Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method

Akkol, Esra Küpeli; Açıkara, Özlem Bahadır; Süntar, İpek; Çitoğlu, Gülçin Saltan; Keleş, Hikmet; Ergene, Burçin

doi:10.1016/j.jep.2011.07.029

Cited by 46 publications

(60 citation statements)

References 28 publications

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“…1-8). The HPLC method described by Küpeli Akkol et al was improved and used [22]. The results of our analyses confirmed the presence of compounds observed previously (hyperoside (2) and chlorogenic acid (8) [22][23][24]), and further chromatographic separation and later HPLC analysis also confirmed the presence of other phenols (1, 3-7).…”

Section: Resultssupporting

confidence: 87%

“…Mouse ear edema induced with topically-applied TPA is also an excellent acute inflammation animal model, closely Scorzonera species were in focus of our previous research touching testing of analgesic, anti-inflammatory, and wound healing activities. Aerial part extracts of Scorzonera showed significant inhibitory effects in carrageenan and PGE 2 induced hind paw edema model in vivo [21][22][23]. The concentrations of extracts showing the activity was 100 mg/kg of body weight of mice, and the effect was comparable with indomethacine used as the standard at the some concentration.…”

Section: Resultsmentioning

confidence: 89%

“…In order to verify traditional usage of Scorzonera species, anti-nocieptive, anti-inflammatory, and wound healing activities have been evaluated and promising results have been obtained in our previous research [21][22][23][24]. The aim of the current study was to evaluate the ability of Scorzonera extracts to inhibit TNF-α and IL-1β production and NF-κB nuclear translocation in LPS-stimulated THP-1 macrophages, which may be responsible for the observed anti-inflammatory activity.…”

Section: Introductionmentioning

confidence: 94%

“…and Mey.) DC., S. parviflora Jocq., and S. tomentosa L., which displayed potent anti-inflammatory activity in vivo test models in our previous researches were selected for activity tests [22][23][24]. HPLC analysis of the tested Scorzonera extracts was also performed with aim to elucidate their composition.…”

Section: Introductionmentioning

confidence: 99%

“…HPLC analysis of the tested Scorzonera extracts was also performed with aim to elucidate their composition. HPLC analytical method previously published by [22] was optimized and composition of the extracts was tested qualitatively to select main compounds for activity testing. According to this and previous work, eight phenolics: quercetin-3-O-β-D-glucoside (1), hyperoside (2), hydrangenol-8-O-glucoside (3), swertisin (4), 7-methylisoorientin (5), 4,5-O-dicaffeoyl-quinic acid (6), 3,5-O-dicaffeoyl-quinic acid (7), and chlorogenic acid (8), which were isolated as the major phenolic compounds in current study, and terpenoids 9-16, which have been isolated previously [taraxasterol acetate (9), lupeol (10), lupeol acetate (11), β-sitosterol (12), 3-β-hydroxy-fern-8-en-7-one-acetate (13), urs-12-en-11-one-3-acetyl (14), 3-β-hydroxy-fern-7-en-6-one-acetate (15), and olean-12-en-11-one-3-acetyl (16)] were also tested for their inhibitory effects on the TNF-α and IL-1β production.…”

Section: Introductionmentioning

confidence: 99%

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Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-κB Activation, Showing Anti-Inflammatory Effect in Vitro

et al. 2015

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Scorzonera species are used in different folk medicines to combat many diseases, including the illnesses connected with inflammation. Previous experiments showed anti-inflammatory activity of Scorzonera extracts in vivo. S. latifolia, S. cana var. jacquiniana, S. tomentosa, S. mollis ssp. szowitsii, S. eriophora, S. incisa, S. cinerea, and S. parviflora extracts were, therefore, evaluated for their inhibitory activities of TNF-α and IL-1β production, and NF-κB nuclear translocation in THP-1 macrophages. The HPLC analysis was carried out to elucidate and to compare the composition of these extracts. Major compounds of the tested extracts have been isolated using different chromatographic techniques and further tested for their inhibitory activities on TNF-α and IL-1β production. Several extracts showed promising anti-inflammatory activity in these in vitro tests. Results of HPLC analysis revealed chlorogenic acid as a compound present in all tested extracts. Hyperoside, quercetin-3-O-β-D-glucoside and rutin were also present in varying amount in some Scorzonera species analyzed. Furthermore, eight phenolics which were identified as quercetin-3-O-β-D-glucoside (1), hyperoside (2), hydrangenol-8-O-glucoside (3), swertisin (4), 7-methylisoorientin (5), 4,5-O-dicaffeoyl-quinic acid (6), 3,5-di-O-caffeoyl-quinic acid (7), and chlorogenic acid (8) have been isolated as major phenolic compounds of the tested extracts and, together with eight terpenoids (9-16) previously obtained from different Scorzonera species, have been tested for the inhibition of TNF-α production, unfortunately with no activity comparable with standard.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-κB Activation, Showing Anti-Inflammatory Effect in Vitro

