“…However, its application is often limited by high retention time because of low methane production and biodegradability (Bolzonella et al, 2005) due to the hydrolysis step of sewage sludge treatment (Kuglarz et al, 2013). Different pre-treatments, such as ultrasonic, ozone, mechanical, alkaline, and thermal processes (Braguglia et al, 2012;Chi et al, 2011;Lehne et al, 2001;Martin et al, 2015;Ruffino et al, 2015), have been proposed to enhance the hydrolysis and biomethanisation of sewage sludge.…”