanate enhanced the adsorption of albumin; furthermore, the coatings considerably enhanced the adhesion of living mammalian cells (human embryonic kidney and preosteoblast).

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“…Proofs of these statements are presented in publications [9][10][11][12][13][14][15][16][17][18]. There are materials similar to the structure of the tissue.…”

Section: An Important Aspect In the Development Of An Effective Treatmentioning

confidence: 98%

Bio-Inspiered Blood-Contacting Materials Elaborated For The Heart Assist System

Major¹

2015

Archives of Metallurgy and Materials

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I dedicate this work to my Father, who is also my Mentor.He has enabled me to execute my professional passion.The paper presents the main achievements of the author on the development of blood contacting materials. The main objective of the work is to elaborate materials dedicated for the heart support systems. Appropriately designed biomaterial surfaces enable fully controlled cellular differentiation, proliferation, and even restoration of the tissue structure on solids. The paper presents two approaches to modify the surface, which can control the life processes of tissue. The first solution considers the topography in the form of cell niches. The main objective of the study is a modified surface of thin films deposited on the polymer substrate constituting the microenvironment for the cells caused by residual stress and optimized stiffness of the surface using the plasma methods. The research hypothesis was the plasma surface modification method generating a controlled contribution of residual stress in the coating affect the surface topography in the form of nano-wrinkles similar to the niches in the tissue environment. Topography and stiffness of the surface coating allows the targeted cellular differentiation. The properly formed surface topography effectively inhibits blood clotting processes. The second solution considers implementation of self-organizing feature of extracellular matrix like coatings and selective cell mobilization. The multiscale analysis and phenomenologic description were performed to experimental research. For this purpose, the deposition method was based on electrostatic interactions in polyelectrolytes. This type of cell-polymer structure imitate the native structures.Keywords: Surface modification, cell-material interaction, tissue precursors, materials for regenerative medicine Praca przedstawia najważniejsze osiągniecia autora dotyczące rozwoju materiałów do kontaktu z krwią. Głównym celem prowadzonych prac są materiały o przeznaczeniu w komorach wspomagania serca. Odpowiednio zaprojektowana powierzchnia biomateriału umożliwia w pełni kontrolowane różnicowanie komórkowe, proliferację i nawet odtworzenie struktury tkanki na ciele stałym. W pracy przedstawiono dwa podejścia modyfikacji powierzchniowej, które pozwalają sterować procesami życio-wymi tkanki. Pierwszym rozwiązaniem są powłoki o topografii nisz komórkowych. Celem głównym badań jest zmodyfikowana powierzchnia materiałów cienkowarstwowych nałożonych na podłoże polimerowe stanowiąca mikrośrodowisko dla wychwytu i kontrolowanego różnicowania komórek uzyskane przez odpowiedni udział naprężeń własnych i zoptymalizowaną sztywność powierzchniową. Ukierunkowana mikrostrukturą i właściwościami powierzchniowymi monowarstwa hamuje procesy wykrzepiania krwi. Drugie rozwiązanie dotyczy powłok o strukturze macierzy zewnątrzkomórkowej. Celem badań jest wieloskalowa analiza i opis fenomenologiczny samoorganizujących się powłok z funkcją selektywnej mobilizacji komórkowej. Jest to nowoczesne podejście na poziomie badań eksperymentalnyc...

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Enhancement of the growth of human endothelial cells by surface roughness at nanometer scale

Cited by 343 publications

References 16 publications

In vitrofibroblast and pre-osteoblastic cellular responses on laser surface modified Ti–6Al–4V

In vitrofibroblast and pre-osteoblastic cellular responses on laser surface modified Ti–6Al–4V

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Bio-Inspiered Blood-Contacting Materials Elaborated For The Heart Assist System

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