et al. 2015

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Development of an LC–MS/MS method for quantification of two pairs of isomeric flavonoid glycosides and other ones in rat plasma: Application to pharmacokinetic studies

Zhang

Xie

Wang

et al. 2017

Biomedical Chromatography

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A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of six flavonoid glycosides - isoorientin (1), orientin (2), 2″-O-β-d-xylopyranosyl isoorientin (3), 2″-O-β-d-xylopyranosyl isovitexin (4), 6-C-l-α-arabipyranosyl vitexin (5) and vitexin (6) - in rat plasma using isoquercitrin as the internal standard (IS). Plasma samples were prepared by a one-step protein precipitation with acetonitrile. Chromatographic analysis was carried out on a 25 cm C column with a gradient mobile phase consisting of acetonitrile and 0.1% aqueous formic acid. Six analytes and IS were detected through electrospray ionization in negative-ion selection reaction monitoring mode. The mass transitions were as follows: m/z 447.2 → 327.0 for 1, m/z 447.2 → 327.0 for 2, m/z 579.3 → 458.9 for 3, m/z 563.0 → 293.1 for 4, m/z 563.0 → 353.0 for 5, m/z 431.1 → 311.1 for 6, and m/z 463.1 → 300.2 for IS. Calibration curves exhibited good linearity (r > 0.9908) over a wide concentration range for all compounds. Intra- and inter-day precision (RSD, %) at four different levels were both <14.2% and the accuracy (RE, %) ranged from -11.9 to 12.0%. The extraction recoveries of the six components ranged from 88.2 to 103.6%. The validated assay was successfully applied to the pharmacokinetic studies of the six components in male rat plasma after intravenous administration of total flavonoids of Scorzonera austriaca Wild.

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Effect of Argemone mexicana on Local Edema and LPS‐Induced Neuroinflammation

Monterrosas-Brisson

Zagal-Guzmán

Zamilpa³

et al. 2021

Chemistry & Biodiversity

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Argemone mexicana L. is a widely used plant in Mexican traditional medicine to treat inflammatory and nervous medical conditions. It has been subjected to several pharmacological and chemical studies in which acute anti‐inflammatory activity is indicated. This work aimed at finding an extract and fraction with anti‐inflammatory activity by means of 2‐O‐tetradecanoylphorbol‐13‐acetate (TPA)‐induced auricular edema. Afterward, the extract and the fraction were tested on neuroinflammation caused by lipopolysaccharides (LPS). Treatments obtained from A. mexicana included the methanolic extract (AmMeOH), a fraction extracted with ethyl acetate (AmAcOEt), and four sub‐fractions (AmF‐1 to AmF‐4), which were evaluated in auricular edema with the TPA assay. Both treatments with the most significant inhibitory effect were employed to test these in the LPS neuroinflammation model. AmAcOEt and AmF‐3 induced a higher inhibition of edema (%), and both diminished ear inflammation when viewed under a microscope. These treatments also raised an increase in spleen, but not in brain of mice with neuroinflammation. They were able to decrease the concentration of proinflammatory cytokines (TNF‐α, IL‐1β, and IL‐6) in both organs. Furthermore, the accumulation of amyloid‐β (Aβ) in hippocampus was not visible. AmF‐3 contains the flavonoids isoquercetin, luteolin, and rutin, the former being the most concentrated.

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Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method

Cited by 46 publications

References 28 publications

Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-κB Activation, Showing Anti-Inflammatory Effect in Vitro

Turkish Scorzonera Species Extracts Attenuate Cytokine Secretion via Inhibition of NF-κB Activation, Showing Anti-Inflammatory Effect in Vitro

Development of an LC–MS/MS method for quantification of two pairs of isomeric flavonoid glycosides and other ones in rat plasma: Application to pharmacokinetic studies

Effect of Argemone mexicana on Local Edema and LPS‐Induced Neuroinflammation

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